The invention discloses a preparation method of a bimodal tissue Mg-6Zn-0.45Zr alloy. The method comprises alloy heat treatment and alloy hot working, wherein the preservation temperature of the alloyheat treatment is 330 + / -5 DEG C, the heat preservation time of the alloy is 10 + / -0.5 hours, and the cooling mode is air cooling; and the temperature of the alloy hot working is 250 + / -5 DEG C, theheat preservation time before the alloy hot working is 10 + / -1 minutes, the alloy hot working mode adopts small-pass strain high-strain-rate three-direction forging, the forging equipment is an air hammer, secondary heating is not repeated among the passes, the pass strain capacity is 10 + / -0.5%, the deformation pass is 24 passes, and the cooling mode is the air cooling. According to the preparation method, the bimodal tissue Mg-6Zn-0.45Zr alloy with controllable crystalline grain distribution can be prepared, and the bimodal tissue Mg-6Zn-0.45Zr alloy has excellent comprehensive mechanical properties; the temperature drop caused by heat dissipation is made up by utilizing the temperature rise effect of high-speed deformation, no heating is needed among the passes, and the method is shortin machining period and is suitable for producing large-size block body of the Mg-6Zn-0.45Zr alloy.