The invention provides preparation type milk-containing beverage with an anti-fatigue function, and a preparation method thereof. The milk-containing beverage comprises the following raw material components in parts by weight: 320 to 400 parts of raw goat milk, 30 to 36 parts of white granulated sugar, 1 to 5 parts of apple juice, 1 to 2 parts of orange juice, 2 to 5 parts of a seaweed extract, 1 to 2 parts of celery juice, 1 to 2 parts of citric acid, 1 to 2 parts of lactic acid, 0.3 part of sodium citrate, 0.08 part of acesulfame potassium, 0.5 part of a stabilizer and 0.3 part of potassium sorbate. The preparation method of the milk-containing beverage comprises the steps of preparing the apple juice, preparing the orange juice, preparing the celery juice, preparing the seaweed extract, weighing the materials, purifying milk, mixing, homogenizing, sterilizing at high temperature and filling. The milk-containing beverage provided by the invention improves the odor of the milk-containing beverage and has higher stability.