81 results about "Cyanobacterin" patented technology

The invention relates to the genetic modification of Cyanobacteria for the production of ethanol, and more particularly, to the genetic modification of Cyanobacteria by incorporating the genetic information encoding for pyruvate decarboxylase (pdc) and alcohol dehydrogenase (adh).

An IBTL system having a low GHG footprint for converting biomass to liquid fuels in which a biomass feed is converted to liquids by direct liquefaction and the liquids are upgraded to produce premium fuels. Biomass residues from the direct liquefaction, and optionally additional biomass is pyrolyzed to produce structured biochar, hydrogen for the liquefaction and upgrading, and CO2 for conversion to algae, including blue green algae (cyanobacteria) in a photobioreactor (PBR). Produced algae and diazotrophic microorganisms are used to produce a biofertilizer that also contains structured biochar. The structured biochar acts as a nucleation agent for the algae in the PBR, as a absorption agent to absorb inorganics from the biomass feed to direct liquefaction or from the liquids produced thereby, and as a water retention agent in the biofertilizer. The ratio of cyanobacteria to diazotrophic microorganisms in the biofertilizer can be selected to optimize the so as to achieve desired total chemically active carbon and nitrogen contents in the soil for a given crop.

The invention provides the disclosure of a novel plant / algae / cyanobacteria photosynthesis, biomass production, and productivity pathway involving low carbon dioxide inducible (LCI) proteins. According to the invention, the activity of one or more LCI proteins may be modulated to increase the same under conditions where such proteins are typically repressed. According to the invention, modulation of LCI protein activity was able to increase biomass production by as much as 80% under elevated CO2 conditions. The invention includes methods, and genetically modified plants / algae / cyanobacteria, cells, plant parts and tissues.

The present invention provides novel photobioreactors, modules thereof, and methods for use in culturing and harvesting algae and cyanobacteria.

The invention relates to acyl-CoA-independent methods of producing a wax ester in recombinant host cells engineered to express an acyl-ACP wax ester synthase, and an alcohol-forming acyl-ACP reductase. The methods of the invention may take place in photosynthetic microorganisms, and particularly in cyanobacteria. Isolated nucleotide molecules and vectors expressing an acyl-ACP wax ester synthase and / or an alcohol-forming acyl-ACP reductase, recombinant host cells expressing an acyl-ACP wax ester synthase and optionally an alcohol-forming acyl-ACP reductase, and systems for producing a wax ester via an acyl-CoA-independent pathway, are also provided.

Disclosed is a modified photoautotrophic bacterium comprising genes of interest that are modified in terms of their expression and / or coding region sequence, wherein modification of the genes of interest increases production of a desired product in the bacterium relative to the amount of the desired product production in a photoautotrophic bacterium that is not modified with respect to the genes of interest.

A photobioreactor system and the methods of using the system for cultivating photosynthetic organisms, including algae, cyanobacteria, plants, and plant cells. A single photobioreactor unit consists of transparent materials (such as glass, fiberglass, or plastics) and non-transparent materials (such as stainless steel or other metals). Two or more photobioreactor units can be connected with each other in a cascade to form a photobioreactor system, which can be used for cultivation of the photosynthetic organisms.

The invention relates to the fields of gene engineering and biosynthesis, and concretely relates to a construction body for efficiently synthesizing ethylene, and a construction method and an application thereof. The construction body comprises an active promoter and an ethylene synthetase gene (efe) controlled by the promoter. Carbon dioxide can be immobilized by above engineering bacterium through using solar energy in order to synthesize ethylene. An ethylene synthesis approach is constructed in cyanobacterial cells in order to realize immobilization of carbon dioxide in photosynthetic microbal cyanobacterial cells through using solar energy and synthesis of ethylene, wherein energy for ethylene synthesis is from the solar energy, and a carbon source is carbon dioxide. The method can be used to prepare bioethylene without restriction of petroleum shortage, and the bioethylene does not increase carbon emission.

The invention relates to the field of synthesis of high additional value chemicals by microorganisms, in particular to a cyanobacteria strain capable of being used for glycosylglycerol production andan application thereof. The strain is cyanobacterium aponinum 1B1 which is preserved in CGMCC on the 2nd, March, 2017, the preservation number of CGMCC No. 13785. The alga strain screened is separatedfrom a natural ecological environment of a salt lake in Yuncheng, Shanxi. Compared with a mode cyanobacteria synechocystis PCC6803, the strain has higher GG synthetic ability and adaptive capacity toenvironment. A compatible matter synthesized by the alga strain under salt stress is glycosylglycerol, so that the product is separated favorably. Therefore, the strain is quite suitable for being used as a production strain for glycosylglycerol and is applied to industrial production.

The invention discloses a new function of cyanobacteria naturally existing in nature, and aims to provide a photosensitizer with a simple preparation process, overcome a tumor hypoxia microenvironmentthrough oxygen self-production by utilizing the characteristic that cyanobacteria generates oxygen through photosynthesis after being illuminated, and target tumors to play an effective photodynamicrole. The in-vitro experiments prove that the cyanobacteria can effectively inhibit the growth of 4T1 cells after being irradiated by laser with a wavelength of 660nm. The photosensitizer is simple inprocess step and low in cost, only a large amount of cyanobacteria needs to be simply multiplied and cultured, and oxygen can be supplied to the photosensitizer by providing specific illumination, sothat tumor hypoxia is overcome, the photodynamic therapy effect is achieved, and the photosensitizer capable of generating oxygen by itself and having industrial practical value is developed.

This disclosure provides a method to improve lysine and methionine content of algae and cyanobacteria through genetic modification in combination with modified expression of high lysine and methionine proteins as sinks for the amino acids. The method of this disclosure is specifically useful in animal feed production.

Described herein are cyanobacteria encapsulated in hydrogels. Also described herein are consortia that include encapsulated cyanobacteria and heterotrophic microbes. The encapsulated cyanobacteria can be autotrophic and can provide nutrients to the heterotrophic microbes, which can utilize the nutrients to grow and produce useful products.

The invention discloses a new function of cyanobacteria. Under irradiation of specific wave length lasers, and through photosynthesis, oxygen is generated, the condition of oxygen deficiency caused bytumors can be improved, and the problem of reverse tolerance of chemicals, such as adriamycin can be effectively solved. The cyanobacteria is subjected to photosynthesis in light to generate oxygen,and under the aerobic condition, hypoxia factors are reduced for tumor cells, so that multidrug resistance genes can be restrained, expression of P-glycoprotein is restrained to restrain exocytosis ofcancer cells on adriamycin, the reverse tolerance of the cancer cells on the reverse tolerance is overcome, and the antitumor treatment effect can be increased.

The invention relates to a construction body, comprises a carrier of the construction body, comprises the construction body or cyanobacteria converted by the carrier, and relates to a method for improving fatty alcohol yield in cyanobacteria, wherein the cyanobacteria is modified to be capable of producing fatty alcohol. The invention also relates to a new method for producing fatty alcohol in cyanobacteria.

The invention provides a cyanobacterium of a living drug. Oxygen is generated by means of photosynthesis under laser radiation to improve tumor hypoxia, so that the tumor microenvironment is changed,an oxygen-increasing tumor therapeutic effect is achieved, and novel selection is provided for cancer treatment. An in vitro experiment verifies that the cyanobacterium can generate oxygen infinitelyunder a condition of 660 nm laser radiation. An in vivo experiment verifies that the tumor hypoxia condition is improved obviously by tail vein injection of the cyanobacterium into a mouse body and radiation on mouse tumors by 660 nm laser.

The present invention relates to compositions and methods for stable transformation of green microalgae and for production of transgenic green microalgae and / or cyanobacteria that produce extremophile enzymes as co-products during the growth of the green microalgae and / or cyanobacteria for lipid biofuel production. Thus, the present invention provides nucleic acid constructs and methods of transformation useful in the production of stably transformed green microalgae and / or cyanobacteria expressing extremophile enzymes in combination with lipid production for biofuel.

The invention discloses a preparation method of a phytochrome-derived yellow fluorescent label. According to the preparation method, a fusion protein comprises the 3th GAG domain of cyanobacteria phytochrome protein slr1393 and streptavidin. Stable expression of the fusion protein sequence can be realized in microorganisms; defects such as acquisition difficulty of high purity natural phycobiliprotein and cyanobacteria phytochrome, high preparation cost, applications of chemical modifying agents, and unstable natural phycobiliprotein polymeric species are avoided. In the expression method, nophycobiliprotein lyase is needed, the number of converted genes is reduced, bacterial strain stability and screening efficiency are increased; and optimization of fermentation culture medium and fermentation conditions is capable of increasing fusion protein yield greatly.

The invention relates to a microbial agent filler for environmentally-friendly ecological toilets. A microbial agent comprises penicillium, streptomyces, saccharomycetes, lactic acid bacteria, cyanobacteria, bacillus, pseudomonas, trichoderma, rhodococcus and sulfuration-reduction bacteria, and after the microbial agent is cultured, the microbial agent is mixed with a sterilized filler base material containing sawdust, rice husks, peanut shells and corn starch to obtain the filler. The microbial agent filler is used for harmless treatment of feces in microbial environmentally-friendly ecological toilets, and a degraded product is used as an organic fertilizer.

The present invention relates to a stabilized water treatment composition, containing (i) an effective amount of a hydrogen peroxide source, (ii) a first stabilizing agent, and (iii) a second stabilizing agent; which is essentially free of any peracid-based compounds, derivatives or salts thereof. A method for the using the stabilized water treatment composition to kill or to inhibit the growth of microorganisms such as gram positive or gram negative bacteria, algae, cyanobacteria, viruses, fungi, mildew, mold or combinations thereof, for various water sources such as streams, ponds, lakes, recirculating water systems, surface water, and any other suitable or desirable water sources is also described.

The invention discloses a streptavidin and phytochrome fused yellowish-green fluorescent indicator preparation method. A fusion protein comprises a second GAF structural domain of cyanobacterium phytochrome protein all1280 and streptavidin. A sequence of the fusion protein can be easily and stably expressed in microorganisms, and defects of difficulty in acquisition of high-purity natural phycobiliprotein and cyanobacterium phytochrome, high preparation cost, chemical modifier consumption, polymerization morphological instability of natural phycobiliprotein and the like are avoided. The expression method is free of phycobiliprotein lyase participation, transformational gene amount is decreased, and strain stability and screening efficiency are improved. In addition, by optimization of fermentation media and fermentation conditions, fusion protein yield is greatly increased.

The invention discloses a green fluorescent cyanobacterium phytochrome fluorescence indicator preparation method. A fusion protein comprises a second GAF structural domain of cyanobacterium phytochrome protein all3691 and streptavidin. A sequence of the fusion protein can be easily and stably expressed in microorganisms, and defects of difficulty in acquisition of high-purity natural phycobiliprotein and cyanobacterium phytochrome, high preparation cost, chemical modifier consumption, polymerization morphological instability of natural phycobiliprotein and the like are avoided. The expressionmethod is free of phycobiliprotein lyase participation, transformational gene amount is decreased, and strain stability and screening efficiency are improved. In addition, by optimization of fermentation media and fermentation conditions, fusion protein yield is greatly increased.

The invention discloses a saline-alkali soil heavy metal ion remover and a preparation method thereof. The saline-alkali soil heavy metal ion remover comprises the following raw material components byweight: 30-45 parts of mineral source humus ultrafine powder, 5-10 parts of a phanerochaete chrysosporium extracellular polymeric substance, 4-8 parts of cyanobacteria liquid, 15-20 parts of lignin grafted polyacrylonitrile, 5-10 parts of citric acid, 20-25 parts of straw powder, and 15-20 parts of desulfurized gypsum. The mineral source humus ultrafine powder in the soil desalting agent can improve the air permeability and water permeability of soil and strongly adsorb various heavy metal ions, also has strong water retention and storage capacity, and improves the landform of saline-alkali soil, so that the saline-alkali soil can adapt to more and more plant growth, and the selectivity of agricultural planting is improved.

The invention discloses a microbial fertilizer for promoting decomposition of soil organic matters. The microbial fertilizer comprises diatomite, bamboo powder, lentinan, yeast extract, vitamin C powder, lignin grafted polyacrylonitrile, microbial agent, cyanobacteria liquid and trace elements, wherein the microbial agent comprises bacillus thuringiensis, bacillus subtilis, bacillus megaterium, bacillus mucilaginosus, bacillus laterosporus, streptomyces jingyangensis and aspergillus niger. Tests prove that the microbial fertilizer in the embodiment of the invention can effectively increase theyield of bitter gourds planted in saline-alkali soil; and moreover, the microbial fertilizer can effectively reduce the pH of soil and the salt content and organic matter content in the soil. The preparation method of the microbial fertilizer is simple, related microbial strains are also common strains on the market, the use method is simple, the microbial fertilizer does not conflict with conventional fertilization, the yield increasing effect and the soil rational property improving effect are obvious, and the microbial fertilizer is suggested to be popularized and used in saline-alkali soil areas.

The present invention relates to methods of metabolic engineering cells to increase their ability to compete with contaminating microorganisms without the need for antibiotics. More particularly, the invention provides methods to engineer cyanobacteria to utilize melamine as nitrogen source, phosphite as phosphorous source, optionally also utilizing NADP<+> over NAD<+>, and also provides genetically engineered cyanobacteria made using such methods. In a particular embodiment, the genetically engineered cells are cyanobacterium transformed by at least one polynucleotide molecule comprising heterologous melamine utilization pathway genes, atzD, trzE, DUR1,2, trzC, guaD and triA operably linked to at least one promoter and / or comprising a further phosphite dehydrogenase (ptxD ) gene.

A method for obtaining biofertilizer concentrates and biostimulants for agricultural use from biomass of microalgae, including cyanobacteria, wherein, firstly, the cellular content of the microalgae, including cyanobacteria, is released by rupturing the cells thereof; next, the protein fraction and the cell wall of the microalgae, including cyanobacteria, obtained from said release of the cellular content, are hydrolyzed with proteolytic enzymes until producing free amino acids; next, nitrogen (N), phosphorus (P) and / or potassium (K) excipients are added to the previously obtained product of enzymatic hydrolysis.

The invention discloses a method for treating cyanobacteria green plant sewage plant and kitchen waste through cooperation of photoelectricity and heat, and belongs to the technical field of environmental protection and renewable energy source treatment. After high-temperature pyrohydrolysis / high-temperature disinfection and sterilization, municipal sewage plant organic sludge, municipal organic kitchen waste, municipal other organic matter waste, blue algae and other waste are subjected to high-temperature anaerobic digestion, biogas residue dehydration, high-pressure squeezing and solar drying, finally, resource utilization is carried out, biogas is generated during high-temperature anaerobic digestion treatment, and the biogas is conveyed into a biogas storage cabinet and is collected.Resourceful treatment and the comprehensive treatment process of direct squeezing of a biogas residue dehydrator dehydration high-pressure filter press and solar drying are fully realized, stabilization, harmlessness and reduction of blue-green algae (blue-green algae and blue bacteria), urban green plant waste, sewage plant sludge and kitchen waste can be achieved, meanwhile, waste is turned intowealth, and available biogas and fertilizer are produced.

Microorganisms with increased photosynthetic capacity are described. Increased photosynthetic capacity is achieved by down-regulating activity of the RpaB pathway. The microorganisms include Cyanobacteria, including genetically-modified Cyanobacteria.

An ICBTL system and method having a low GHG footprint for converting coal or coal and biomass to liquid fuels and a biofertilizer in which a carbon-based feed is converted to liquids by direct liquefaction and optionally by indirect liquefaction and the liquids are upgraded to produce premium fuels. CO2 produced by the process is used to a produce cyanobacteria containing algal biomass and other photosynthetic microorganisms in a photobioreactor. Optionally, lipids extracted from the some of the algal biomass is hydroprocessed to produce fuel components and biomass residues and the carbon-based feed our gasified to produce hydrogen and syngas for the direct and indirect liquefaction processes. Some or all of the algal biomass and photosynthetic microorganisms are used to produce a natural biofertilizer. CO2 may also be produced by a steam methane reformer for supplying CO2 to produce the algal biomass and photosynthetic microorganisms.

Methods of inoculum scale-up of cyanobacteria to commercial-scale closed photobioreactors for the production of a target molecule such as ethanol.