The invention discloses a health-keeping wine and a preparation method thereof. The health-keeping wine is prepared by adopting the following steps: according to a certain proportion, mixing the 37 raw materials, namely ginseng, radix astragali preparata, colla corii asini, angelica sinensis, honey-fried licorice root, the fruit of Chinese wolfberry, cornus officinalis, codonopsis pilosula, radix saposhnikoviae, incised notopterygium rhizome, radix angelicae pubescentis, angelica dahurica, stephania tetrandra, monkshood, gentian, scutellaria baicalensis, akebia quinata, fructus forsythia, rhizoma atractylodis, bighead atractylodes rhizome, poria cocos, pinellia ternate, bile arisaema, bamboo juice, peach kernel, flos carthami, ligusticum wallichii, radix rehmanniae preparata, pericarpium citri reticulatae, radix aucklandiae, radix clematidis, pawpaw, achyranthes bidentata, stone-like omphalia, platycladi seed, red yeast rice and brown sugar; then, soaking the mixture with white spirit or rice wine, so as to obtain the health-keeping wine. The health-keeping wine has the effects of nourishing qi, soothing the nerves, replenishing blood, activating blood, invigorating spleen, supplementing qi, clearing heat, relieving exterior syndrome, enhancing the immunity, preventing bacteria, preventing viruses, improving eyesight, relieving thirsty, beautifying, and delaying senescence; through the effect of replenishing blood and activating blood, the balance between yin and yang of the five internal organs can be conditioned, the blood supply of the heart and brain can be improved, the smoothness of qi and blood can be facilitated, and the memory can be improved, and the health-keeping wine can prevent fatigue, tonify deficiency, enhance physique, and improve the immunity.