The invention provides a xenon target preparation device for reactor irradiation production of I-125 (iodine-125). The xenon target preparation device for the reactor irradiation production of the I-125 comprises a gas backflow tank, a target opening device, a raw material tank, a storage device, a filling weld device and a vacuum pump, which are arranged on a rack and fixedly connected with the rack, wherein a pressure gage is fixed on the gas backflow tank, the gas backflow tank is connected with the target opening device, the raw material tank, the storage device, the filling weld device, the vacuum pump and the pressure gage, a liquid nitrogen pump is arranged below the rack, the liquid nitrogen pump is connected with the target opening device, the filling weld device and the storage device, the filling weld device is connected with a fusion welding power source, and the target opening device, the filling weld device, the vacuum pump, the liquid nitrogen pump and the fusion welding power source are respectively connected with a controller in electrical mode. The xenon target preparation device for the reactor irradiation production of the I-125 has target opening and welding functions, not only can use raw material xenon gas to prepare different packing volumes and specifications of xenon targets, but also can be integrated with a production system of the I-125, and repeatedly manufactures recycled 124Xe (xenon) into the targets on line so as to reuse the 124Xe. The xenon target preparation device for the reactor irradiation production of the I-125 is compact and small, and convenient to operate.