The invention discloses yoghurt melts rich in yeast beta-glucan and amino acid and a preparation method of the yoghurt melts. The raw materials of the yoghurt melts comprises the following ingredientsin parts by weight: 0.1-20 parts of skim milk powders, 0.1-20 parts of trehalose, 0.1-10 parts of whey powders, 0.1-10 parts of modified starch, 0.1-5 parts of ferment powders, 0.1-5 parts of yeast beta-glucan, 0.1-10 parts of fructo-oligosaccharide, 0.1-5 parts of monoglyceride and diglyceride fatty acid ester emulsifiers and 0.1-10 parts of acetylated starch phosphate thickeners. The preparation method comprises the following steps: weighing, mixing, preheating, homogenizing, sterilizing, cooling, inoculating, fermenting, mixing and emulsifying, congealing, traying, vacuum freeze drying, subpackaging and packaging and the like. The yoghourt melts prepared by the method have the characteristics of good taste, high stability, convenience in eating and carrying, good health-care effect andthe like, and are suitable for people of all ages such as middle-aged and elderly people, infants and the like to eat.