The invention discloses a membrane separation device capable of rotatably applying a pressure. The device comprises a water tank, supporting rods are arranged at two sides of the bottom end of the water tank, a backing plate is arranged at the bottom end of every supporting rod, a plurality of connecting rods are arranged at the bottom end of every backing plate, a water discharging tube is arranged at the middle position of the bottom end of the water tank, the top end of the water tank is provided with an opening, a hole cover matching with the opening is arranged at the top end of the opening, a matching plate is arranged at one side of the water tank, a water inlet tube is arranged at one side, away from the water tank, of the matching plate, a motor is arranged at the bottom of one side, away from the matching plate, of the water tank, a rotating shaft plate matched with the motor is arranged at one side, close to the motor, of the water tank, and a bearing is arranged in the rotating shaft plate. The water tank, the water inlet tube, the water discharging, a separation pipe, a penetration membrane 1, a penetration membrane 2 and a rotating shaft are arranged to make waters with different purifying levels selected according to the needs of personnel, so the flexibility of apparatuses is increased, water is pure, and the health of human bodies is greatly benefited.