The invention discloses a burnt-out printing method for twill fabric. The method comprises the following steps: firstly, spandex fabric is prepared from spandex yarn with a twill process, then white polyester/spandex core-spun cloth is prepared from the spandex fabric and polyester yarn in a blending ratio of 50/50, the obtained white polyester/spandex core-spun cloth is treated with processes asfollows: cloth turning, end stitching, singeing, scouring, bleaching, mercerizing, polyester whitening, hot-melt coloring and setting, and a finished white cloth product to be burnt out is obtained; burnt-out pulp is added to water, a penetrating agent is added, then, plant seed gum printing paste is added, a burnt-out agent is prepared and applied to a printing machine, the finished white cloth product to be burnt out is subjected to roller printing treatment and then is baked, washed and dried and subjected to soft finish, finally, the fabric is subjected to setting, and a finished product is obtained. According to the method, the twill fabric is subjected to burnt-out processing, so that the attractive effect of the twill fabric is greatly improved, the twill fabric has better moistureabsorption property and breathability after being subjected to the burnt-out processing, and the stereoscopic effect and the texture are obviously enhanced.