75 results about "Cyanobacterin" patented technology

An IBTL system having a low GHG footprint for converting biomass to liquid fuels in which a biomass feed is converted to liquids by direct liquefaction and the liquids are upgraded to produce premium fuels. Biomass residues from the direct liquefaction, and optionally additional biomass is pyrolyzed to produce structured biochar, hydrogen for the liquefaction and upgrading, and CO₂ for conversion to algae, including blue green algae (cyanobacteria) in a photobioreactor (PBR). Produced algae and diazotrophic microorganisms are used to produce a biofertilizer that also contains structured biochar. The structured biochar acts as a nucleation agent for the algae in the PBR, as a absorption agent to absorb inorganics from the biomass feed to direct liquefaction or from the liquids produced thereby, and as a water retention agent in the biofertilizer. The ratio of cyanobacteria to diazotrophic microorganisms in the biofertilizer can be selected to optimize the so as to achieve desired total chemically active carbon and nitrogen contents in the soil for a given crop.

The present invention provides novel photobioreactors, modules thereof, and methods for use in culturing and harvesting algae and cyanobacteria.

Disclosed is a modified photoautotrophic bacterium comprising genes of interest that are modified in terms of their expression and/or coding region sequence, wherein modification of the genes of interest increases production of a desired product in the bacterium relative to the amount of the desired product production in a photoautotrophic bacterium that is not modified with respect to the genes of interest.

A photobioreactor system and the methods of using the system for cultivating photosynthetic organisms, including algae, cyanobacteria, plants, and plant cells. A single photobioreactor unit consists of transparent materials (such as glass, fiberglass, or plastics) and non-transparent materials (such as stainless steel or other metals). Two or more photobioreactor units can be connected with each other in a cascade to form a photobioreactor system, which can be used for cultivation of the photosynthetic organisms.

The invention relates to the fields of gene engineering and biosynthesis, and concretely relates to a construction body for efficiently synthesizing ethylene, and a construction method and an application thereof. The construction body comprises an active promoter and an ethylene synthetase gene (efe) controlled by the promoter. Carbon dioxide can be immobilized by above engineering bacterium through using solar energy in order to synthesize ethylene. An ethylene synthesis approach is constructed in cyanobacterial cells in order to realize immobilization of carbon dioxide in photosynthetic microbal cyanobacterial cells through using solar energy and synthesis of ethylene, wherein energy for ethylene synthesis is from the solar energy, and a carbon source is carbon dioxide. The method can be used to prepare bioethylene without restriction of petroleum shortage, and the bioethylene does not increase carbon emission.

The invention discloses a new function of cyanobacteria naturally existing in nature, and aims to provide a photosensitizer with a simple preparation process, overcome a tumor hypoxia microenvironmentthrough oxygen self-production by utilizing the characteristic that cyanobacteria generates oxygen through photosynthesis after being illuminated, and target tumors to play an effective photodynamicrole. The in-vitro experiments prove that the cyanobacteria can effectively inhibit the growth of 4T1 cells after being irradiated by laser with a wavelength of 660nm. The photosensitizer is simple inprocess step and low in cost, only a large amount of cyanobacteria needs to be simply multiplied and cultured, and oxygen can be supplied to the photosensitizer by providing specific illumination, sothat tumor hypoxia is overcome, the photodynamic therapy effect is achieved, and the photosensitizer capable of generating oxygen by itself and having industrial practical value is developed.

Described herein are cyanobacteria encapsulated in hydrogels. Also described herein are consortia that include encapsulated cyanobacteria and heterotrophic microbes. The encapsulated cyanobacteria can be autotrophic and can provide nutrients to the heterotrophic microbes, which can utilize the nutrients to grow and produce useful products.

The invention provides a cyanobacterium of a living drug. Oxygen is generated by means of photosynthesis under laser radiation to improve tumor hypoxia, so that the tumor microenvironment is changed,an oxygen-increasing tumor therapeutic effect is achieved, and novel selection is provided for cancer treatment. An in vitro experiment verifies that the cyanobacterium can generate oxygen infinitelyunder a condition of 660 nm laser radiation. An in vivo experiment verifies that the tumor hypoxia condition is improved obviously by tail vein injection of the cyanobacterium into a mouse body and radiation on mouse tumors by 660 nm laser.

The invention discloses a green fluorescent cyanobacterium phytochrome fluorescence indicator preparation method. A fusion protein comprises a second GAF structural domain of cyanobacterium phytochrome protein all3691 and streptavidin. A sequence of the fusion protein can be easily and stably expressed in microorganisms, and defects of difficulty in acquisition of high-purity natural phycobiliprotein and cyanobacterium phytochrome, high preparation cost, chemical modifier consumption, polymerization morphological instability of natural phycobiliprotein and the like are avoided. The expressionmethod is free of phycobiliprotein lyase participation, transformational gene amount is decreased, and strain stability and screening efficiency are improved. In addition, by optimization of fermentation media and fermentation conditions, fusion protein yield is greatly increased.

The invention discloses a saline-alkali soil heavy metal ion remover and a preparation method thereof. The saline-alkali soil heavy metal ion remover comprises the following raw material components byweight: 30-45 parts of mineral source humus ultrafine powder, 5-10 parts of a phanerochaete chrysosporium extracellular polymeric substance, 4-8 parts of cyanobacteria liquid, 15-20 parts of lignin grafted polyacrylonitrile, 5-10 parts of citric acid, 20-25 parts of straw powder, and 15-20 parts of desulfurized gypsum. The mineral source humus ultrafine powder in the soil desalting agent can improve the air permeability and water permeability of soil and strongly adsorb various heavy metal ions, also has strong water retention and storage capacity, and improves the landform of saline-alkali soil, so that the saline-alkali soil can adapt to more and more plant growth, and the selectivity of agricultural planting is improved.

The invention discloses a microbial fertilizer for promoting decomposition of soil organic matters. The microbial fertilizer comprises diatomite, bamboo powder, lentinan, yeast extract, vitamin C powder, lignin grafted polyacrylonitrile, microbial agent, cyanobacteria liquid and trace elements, wherein the microbial agent comprises bacillus thuringiensis, bacillus subtilis, bacillus megaterium, bacillus mucilaginosus, bacillus laterosporus, streptomyces jingyangensis and aspergillus niger. Tests prove that the microbial fertilizer in the embodiment of the invention can effectively increase theyield of bitter gourds planted in saline-alkali soil; and moreover, the microbial fertilizer can effectively reduce the pH of soil and the salt content and organic matter content in the soil. The preparation method of the microbial fertilizer is simple, related microbial strains are also common strains on the market, the use method is simple, the microbial fertilizer does not conflict with conventional fertilization, the yield increasing effect and the soil rational property improving effect are obvious, and the microbial fertilizer is suggested to be popularized and used in saline-alkali soil areas.

A method for obtaining biofertilizer concentrates and biostimulants for agricultural use from biomass of microalgae, including cyanobacteria, wherein, firstly, the cellular content of the microalgae, including cyanobacteria, is released by rupturing the cells thereof; next, the protein fraction and the cell wall of the microalgae, including cyanobacteria, obtained from said release of the cellular content, are hydrolyzed with proteolytic enzymes until producing free amino acids; next, nitrogen (N), phosphorus (P) and/or potassium (K) excipients are added to the previously obtained product of enzymatic hydrolysis.

The invention discloses a method for treating cyanobacteria green plant sewage plant and kitchen waste through cooperation of photoelectricity and heat, and belongs to the technical field of environmental protection and renewable energy source treatment. After high-temperature pyrohydrolysis/high-temperature disinfection and sterilization, municipal sewage plant organic sludge, municipal organic kitchen waste, municipal other organic matter waste, blue algae and other waste are subjected to high-temperature anaerobic digestion, biogas residue dehydration, high-pressure squeezing and solar drying, finally, resource utilization is carried out, biogas is generated during high-temperature anaerobic digestion treatment, and the biogas is conveyed into a biogas storage cabinet and is collected.Resourceful treatment and the comprehensive treatment process of direct squeezing of a biogas residue dehydrator dehydration high-pressure filter press and solar drying are fully realized, stabilization, harmlessness and reduction of blue-green algae (blue-green algae and blue bacteria), urban green plant waste, sewage plant sludge and kitchen waste can be achieved, meanwhile, waste is turned intowealth, and available biogas and fertilizer are produced.

The invention discloses an inhibitor for inhibiting ammonia dissipation of cow dung in a cowshed. The inhibitor is prepared from 35-45 parts of plant extract, 25-30 parts of bacillus, 15-20 parts of actinomycetes, 8-10 parts of lactic acid bacteria, 6-8 parts of cyanobacteria, 4-8 parts of yeast powder, 2-4 parts of bacillus aceticus, 10-12 parts of a nitrifying bacteria culture medium, 20-25 parts of alums, 20-25 parts of monopotassium phosphate and 400-600 parts of water. The method for inhibiting ammonia dissipation of cow dung in the cowshed comprises the following steps: gathering the cow dung scattered in the cowshed, diluting the solution obtained in the previous step according to the ratio of 1: 150, filling the diluted solution into a spraying device, spraying the cow dung every 6-7 days for 3-5 times per day, and stirring the cow dung during spraying.

The invention discloses a ground source heat pump synergistic cyanobacteria green plant and sewage plant and kitchen waste treatment method, and belongs to the technical field of environmental protection and renewable energy source treatment. Biogas in the biogas storage cabinet is desulfurized, purified and extracted to provide power for a state grid and a municipal gas pipe network, part of residue liquid in biogas residue dehydration is discharged into the municipal gas pipe network after water treatment reaches the standard, benefits are maximized through the treatment process, and follow-up resource utilization of biogas residues is guaranteed; according to the project, resourceful treatment is fully realized; near-zero emission, the raw materials are cyanobacteria (blue-green algae and blue bacteria), kitchen waste, kitchen waste produced by urban catering enterprises and canteens and sludge produced by domestic sewage treatment plants. The invention not only can realize stabilization, harmlessness and reduction of cyanobacteria (blue-green algae and cyanobacteria), urban green plant wastes, sewage plant sludge and kitchen wastes, but also can convert wastes into valuable things to produce available biogas and fertilizers.

The invention discloses a genetically engineered bacterium for producing trehalose as well as a construction method and application of the genetically engineered bacterium, and belongs to the field ofgenetic engineering and fermentation engineering. The invention provides the genetically engineered bacterium for producing the trehalose as well as the construction method and application of the genetically engineered bacterium in order to solve the problems that an organic carbon source needs to be artificially provided as a raw material for producing trehalose at present and the production cost is increased. The genetically engineered bacterium is obtained by over-expressing maltooligosyl-trehalose synthase (MTSase), maltooligosyl-trehalose hydrolase (MTHase) and trehalose transport protein TRET1 by using cyanobacteria. The synthesis of trehalose by directly immobilizing carbon dioxide by solar energy in photoautotrophic microorganism cyanobacteria is realized. The trehalose synthesisenergy is from solar energy, and the carbon source is from carbon dioxide. Therefore, compared with the traditional production mode, the trehalose synthesized by the method is not limited by insufficient raw materials and energy, and meanwhile, the carbon emission is not increased, so that the trehalose is a true zero-emission trehalose product.