37 results about "Bisulfite salt" patented technology

The invention belongs to the technical field of fine chemical organic synthesis, and discloses a synthesis method of 1, 3-propane sultone without generation of three wastes. The synthesis method comprises the following steps: by taking allyl alcohol, sulfite and hydrosulfite as raw materials, generating 3-hydroxy propanesulfonate; then removing part of water, adding a solvent A to separate out sulfite, filtering, and adding sulfuric acid into filtrate for acidification; and adding a solvent B to separate out sulfate, filtering and separating out the sulfate, and adding a dehydrating agent to carry out dehydration cyclization reaction to prepare the 1, 3-propane sultone. According to the synthesis method provided by the invention, a 1, 3-propane sultone product with the yield not lower than 95% and the purity reaching 99.9% or above and a sulfate byproduct with the purity reaching 99.6% or above can be obtained; no waste gas, waste solid or waste water is generated in the reaction process, the catalyst sulfite can be repeatedly used, and the used solvent is easy to recover and can be repeatedly used.

The invention discloses a tetrahydrocarbazole enhanced fluorescent probe for detecting hydrosulfite as well as a preparation method and application of the tetrahydrocarbazole enhanced fluorescent probe. The fluorescent probe is a 2-(2-(5-(2, 2-dimethyl-2, 3, 4, 9-tetrahydro-1H-1, 3-methyl carbazole-6-yl) furan-2-yl) vinyl)-3-ethyl benzothiazole-3-onium iodised salt compound, namely, TC-FV-BT, and the fluorescent probe can be applied to the field of fluorescence detection. The probe TC-FV-BT can be used for specifically recognizing the hydrosulfite. Under sunlight, after bisulfite is added into a DMF / H2O (2: 8, v / v) solution of the probe TC-FV-BT, the color of the solution is changed from dark pink to white, under irradiation of ultraviolet light with the wavelength of 365 nm, the solution emits orange fluorescence at the emission wavelength of 605 nm, the fluorescence color of the solution is changed from colorless to orange, the response time is 4 min, the detection limit of the bisulfite reaches 77.27 nM, and the detection limit of the bisulfite reaches 77.27 nM. The fluorescent probe shows good selectivity and anti-interference performance, and has a good application prospect in the fields of environmental analysis, food and the like as a fluorescent probe for detecting hydrosulfite.

The invention belongs to the technical field of biological detection, and particularly relates to a bisulfite-free detection kit for specific site 5-methylcytosine and a detection method thereof. According to the method, a TAPbeta treatment method is adopted, 5hmC is sealed through beta-GT, 5mC oxidation is carried out through TET, and 5mC is reduced into dihydrouracil (DHU) through pyridine borane, so that the apparent difference between 5mC and C and 5hmC is converted into single nucleotide polymorphism difference. In addition, a flow microsphere technology based on PNA-assisted click chemical connection starting is established to detect DNA methylation specific sites. A PNA clip is innovatively introduced into click chemical connection, and non-specific connection is inhibited. Target sequence enrichment, signal amplification and signal acquisition are carried out through surface-functionalized magnetic nanoparticles, and the detection sensitivity is improved to fM. The invention provides a new tool for high-specificity and high-sensitivity epigenetic marker detection.

The invention provides a reverse osmosis reducing agent and a preparation method and application thereof, and belongs to the technical field of reverse osmosis water treatment.The reverse osmosis reducing agent comprises a first reducing agent, a second reducing agent and a solvent; the first reducing agent is thiosulfate, and the second reducing agent is sulfite and / or bisulfite; the mass ratio of the first reducing agent to the second reducing agent is (1: 18)-(10: 1). The reverse osmosis reducing agent can be applied to reverse osmosis water treatment, the reverse osmosis reducing agent is added into reverse osmosis inlet water to be treated, and the reverse osmosis inlet water subjected to reduction treatment can be obtained after full reaction. The first reducing agent and the second reducing agent are compounded according to the specific mass ratio, so that the compounded reverse osmosis reducing agent can effectively inhibit the occurrence of advanced oxidation chain reaction while keeping high reducibility, and effective reduction of reverse osmosis inlet water can be realized by using a small amount of the reverse osmosis reducing agent.

The present application relates to a method and system for inferring the age of an individual from semen or a semen stain, and a methylation detection kit, which comprises the following steps: firstly, extracting the genomic DNA of the semen or semen stain of the individual; secondly, performing bisulfite treatment on the genomic DNA; thirdly, selecting a plurality of sites in the CpG sites in the genomic DNA and amplifying the selected CpG sites to obtain the methylation rate of the selected CpG sites; and finally, performing regression analysis on the methylation rate of the selected CpG sites and the age of the individual to construct a regression model for inferring the age of an unknown individual from semen or a semen stain. The average absolute error of the present application reaches 1.68 to 4.44 years.

Described herein are methods of preparing dual-indexed nucleic acid libraries for methylation profiling using bisulfite conversion sequencing. In various embodiments, the methods use a two-step indexing process to tag bisulfite-treated DNA with unique molecular identifiers (UMIs).

The invention relates to the technical field of cosmetics, in particular to a method for improving the stability of a sialic acid-p-hydroxyacetophenone solution. The method comprises the following steps: mixing p-hydroxyacetophenone with a stabilizer, and then mixing with sialic acid to obtain sialic acid-p-hydroxyacetophenone mixed liquor; the stabilizer is sulfite and / or hydrosulfite, and the addition amount of the stabilizer is 0.005%-0.2% by mass. Aiming at the problems of discoloration of sialic acid-p-hydroxyacetophenone solution and loss of sialic acid, the invention provides a method, all the raw materials are mixed according to a specific sequence on the basis of a specific stabilizer through a specific processing technology, the stability of the obtained solution is remarkably improved, the solution does not discolorate after being placed for a long time, the loss rate of sialic acid is low, and the method is suitable for industrial production. The method has important application value in the field of production of cosmetics containing sialic acid at the same time.

The methods, compositions, and kits of the disclosure provide a novel approach for a whole genome, unbiased DNA analysis method that can be performed on limited amounts of DNA can be used to analyze DNA to determine its modification status. Aspects of the disclosure relate to a method for amplifying bisulfite-treated deoxyribonucleic acid (DNA) molecules comprising: (a) ligating an adaptor to the DNA molecules, wherein the adaptor comprises a RNA polymerase promoter comprising bisulfite-protected cytosines; (b) treating the ligated DNA molecules with bisulfite; (c) hybridizing the bisulfite-treated DNA molecules with a primer; (d) extending the hybridized primer to make double stranded DNA; and (e) in vitro transcribing the double-stranded DNA to make RNA.

The invention discloses an LAMP (loop-mediated isothermal amplification)-based rapid detection primer composition and kit for non-muscle invasive bladder cancer and application of the LAMP-based rapid detection primer composition and kit. The primer composition comprises a primer group for targeted amplification of a VIM gene and a TMEFF2 gene. According to the method, rapid nucleic acid extraction is achieved through a one-step method reagent, enzymatic methylation conversion is combined to replace a traditional bisulfite method, and nucleic acid damage and detection complexity can be remarkably reduced. A double-target detection system is constructed on the basis of VIM and TMEFF2 genes, and visual rapid detection of the non-muscle invasive bladder cancer is realized in combination with an LAMP detection technology. The whole detection process does not need a centrifugal machine or a PCR instrument, extraction, conversion and detection can be completed only through a metal bath or a water bath kettle, operation is easy and convenient, and cost is low. The kit provided by the invention is suitable for household self-test, reduces the detection burden of patients, and has wide clinical and household application prospects.

The application is a method for adsorbing and removing low-concentration sulfur dioxide and dust in flue gas, which relates to the use of macroporous weak acid cation exchange resin which is transformed into weak base resin to adsorb and remove sulfur dioxide. The specific steps are as follows: after the flue gas is cooled and humidified by water washing, it is introduced into an adsorption column filled with macroporous weak acid cation exchange resin. The resin is packed in the form of bundled bags or scattered packing, or a combination of the two. During the adsorption process, the resin particles slowly shrink and discharge bisulfite solution, which can be used as a by-product or discharged harmlessly after oxidation. In addition, the resin layer can also intercept and capture small particles of dust in the flue gas, achieving the effect of dust removal. The resin can be regenerated by alkali leaching. The process of the application is simple, the desulfurization and dust removal efficiency is high, the equipment investment is small, and it has universality, which is suitable for the desulfurization needs of multiple industrial fields, especially for the capture and removal of low-concentration SO2 generated by small and medium-sized emission sources.

The present application belongs to the field of medicine and chemical industry, and relates to a method for purifying losartan. In the method, bisulfite or sulfite is used to remove color impurities of losartan, and the method has the advantages of mild treatment condition, short operation period, high decolorization efficiency and suitability for industrial production.

The methods, compositions, and kits of the disclosure provide a novel approach for a whole genome, unbiased DNA analysis method that can be performed on limited amounts of DNA can be used to analyze DNA to determine its modification status. Aspects of the disclosure relate to a method for amplifying bisulfite-treated deoxyribonucleic acid (DNA) molecules comprising: (a) ligating an adaptor to the DNA molecules, wherein the adaptor comprises a RNA polymerase promoter comprising bisulfite-protected cytosines; (b) treating the ligated DNA molecules with bisulfite; (c) hybridizing the bisulfite-treated DNA molecules with a primer; (d) extending the hybridized primer to make double stranded DNA; and (e) in vitro transcribing the double-stranded DNA to make RNA.

The present invention relates to a stabilized composition with significantly improved shelf-life stability. More specifically, the present invention relates to a stable liquid aqueous pharmaceutical composition comprising cyticine or a pharmaceutically acceptable salt thereof, at least one antioxidant selected from the group consisting of sulfite compounds, bisulfite compounds, and pyrosulfite compounds. The present invention also relates to the use of at least one antioxidant selected from the group consisting of sulfite compounds, bisulfite compounds, and pyrosulfite compounds for stabilizing a liquid aqueous pharmaceutical composition comprising cyticine.

The application discloses a solution containing natural macromolecule and a coagulation molding method. The fiber stream obtained by wet spinning of the solution containing natural macromolecule is coagulated and molded through at least one coagulation bath. The solution containing natural macromolecule contains metal hydroxide and an additive selected from at least one of urea, zinc oxide and thiourea. Bicarbonate or bisulfite or a combination of bicarbonate and carbonate or a combination of bisulfite and sulfite is used as a component of the coagulation bath. The low-cost, green, efficient, non-toxic, harmless and inexpensive inorganic salt solution does not contain strong acid and organic solvent, and can be used to prepare long fibers with circular cross section and excellent mechanical properties. The long fibers have smooth surface, softness and luster. In the coagulation bath, the bicarbonate and carbonate or the bisulfite and sulfite can be easily separated and recovered.

The methods, compositions, and kits of the present disclosure provide a new method for whole genome, i.e., unbiased DNA analysis, that can be performed on limited amounts of DNA. It can be used to analyze DNA to determine its modification status. Aspects of the present disclosure relate to methods for amplifying a bisulfite treated deoxyribonucleic acid (DNA) molecule, the method comprising: (a) ligating an adaptor to the DNA molecule, wherein the adaptor comprises a RNA polymerase promoter comprising bisulfite protected cytosines; (b) treating the ligated DNA molecule with bisulfite; (c) hybridizing the bisulfite treated DNA molecule to a primer; (d) extending the hybridized primer to produce double stranded DNA; and (e) in vitro transcribing the double stranded DNA to produce RNA.

The invention provides high-quality dissolving pulp and a preparation method thereof, and belongs to the technical field of dissolving pulp, the preparation method comprises the following steps: S1, loading wood flour into a spherical digester, adding a sodium hydroxide solution, controlling temperature and pressure, preserving heat and cooking to obtain coarse pulp; s2, deionized water is added into the crude pulp, the pH is adjusted, xylanase is added, then an activating auxiliary agent is added, activating treatment is conducted, pretreated pulp is obtained, and the activating auxiliary agent is a mixture of hydrosulfite and citric acid; s3, a chelating agent is added into the pretreated slurry, purified slurry is obtained, and the chelating agent is sodium tripolyphosphate cross-linked modified chitosan; and S4, carrying out bleaching treatment on the purified pulp by adopting chlorine dioxide, and obtaining the high-quality dissolving pulp after the bleaching is completed. According to the high-quality dissolving pulp prepared by the method, the alpha cellulose content can reach 94.2% or above, the iron content is 20 ppm or below, and the whiteness is 85% or above.

This invention discloses a method for detecting 5-hydroxymethylcytosine based on triple cascade signal amplification, belonging to the field of biochemical detection technology. The method includes the following steps: 1) preparation of 5hmC-specific glycosylation and azide labeling; 2) magnetic bead capture and enrichment separation of target DNA; 3) TdT-mediated nucleic acid end polymerization and extension; 4) APE1-triggered primer release and rolling circle amplification; 5) DNase-catalyzed cleavage and fluorescence signal detection. This invention is bisulfite-free and sequence-independent, achieving extremely low background and ultra-high sensitivity detection with a detection limit of 4.41 fM. It can be widely applied to early clinical cancer screening, biomarker detection for neurological or cardiovascular diseases, and provides a powerful platform for low-abundance epigenetic analysis in clinical diagnosis and biomedical research.

The invention discloses a stomach cancer early screening ELISA kit and an early screening detection method thereof, and relates to the technical field of in vitro diagnosis. The kit comprises the following components: a pre-coated 96-well plate which is fixedly provided with methylated DNA fragments of an anti-5-methylcytosine monoclonal antibody (with the clone number of 33D3), cg07160992, cg13516541, cg02956093 and cg21496913; the detection assembly comprises a biotinylation detection probe and a streptavidin-HRP (horse radish peroxidase) conjugate; the pretreatment assembly comprises a hydrosulfite conversion reagent and MBD2 protein modified magnetic beads. According to the kit, through a double-index combined interpretation model, the sensitivity to early gastric cancer reaches 86%, the blank of non-invasive detection of stage I gastric cancer is filled, the total sensitivity is 90.5%, the international top-level cancer early screening product standard is met, operation is standardized, special instruments are not needed, and the kit has high clinical application value.

Compounds exhibiting antiviral activity and / or inhibition of viral replication against viruses, particularly those belonging to the picornavirus-like supercluster, including coronavirus having a formula: (I) where X comprises a cyclic moiety, R2 is a branched or unbranched alkyl, cycloalkyl, aryl, arylalkyl, alkenyl, alkynyl, amino acid side chain, bicyclic or tricyclic side chain, combinations, and substituted forms thereof, and Z is selected from the group consisting of C1-C6 hydroxyalkyl, aldehydes, alpha-ketoamides, and bisulfite salts, and in particular —CH2OH, —CHO, —CH(0H)S03−Na+, and -[0(C=0)Rw]S03−Na+.

The invention discloses a method and a kit for testing the methylation level of a CpG site, and relates to the technical field of biomedical detection. The CpG site methylation level testing method comprises the following steps: carrying out hydrosulfite conversion treatment on a DNA sample containing a target CpG site, and then carrying out RPA amplification reaction to obtain a target solution; performing a first guide RNA mediated nuclease detection reaction on one part of the target solution, and performing a second guide RNA mediated nuclease detection reaction on the other part of the target solution to respectively obtain a first detection signal and a second detection signal; the methylation level of the target CpG site is obtained by calculating the proportional relation between the first detection signals. The method is based on an endogenous signal normalization mechanism, has the characteristics of rapidness, accuracy, stability and economy, and can fundamentally eliminate quantitative deviation caused by difference between sample concentration and amplification reaction efficiency.

Compounds that exhibit antiviral activity and / or inhibit viral replication against viruses, in particular viruses belonging to the picornavirus-like supergroup, including coronaviruses, having the following formula: (I) wherein X comprises a cyclic moiety, R2 is a branched or unbranched alkyl, cycloalkyl, aryl, arylalkyl, alkenyl, alkynyl, amino acid side chain, bicyclic or tricyclic side chain, combinations and substituted forms thereof, and Z is selected from the group consisting of C1-C6 hydroxyalkyl, aldehyde, alpha-ketoamide, and bisulfite, and in particular -CH2OH, -CHO, -CH(OH)SO3 ‑ Na + , and -[0(C=0)R w ]SO3 ‑ Na + .

The technology provided herein relates to amplification-based detection of bisulfite treated DNA, and in particular, but not exclusively, to methods and compositions for multiplex amplification of low levels of sample DNA prior to further characterization of the sample DNA. The technology also provides methods for isolating DNA from a blood or blood product sample, such as a plasma sample.

The invention provides a composition and application of the composition in conversion of DNA (deoxyribonucleic acid) from hydrosulfite, and belongs to the technical field of biology. The oyster glycogen is added into the conversion liquid, so that the loss of DNA in the hydrosulphite conversion and recovery process can be effectively reduced, the recovery efficiency of DNA is improved, and hydrosulphite conversion and methylation detection of low-input DNA are facilitated.

Provided herein is technology relating to the amplification-based detection of bisulfite-treated DNAs and particularly, but not exclusively, to methods and compositions for multiplex amplification of low-level sample DNA prior to further characterization of the sample DNA. The technology further provides methods for isolating DNA from blood or blood product samples, e.g., plasma samples.

The present application relates to the field of biotechnology and molecular diagnosis, and particularly relates to a methylation detection pretreatment kit and method for protecting DNA integrity, which can solve the problem that DNA degradation is serious in traditional bisulfite conversion technology, and conversion efficiency and DNA integrity are difficult to be obtained simultaneously, the kit comprises a conversion solution, a protection solution, a combination solution, a desulfonation solution, a washing solution and an elution solution, the conversion solution contains 2.5-4 mol / L bisulfite and linear polyethylene polyamine, the protection solution contains an organic denaturant and a polyphenol antioxidant, the DNA melting temperature is reduced by the organic denaturant, the linear polyethylene polyamine accelerates the conversion reaction, and the desulfonation system with low water activity is combined to complete the bisulfite conversion under mild conditions of 50-70 DEG C; the present application can realize the conversion efficiency of greater than 99.5% of unmethylated cytosine, greatly reduce DNA degradation, significantly improve the long fragment DNA recovery rate and the detection sensitivity of trace samples, and is suitable for the methylation detection of three generations of long read sequencing and clinical trace samples.

The invention discloses a DNA (deoxyribonucleic acid) gel dish desalting treatment method and application, which is characterized in that a DNA solution is dialyzed and replaced through a natural molecular sieve of gel by utilizing the molecular sieve effect formed by agarose gel polymerization and combining the characteristic that DNA is a long-chain macromolecule, so that the DNA treated by sodium bisulfite is desalted and purified. Meanwhile, the molecular sieve of the gel is used for dialyzing the desalted solution, the tedious process of removing hydrosulphite and re-purifying DNA in DNA methylation sequencing is innovatively solved, the treated long-chain DNA is wrapped in agarose gel, the loss or loss of DNA in the dialysis process is small, an expensive purification reagent and a purification step with tedious operation are not needed, and the method is suitable for large-scale industrial production. The experiment cost and the operation process can be greatly saved.

The present invention discloses a pinanyl-based open-type fluorescent probe for detecting bisulfite, and its preparation method and application. The probe is 3-(2-(2-methoxy-7,7-dimethyl-5,6,7,8-tetrahydro-6,8-methylenequinazoline-4-yl)vinyl)-1-methylquinolin-1-ium trifluoromethanesulfonate (TQZQ-TfO). The present invention uses 7,7-dimethyl-4-(2-(quinoline-3-yl)vinyl)-5,6,7,8-tetrahydro-6,8-methylenequinazoline-2-ol (abbreviated as TQZQ) as raw material, and undergoes quaternization reaction with methyl trifluoromethanesulfonate to prepare TQZQ-TfO. The compound can undergo a specific addition reaction with bisulfite. Under ultraviolet light with a wavelength of 365nm, the solution of TQZQ-TfO has no fluorescence. After adding bisulfite, the solution emits strong yellow fluorescence. Therefore, TQZQ‑TfO can be used as an open-type fluorescent probe for the detection of bisulfite, with many advantages such as good selectivity, high sensitivity (low detection limit of 12 nM), fast response speed (within 5 s), and a wide pH range (4‑12), showing good application prospects.

The invention provides a method for detecting pseudouracil based on rolling circle amplification mononucleotide resolution and application, and belongs to the technical field of nucleic acid chemical modification detection. The method comprises the following three steps: hydrosulfite treatment, ligase-mediated locked probe cyclization and RCA. By converting a bisulfite-derived psi signal into a lock-type probe connection gate, single nucleotide resolution detection can be realized without qPCR, and the RNA input amount is low. According to the method, psi in rRNA and mt-tRNA can be qualitatively identified, absolute quantification can be carried out on target RNA, and meanwhile the stoichiometric ratio of site-specific psi is measured. In peripheral blood, the method enables the differentiation of samples from AML patients and healthy controls, supporting the use of site-specific psi measurements as candidate biomarkers; and in combination with a GLORI-mediated deamination reaction, the method can be suitable for modification of other RNA (Ribonucleic Acid). The method has potential promotion function research and clinical transformation research.