The invention discloses high-efficiency enhancing night-benefiting toothpaste with functional active ingredients added and a preparation method. The high-efficiency enhancing night-benefiting toothpaste is prepared from light calcium carbonate, decolorized animal bone meal, xylitol, glycerinum, polyethylene glycol-400, negative ionized hydrated silica nanometer powder, 2-acyl-oxygen-based bond sodium sulfonate, greening spermaceti wax, grapefruit compound green tea extracts, bacterium removing agent, cellulose gum, carboxymethyl cellulose sodium, vitamin C, tetrasodium pyrophosphate, screwtree root extracts, honeysuckle extracts, holly leaf extracts, liriope spicata extracts, chitosan-modified organic acid salt with molecular weight of 0.3-0.5 million D and other raw materials. Chitosan friction agent with molecular weight of 0.3-0.5 million D and adsorption carriers of plant nanometer small molecules are added to the toothpaste, the damage of the toothpaste to teeth and oral cavity soft tissue is avoided, plant nanometer small molecules are dispersed, and the toothpaste has wider and more durable bacterial restraining and killing effects, can effectively conduct oral cavity nursing and has an effect on highly efficiently enhancing oral cavity nursing at night.