The invention relates to a bionic collagen matrix with a
density gradient double-layer structure, and belongs to the technical field of medical materials. The collagen matrix takes telopeptide collagen derived from mammalian
dermis or
achilles tendon as a main component, and comprises a double-layer structure of a compact layer and a loose layer, and the compact layer is smooth in surface and compact in structure, so that
cell migration can be blocked, and a certain protection effect is achieved; the loose layer is of a porous structure,
cell ingrowth and blood clot stabilization are facilitated, and a support is provided for
tissue repair. The preparation method of the
biological membrane comprises the following steps: preparing telopeptide-removed
collagen gel, preparing
collagen sponge, preparing a collagen tissue regeneration membrane, and compounding the collagen tissue regeneration membrane and the
collagen sponge into an integrated double-layer structure through bonding,
drying and crosslinking. The double-layer structure simultaneously meets the double requirements of barrier protection and tissue regeneration, the process is simple and controllable, and the double-layer structure is suitable for clinical scenes such as oral
soft tissue repair,
alveolar ridge reconstruction and tooth extraction pit coverage.