The invention discloses hippophae rhamnoides fruit juice and a preparation method thereof. The hippophae rhamnoides fruit juice comprises the following formula materials: hippophae rhamnoides fruits, fresh Chinese wolfberry fruits, starch, o-benzoyl sulfimide, malic acid, high fructose corn syrup, additives, a stabilizer, edible salt, honey, white granulated sugar and purified water, and the additives comprise citric acid, sodium citrate and xanthan gum. According to the hippophae rhamnoides fruit juice and the preparation method thereof, various formulas such as the fresh Chinese wolfberry fruits, the starch, the o-benzoyl sulfimide, the malic acid, the high fructose corn syrup, the additive, the stabilizer, the edible salt, the honey and the white granulated sugar are added into the hippophae rhamnoides fruit juice, the taste of the sea-buckthorn fruit juice is diversified, meanwhile, the sour and astringent taste carried by the sea-buckthorn fruits is effectively removed, so that the taste of the sea-buckthorn fruits is improved, the drinking requirements of drinkers are met, the fresh Chinese wolfberry fruits, the malic acid and the honey are added into the sea-buckthorn fruit juice, and the nutritional value of the sea-buckthorn fruit juice is further increased, and thus the beverage is beneficial to the human body.