The invention discloses a full-automatic intelligent multifunctional high-order nonlinear
fluorescence dynamics testing method and device, and relates to the field of
nonlinear optics and nano
photonics. An
optical testing device composed of an exciting light generation module, an automatic
power regulation module, an exciting
light modulation module, a sample scanning module, a
data acquisition and analysis module and an
intelligent control module is established, and under the excitation of incident
laser, based on the overall coordination of the
intelligent control module, the
optical testing device is established. Other modules are driven to complete the dynamic response test of a nonlinear
fluorescence sample, and full-automatic intelligent, multifunctional, high-precision, simple, efficient, low-cost and universal
fluorescence dynamic
test analysis is realized. The problems that traditional manual operation wastes time and labor and the testing precision is low are solved. In addition, the multifunctional integration of the
fluorescence response curve, the fluorescence
rise time, the fluorescence lifetime and the fluorescence imaging effectively improves the
test efficiency and the application scene, and greatly reduces the
system cost.