A cocoa butter improver (CBI) is a
specific heat-resistant cocoa butter equivalent (CBE), wherein
glycerol-1,3-distearate-2-oleate (SOS) having higher
melting point constitutes the main component in the
triglyceride composition of the CBI. In the invention, the
biosynthesis of the cocoa butter improver comprises the following steps: firstly, carrying out
transesterification reaction preferably among SOS with a high content, and
glycerol-1,2-distearate-3-oleate (SSO) and tri-
stearin (
SSS) with a low content in the presence of 1,3-specific lipases as a catalyst under the optimized conditions of the
transesterification reaction; carrying out molecular
distillation on the product obtained from the optimized
transesterification reaction to remove
fatty acid from the product, wherein the optimization is carried out by targeting on the heavy-phase yield and
acid value; and carrying out wet
fractionation on the deacidified product by using
acetone, n-
hexane and other solvents, wherein the conditions of
fractionation are optimized by targeting on the yield of SOS-rich
stearin. A large amount of
stearic acid (or low-grade
alcohol ester thereof) obtained through the removal of
fatty acid in the molecular
distillation and the palmitic components of SOO and OOO as the main
triglyceride composition obtained through the follow-up
fractionation are recyclable as the raw materials for the transesterification, thus improving the
utilization rate of raw materials. The CBI prepared through the
biosynthesis of the invention is applicable as the
raw material for the production of heat-resistant chocolate in the
food industry.