The invention relates to the technical field of
biochemical detection, and particularly discloses an
agkistrodon blomhoffii ussurensis defibrase HPLC detection method and application thereof. According to the HPLC detection method, a mobile phase A, a mobile phase B and a mobile phase C are adopted for
elution; the mobile phase A is a 20-25 mM
aqueous solution of
sodium dihydrogen
phosphate, and the pH value of the
aqueous solution of
sodium dihydrogen
phosphate is 5.8-7.0; the mobile phase B is
acetonitrile; and the mobile phase C is an
aqueous solution of
trifluoroacetic acid. Octadecylsilane chemically bonded silica is used as a filling agent in a reversed-phase column, and
gradient elution is carried out. By establishing a purity determination method which is simple, convenient and sensitive and has good specificity, accuracy and durability, the quality of the
agkistrodon blomhoffii ussurensis defibrase can be effectively controlled, and the quality of the
agkistrodon blomhoffii ussurensis defibrase is stable, controllable, efficient and safe. Meanwhile, the method can also be used for identifying defibrases from different
snake venom sources, and lays a foundation for improving quality standards of defibrase raw materials and preparations thereof.