The invention discloses a sterile tissue and a production process thereof, and belongs to the technical field of tissue production. The tissue is prepared from, by weight, 20-60% of bleached sulfate softwood pulp, 30-70% of CPMP and 0-10% of broken paper pulp. According to the method, by adopting an appropriate principle and ratio, wet strength agents with low organic chlorine, biodegradable non-toxic fungicides and FDA-authenticated coatings, adopting forming equipment with a low vertical-horizontal tension ratio, a drying and sterilization process at the temperature higher than 40 DEG C andsafe non-toxic chemicals, and combining appropriate pressing printing processes, the produced tissue has the advantages of being low in quantification, low in vertical-horizontal tension ratio, sterile, environmentally friendly and high in water absorption performance.