The invention discloses a preparation method of pollution-free edible children plasticine, belonging to the field of preparation of children toys. The preparation method comprises the following steps: washing polished round-grained rice and sticky rice to be clean, mixing the washed polished round-grained rice and sticky rice, soaking the mixed rice by adopting fresh milk after lactobacillus fermentation, so that the ageing resistance of the product is improved, carrying out ball-milling on the soaking solution and the soaked rice to obtain rice milk, carrying out microwave heating gelatinization, carrying out homogenizing treatment, regulating the pH till the pH is neutral, carrying out centrifugal dewatering, carrying out drying, and mixing the dry matter with beewax, glycerinum and the like at a certain ratio, thus obtaining the pollution-free edible children plasticine. According to the preparation method, the adopted raw materials are pollution-free non-toxic materials, so that the obtained plasticine is stable in chemical property, free of toxic and side effects and good in plasticity, can be repeatedly used without deterioration, and can be digested for absorption by the digestive system after being put into the mouth, and no adverse effects are caused to the growth and development of children.