30 results about "Heparan Sulfate Proteoglycan" patented technology

Provided herein are improved rAAV (e.g., rAAV2, rAAVrh8R, etc.) for enhanced gene therapy of ocular disorders or CNS disorders wherein the rAAV comprise one or more substitutions of amino acids that interact with heparan sulfate proteoglycan. The invention provides methods for improved transduction of retinal cells and methods for treating ocular diseases with improved compositions of rAAV particles. Further provided herein are improved recombinant adeno-associated virus (rAAV) (e.g., rAAV2, rAAVrh8R, etc.) for enhanced gene therapy of disorders of the CNS. The invention provides methods for delivering the rAAV to the CNS, methods for treating disorders of the CNS with improved compositions of rAAV particles, and kits for delivering the rAAV to the CNS and / or treating a CNS disorder.

Provided herein are improved rAAV (e.g., rAAV2, rAAVrh8R, etc.) for enhanced gene therapy of ocular disorders or CNS disorders wherein the rAAV comprise one or more substitutions of amino acids that interact with heparan sulfate proteoglycan. The invention provides methods for improved transduction of retinal cells and methods for treating ocular diseases with improved compositions of rAAV particles. Further provided herein are improved recombinant adeno-associated virus (rAAV) (e.g., rAAV2, rAAVrh8R, etc.) for enhanced gene therapy of disorders of the CNS. The invention provides methods for delivering the rAAV to the CNS, methods for treating disorders of the CNS with improved compositions of rAAV particles, and kits for delivering the rAAV to the CNS and / or treating a CNS disorder.

Polypeptides of the neuregulin (NRG) heparin binding domain (N-HBD) and nucleic acids coding therefor are disclosed. In particular, fusion polypeptides are produced that comprise, as a targeting structure, a N-HBD polypeptide, fragment, homologue or functional derivative and a protein to be targeted. This is fused to a polypeptide or peptide being targeted (Ptrg) to cell surfaces rich in heparan sulfate proteoglycans to either activate or inhibit interactions at tyrosine kinase receptors. Such products are used to treat diseases or conditions where either agonism or antagonism at tyrosine kinase receptors has beneficial effects, including cancer and a multitude of diseases of the nervous system.

A method for enhancing chondrogenesis in a subject in need thereof comprises administering to the subject an effective amount of a pharmaceutical composition comprising a growth factor bound to hyaluronic acid (HA)-based hydrogel particles (HGPs). The hyaluronic acid (HA)-based hydrogel particles (HGPs) are conjugated with a heparan sulfate proteoglycan (HSPG). Related medicaments and methods for preparing the medicaments are provided.

The invention discloses a cross-linked hydrogel used for culturing muscle stem cells, a preparation method and application thereof, and belongs to the technical field of biological food materials. In the present invention, collagen is dissolved to form a solution, and then a certain amount of alginate and heparan sulfate proteoglycan is added to mix it with the collagen solution; then ε-PL and TG enzyme are added to the solution, stirred evenly, and The slurry is placed in a mold to cross-link, resulting in a hydrogel. The hydrogel of the present invention connects collagen, polylysine and heparan sulfate proteoglycans through TG enzyme to form covalent crosslinks, and through the physical electrostatic interaction between polylysine and alginate to form Dense three-dimensional "egg carton" network structure. The use of this hydrogel can enhance the absorption of nutrients by muscle stem cells and facilitate their growth. Double-crosslinked network hydrogels have potential as scaffolds for muscle stem cell growth in stem cell culture meat.

Provided is a method for inducing differentiation of pluripotent stem cells into somatic cells using a medium containing heparin binding growth factor, characterized by comprising contacting startingcells with a conjugate in which a laminin E8 fragment is bound to a fragment containing the growth factor-binding site of heparan sulfate proteoglycan. According to the present invention, differentiation of pluripotent stem cells into arbitrary somatic cells can be induced at a high efficiency.

The invention discloses a tissue engineering osteochondral composite support and a preparation method thereof, belonging to the technical field of biomaterials. The support is a multi-layer integrated structure, which is composed of a cartilage tissue support layer, a cartilage tissue calcification layer, a porous cell separation membrane and a bone tissue support layer. The cartilage tissue scaffold layer is inoculated with chondrocytes and introduced growth factors that promote chondrocyte formation to promote the growth of chondrocytes; the main raw materials such as heparan sulfate proteoglycan with good biocompatibility and degradability are selected, and cross-linking reaction, freeze-drying, and directional Pore-forming, multi-layer composite, complete integration and other methods to obtain functional multi-layer integrated tissue engineering osteochondral composite scaffold with good mechanical properties. The invention adopts micro-nano hydroxyapatite crystals and degradable non-stoichiometric polyethylene glycol / polyε-caprolactone nano-coaxial short fibers and heparan sulfate proteoglycan / oxidized sodium alginate grafted with RGD Compounded with N-succinyl chitosan, it is mainly used for repairing full-thickness defects of articular cartilage and subchondral bone.