The present invention discloses a microgel based on acryloyl
gelatin, a preparation method and an application thereof. A microfluidic method is used in combination with an online photopolymerization reaction to prepare an acryloyl
gelatin microgel in high
throughput, and the application thereof in the encapsulation of bioactive substances. First, acryloyl
gelatin microdroplets are prepared in high
throughput using
droplet microfluidics technology, and then a visible
light source matched with a
photoinitiator is placed above the
microfluidics pipeline to achieve online
continuous production of GelA microdroplets and solidify to form microgel particles. For online encapsulation of substances, it is only necessary to pre-mix the active substances with the aqueous phase. The present invention combines the advantages of
droplet microfluidics technology for high
throughput production of droplets of
uniform size, and also has the advantages of GelA's low
viscosity, high photopolymerization efficiency and good
biocompatibility. It can be used for high-efficiency and high-quality production of biocompatible microgels. The preparation process is also biocompatible and can meet the needs of online encapsulation of biomolecules and cells.