The invention provides a manufacturing method of an ink-jet printing digital micro-fluidic
chip, which comprises the following steps of: 1, designing an
electrode circuit pattern to be printed, and preparing a thin film serving as a
dielectric layer; 2, according to the
electrode circuit pattern, using an ink-jet printer for carrying out ink-jet printing of nanoscale
conductive ink on one face of the
dielectric layer so as to carry out single-layer circuit printing or complex circuit printing; and 3,
coating the other surface of the
dielectric layer with a hydrophobic layer. According to the manufacturing method of the ink-jet printing digital micro-fluidic
chip, high-precision resolution ink layer control is carried out by utilizing ink jet of an ink-jet printer, the problems that steps are generated in an
electrode gap and liquid is blocked are solved, the problem of separation of an electrode and a lead is solved, and the influence of
short circuit in a complex
circuit design is effectively avoided. The ink-jet printing process is relatively simple, low in cost and high in precision, is suitable for repeated
test requirements of the digital micro-fluidic
chip in the research field, can greatly improve the working precision and efficiency of the digital micro-fluidic chip, and has practicability.