The invention provides natural bamboo fiber shirt fabric which has silky gloss, good feel and drapability, and is rich in graceful feeling, antibiosis, moisture-adsorption and ventilating. Warps and wefts of the fabric are both natural fiber / bamboo viscose blended yarns, the blending ratio of the warps and the wefts is 1:1, the warp density is 512 / 10cm, and the weft density is 338.5 / 10cm. A manufacture method of the natural bamboo fiber shirt fabric comprises the following steps: a, blending: opening picking, carding cotton, drawing out, roving and spun yarn; b, drum yarn dying: tube loosening, pretreatment, dying, dehydration and drying, wherein the pretreatment comprises bleaching and enzyme treatment; during bleaching: soda ash is 1-3g / L, hydrogen peroxide is 1-2g / L, the temperature is 95 DEG C, the time is 35-45min; and during the enzyme treatment, HontonezymePA is 1-1.5g / L, pH value is 4.5-5.5, the bath ratio is 1:15, and the time is 25-35min; and during dying, the drum yarn density is 0.33-0.35g / cm<3>, and MegafixB type reactive dying is adopted; c, weaving: winding, warp pulp connection, drawing in or warp tying and weaving; and d, after treatment: singeing, desizing, sizing and preshrinking.