8 results about "Botfly" patented technology

A rapid detection and visualization method for triple RPA-LFA targeting necrophilic flies includes the following steps: Step 1: DNA amplification reaction; DNA is extracted from *Gynostemma pentaphyllum*, *Gynostemma spp.*, *Variegata spp.*, *Variegata spp.*, *Bulbus rubrothes*, *Gynostemma pentaphyllum*, *Flyflies fasciatus*, and *Heterodon spp.* using the SteadyPure Universal Genomic DNA Extraction Kit; the concentration and purity of the genomic DNA of the above species are assessed using a Nano Drop 2000 spectrophotometer; the reaction is performed according to the instructions of the DNA isothermal rapid amplification kit; the reaction system uses Buffer A, a primer mixture consisting of 3 pairs of primers; the mixture is incubated in a metal bath to perform the RPA reaction and obtain the amplified product; Step 2: Visual detection; Mixed amplification product: the amplified product is mixed with dd water to obtain a mixed amplification product; the mixed amplification product is dropped onto the LFD test strip sample pad, and then the result is displayed. This invention features no dependence on a sequencer, simple operation, rapid detection, and visualization.

Methods are provided for insect control, including controlling fly maggot disease, in an animal by securing to the animal an elastic band impregnated or containing therein an insecticide, an insect repellant, lidocaine, or any combination thereof. The insect control may be dual mode control, and may be directed against existing or impending open wounds, including open wounds caused by castration, tagging, umbilical ligation, or chamfering of animals. Also provided are methods for castration, tail cutting, umbilical cord ligation, or chamfering of animals while insect control. Elastic bands for insect control and methods of making such elastic bands are also provided.

The utility model relates to the technical field of fly maggot breeding, in particular to a fly maggot breeding assembly line. The device comprises a separating plate and a support, the separating plate is in sliding connection with the surface of the support, a sliding groove is formed in one side of the support, a collecting device is arranged on the surface of the sliding groove of the support, the collecting device comprises a collecting bin, and the collecting bin is in sliding connection with the surface of the sliding groove of the support. Two connecting rods are fixedly connected to the surface of the collecting bin, a grip is fixedly connected to one end of each connecting rod, a connecting hole is formed in the surface of the support, a fixing rod is fixedly connected to one side of the support, and a fixing plate is rotatably connected to the end, away from the support, of the fixing rod. The fly maggot collecting device solves the problems that the separated fly maggots can be scattered everywhere and climb everywhere, so that the working environment becomes dirty, disordered and bad, workers have to manually collect the fly maggots scattered everywhere, and the working intensity of the workers is increased.

There is provided methods for insect control, including controlling myiasis, in animals by affixing to the animal an elastomeric band having infused or contained within an insecticide, an insect repellent, lidocaine, or any combination thereof. The insect control may be a dual-mode control and may be in respect of existing or impending open wounds, including open wounds caused by castration, tail docking, umbilical cord ligation, or dehorning of an animal. There is also provided methods for castration, docking, umbilical cord ligation, or dehorning of an animal with concurrent insect control. Also provided are elastomeric bands for insect control and methods for preparing such elastomeric bands.

The application discloses a medicine injection method for killing camel head fly larvae parasitizing in the nasal cavity of a bactrian camel. A pair of non-bony structures similar to a triangle and the size of a thumb are arranged at the joint of nasal bone, maxillary bone and frontal bone. The pair of non-bony structures are at the same horizontal level with the eye socket of the bactrian camel, are located at the intersection of the inner corner of the eye and the upper edge of the eye socket, and are away from the eye socket by 4-5 cm. After a syringe is vertically inserted into 2-3 cm at the site, the needle of the syringe can reach the medial part of the concha nasalis. After the site is inserted into 2.5-3 cm in the clockwise direction and is inclined by 45 degrees, the needle can reach the lateral part of the concha nasalis. The medicine injection method can directly inject the insect killing medicine to the parasitizing position of the first instar larvae, and the purpose of killing the first instar larvae is achieved. The medicine injection method provides a new method for killing the first instar larvae in the overwintering period, and can reduce the economic loss of the camel raising industry caused by the nasal fly maggot disease.

The invention belongs to the field of fly maggot breeding, and provides a method for integrally breeding fly maggots by using pig manure in Tibet, which comprises the following sequential steps: hatching period: placing red-head fly pupae in a constant-temperature and constant-humidity environment for eclosion, controlling the environment temperature to be 25-30 DEG C and the relative humidity to be 50-70%, and immediately providing feed and clean drinking water after the pupae are subjected to eclosion into adult flies; by constructing a pig manure-fly maggot-protein feed-organic fertilizer integrated breeding mode, the double problems that in Tibetan pig breeding in the Tibetan alpine region, the manure treatment cost is high, transportation is difficult, the resource utilization rate is low, and protein feed depends on outsourcing for a long time are effectively solved; and in combination with self-circulation maintenance of red-head fly populations introduced in Sichuan, the egg laying rate and egg hatching rate of adult flies are increased, and the inhibition effect of plateau low-temperature oxygen-deficient environment on fly maggot growth is overcome.

The invention discloses a bio-organic fertilizer based on fly maggot chitin and a preparation method of the bio-organic fertilizer, the fly maggot chitin is extracted by an enzyme-alkali synergistic method to generate chitosan oligosaccharide, and the efficient and environment-friendly bio-organic fertilizer is prepared by combining organic materials and complex microbial inoculants. The fertilizer can significantly increase the crop yield (increase the yield by 20-100%), improve the soil structure (the organic matter is greater than or equal to 65%), and inhibit soil-borne diseases (the morbidity of root-knot nematode is reduced by 95%). The method provided by the invention realizes resource utilization of wastes, and has significant economic and environmental benefits.

This utility model provides a pelleting device for fly larvae biological feed, belonging to the technical field of pelleting devices. It includes a base, a support fixedly connected to the surface of the base, a vertical plate fixedly connected to the surface of the support, a material bin fixedly connected to the surface of the vertical plate, and a hydraulic cylinder fixedly connected to the surface of the support. This utility model incorporates a cylinder, a connecting plate, a moving plate, a fixed frame, a cutting plate, and a scraper. The cylinder drives the connecting plate and the moving plate to first layer the material bin. Then, the raw material is fed into the hydraulic cylinder, which pushes the extrusion plate to extrude the raw material, squeezing out excess moisture and reducing the difficulty of forming and adhesion during subsequent extrusion molding. A motor B drives the scraper to remove surface residue from the cutting plate, effectively reducing pellet adhesion and facilitating subsequent cleaning.