The invention belongs to the technical field of food preservation, particularly relates to a fresh freezing solution special for food sterilization and preservation, and a preparation method thereof,and solves the problems that a traditional fresh freezing solution in the prior art causes cell membrane damage, poor taste, reduced nutritional ingredients and the like. The fresh freezing solution special for food sterilization and preservation comprises the following raw materials in parts by weight: 2-5 parts of a plant extract, 2-5 parts of an insect extract, 5-9 parts of calcium chloride, 1-2 parts of lactic acid, 8-15 parts of ethanol and 50-70 parts of water, wherein the plant extract is obtained by extracting dandelion and radix sophorae flavescentis; and the insect extract is obtained by extracting ground beetles and aspongopus. The fresh freezing solution is prepared by compounding the plant extract, the insect extract, the calcium chloride, the lactic acid, the ethanol and thewater in the invention; and the obtained fresh freezing solution is simple in preparation process, low in preparation cost, stable in performance, low in working temperature, low in volatility and lowin corrosivity, can improve the product quality and prolong the storage period when being used for food preservation, and is pollution-free.