The invention discloses traditional Chinese medicine distiller's yeast. The distiller's yeast comprises red-knees herb, gastrodia elata leaves, xanthium sibiricum leaves, mulberry leaves, hyacinth bean leaves, lophatherum gracile roots and shoots, mint, radix angelicae pubescentis, radix paeoniae alba, sichuan lovage rhizome, asarum, scutellaria baicalensis peel, radix aucklandiae, herba ephedra,cinnamon, dried tangerine peel, cyperus rotundus, schisandra chinensis, cortex cinnamomi, galanga, fresh ginger, cassia occidentalis, purple perilla, saruma henryi, almonds, clove, rhizoma atractylodis macrocephalae, radix ophiopogonis, licorice root, monkshood, radix puerariae, fructus aurantii and long-shaped rice. In addition, the invention discloses a preparing method of the traditional Chinese medicine distiller's yeast. The liquor brewed through the traditional Chinese medicine distiller's yeast prepared through the process is strong and complicated in flavor, thick and rich in taste, slightly bitter in taste, sweet in aftertaste after the bitter taste and free of mouth burning or drunk feeling, and people who wake up after getting drunk still feel spirited and have faint scent in mouth.