The invention discloses a high-purity chito-oligosaccharide controlled release fertilizer and relates to the technical field of agricultural fertilizers. The high-purity chito-oligosaccharide controlled release fertilize is made from 4% of chito-oligosaccharide coated urea, 6% of another chito-oligosaccharide coated urea, urea, ammonium sulfate, calcium superphosphate, calcium magnesium phosphate, potassium sulfate, humic acid, and EDTA (ethylene diamine tetraacetic acid) chelated trace elements. The high-purity chito-oligosaccharide controlled release fertilizer has unique action on growth of crops, has the effects such as activating crop cells and promoting growth, can activate defensive reaction in plants, start anti-virus genetic expression and induce disease resistance in the crops, has the function of immunizing and killing various fungi, germs and viruses to decrease disease and pest damage to the crops, also can effectively increase the quantity and activity of soil microorganisms and improve the soil environment, is a multifunction novel fertilizer green, pollution free and soil improving, and is worthy of wide application.