The invention discloses a preparation method of an anti-reflection film. The preparation method comprises the following steps: A, taking
silicon alkoxide, an alcohols
solvent and a
silane coupling agent to be mixed and stirred to obtain a
mixed solution A; B, taking an acid catalyst and deionized water to be mixed and stirred to obtain a
mixed solution B; C, slowly dropwise adding the
mixed solution B into the mixed solution A to obtain silica
sol; D, taking the silica
sol, hollow
silicon dioxide particles and a dispersing agent to be mixed and stirred to prepare and obtain a
coating film solution; E, selecting a glass substrate to be polished, washed and dried; F,
coating the
coating film solution on the glass substrate, leveling,
drying and curing to obtain a film layer; and G, putting the glass substrate in an alkaline solution for
corrosion, forming concave-convex fabric surface on the surface of the corroded film layer, and then washing and
drying to obtain coated glass. The interior of the anti-reflection film prepared and obtained by using the preparation method is of a hollow pore structure, and the concave-convex fabric surface is on the surface of the anti-reflection film, so that the average
reflectivity can be reduced by 2% or lower.