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The invention relates to the processing field of the production process of biological organic fertilizers, and particularly relates to a silkworm manure biological organic fertilizer and a preparation method thereof. According to the technical scheme, the silkworm manure biological organic fertilizer comprises the following ingredients in parts by weight: 60 to 80 parts of fermented silkworm manure, 10 to 20 parts of fermented tree leaves, 30 to 50 parts of fermented rice bran, 1 to 3 parts of fermented animal excrement and 15 to 20 parts of rice washing water. The silkworm manure biological organic fertilizer has characteristics that simplicity in production is realized, the cost is low, the fertilizer efficiency is high, by adding the fermented silkworm manure, the fermented tree leaves and the fermented rice bran, the soil can be regulated, the vitality of microorganisms in the oil can be activated, the hardening of the soil can be overcome, and the air permeability of the soil can be improved. The loss and evaporation of water can be reduced, the drought pressure can be alleviated, the fertilizer can be maintained, the use of chemical fertilizer can be reduced, the harm of salt and alkali can be alleviated, under the situation that the use amount of the chemical fertilizer is reduced or the chemical fertilizer is gradually substituted, the animal and livestock excrement and plant fermented fertilizer after being fermented by the microorganisms has the advantages of the organic fertilizer, the advantages of microbial manure and the advantages of the humic fertilizer, the demand of the crops on the nutrients can be maximally satisfied, and purposes of high quality and high yield can be achieved.