The invention relates to a twice-mallard-reaction-type chicken-flavor material, as well as a formulation and a preparation method thereof, and belongs to the field of foods. The formula of the twice-mallard-reaction-type chicken-flavor material comprises L-cysteine, vitamin B1, DL-methionine, glucose, chicken oil, phosphoric acid, chicken meal, yolk meal, onions, gingers, peppers, a yeast extract,edible salt, monosodium glutamate, sugar, disodium 5'-ribonucleotide, denatured starch, and xanthan gum. The formula is rich in ingredients; and the ingredients are easy to obtain, and relatively lowin costs. And the ingredients have relatively good effects under the proportion of the formula. The preparation method of the twice-mallard-reaction-type chicken-flavor material comprises the following steps of mixing main raw materials with a reaction base for the first mallard reaction according to the formula; carrying out first mallard reaction so as to obtain a primary flavoring material; mixing auxiliary materials with the primary flavoring material, and carrying out homogenization so as to obtain the twice-mallard-reaction-type chicken-flavor material. The preparation method is simple,rapid, safe, and healthy; moreover, the processes can be easily controlled so that inter-batch differences of products can be effectively reduced. The twice-mallard-reaction-type chicken-flavor material prepared by the preparation method is small in heat-sensitive substance loss, high in flavor substance content, and strong in sense of hierarchy and fidelity.