The invention provides a weight-losing composition containing mesenchymal stem cells as well as a preparation method and application of the weight-losing composition, and belongs to the technical field of medicines. Eurotium cristatum and
lactobacillus plantarum are used for fermenting white tea and dark
green tea, probiotics are collected and embedded to prepare embedded probiotics, tea residues are subjected to
supercritical fluid extraction, oxidation and extraction to prepare a tea brown composition, fetal calf serum,
dexamethasone,
penicillin,
streptomycin, magnetic particles and
interleukin-2 are added into a DMEM culture medium, a culture medium is prepared, and the white tea and dark
green tea are prepared. The preparation method comprises the following steps: carrying out low-frequency
magnetic field culture and separation on adipose-
derived stem cells to obtain a
stem cell solution, embedding to obtain
stem cell microspheres, freeze-
drying supernate to obtain a paracrine culture substance, and uniformly mixing the paracrine culture substance with a
fermentation product, a brown composition and embedded probiotics to obtain the weight-losing composition containing the adipose-
derived stem cells. The health-care food not only has good anti-oxidation, anti-inflammatory, antibacterial and
blood sugar and blood fat regulating effects, but also has a good weight-losing effect, and has a wide application prospect.