The invention discloses a bismuth compound with 2-acetyl-3-ethylpyrazine thiosemicarbazone as a ligand and a synthesis method of the bismuth compound. The method comprises the steps of dissolving 2-acetyl-3-ethylpyrazine in methyl alcohol to prepare a solution; taking thiosemicarbazone into methyl alcohol, then dropping the prepared solution into a methyl alcohol solution containing the thiosemicarbazone, conducting a backflow mixing reaction, cooling to the room temperature, afterwards, pouring the solution into a beaker for volatilization, filtering an obtained precipitate, afterwards, utilizing absolute ethyl alcohol for washing of 2-3 times, and obtaining the ligand after drying; taking BiCl3 to be dissolved into the methyl alcohol solution, then dropwise adding the methyl alcohol solution into an ethanol solution containing the ligand, conducting a backflow mixing reaction, filtering the solution after the reaction into the beaker, replenishing methyl alcohol, utilizing preservative film for sealing, utilizing a needle for punching out several holes, and conducting volatilization for several days at 4 DEG C to obtain a bismuth compound crystal. The synthesized bismuth compoundis subjected to in-vitro proliferation inhibition activity experiments, it is proved that for cancer cells, the activity of the pure 2-acetyl-3-ethylpyrazine thiosemicarbazone ligand is not high, andafter the ligand is coordinated with bismuth ions, the activity is greatly improved.