The invention discloses spicy and hot beef pickled mustard tubers and a making method thereof, and belongs to the technical field of salt-pickled vegetable making. The spicy and hot beef pickled mustard tubers comprise the following main raw materials: mustard tubers, auricularia auricula, beef, chilli sauce, spices, chitosan, red wine, modified starch, edible salt and plant oil. According to the making method, the components are weighed according to a ratio, the beef which is pickled with the red wine and the modified starch is delicious and fragrant in taste, the taste of the spicy and hot beef pickled mustard tubers is enriched, and nutrient components are increased; due to addition of rich traditional Chinese herbal medicines and fragrance enhancing substances, the nutrient healthcare values of the spicy and hot beef pickled mustard tubers can be increased, and the spicy and hot beef pickled mustard tubers can be pungent, spicy, fresh and fragrant; as a natural food preservative, the chitosan is remarkable in antibacterial effect, small in use amount, good in taste and free of toxic or side effect; and the spicy and hot beef pickled mustard tubers disclosed by the invention have the characteristic of being complete in color, fragrance and taste, are fragrant, spicy and attractive, are capable of stimulating appetite, are capable of meeting requirements of people on nutrient, tasty, healthy and convenient green food, and are worthy of popularization and application.