59 results about "Cyanobacterin" patented technology

The invention belongs to the field of biological organic fertilizers, and discloses a special organic selenium-rich fertilizer for grapes as well as a preparation method and application thereof. The fertilizer is prepared from the following raw materials in percentage by mass: 0.05-0.2% of a selenium complex, 2-15% of a stabilizer, 15-25% of a microbial fermentation product, 0.5-2.0% of a natural plant extract and the balance of auxiliary materials, totaling 100%. The microbial fermentation product is microbial fermentation liquid obtained by fermenting bacillus tequilensis and cyanobacteria; the auxiliary material is obtained by mixing sodium alginate and maltodextrin; the natural plant extract is obtained by mixing azadirachtin, sophocarpidine, tea tree essential oil and cnidium lactone. Through the synergistic effect of the microbial fermentation product, the natural plant extract and the auxiliary materials, the industrial problem that in the prior art, a selenium fertilizer can only improve the selenium content of grapes, but cannot effectively improve the disease resistance and insect pest resistance of the grapes and slow down selenium element loss is solved, and the double improvement of the grape quality and the selenium content is achieved.

Disclosed is a system for cultivating and harvesting a cyanobacterial biomass. The system comprises at least one vessel that, when in operation, grows the cyanobacterial biomass in a nutrient growth media under regulated growth conditions and a base unit configured to receive the at least one vessel. The system also comprises at least one light source and a cultivation air pump that, when in operation, supplies light, heat and air to the at least one vessel. Notably, the cultivation air pump supplied air via a one-way air valve in the at least one vessel. The system also comprises at least one photodiode that, when in operation, measure a cyanobacterial biomass concentration in the at least one vessel. Disclosed also is a method for cultivating and harvesting a cyanobacterial biomass.

By engineering genetic traits associated with photosynthesis in plants, transgenic plants with increased crop yield can be generated, which will ultimately lead to increased food production in the field. To obtain beneficial genetic traits for enhanced photosynthesis, accelerated evolution is applied under salt stress in a cyanobacteria species, Synechococcus elongatus PCC 7942, by conditionally suppressing its mismatch repair system. Through high-throughput screening for biomass accumulation and sucrose production, it is discovered that specific amino acid changes in the pre-Dl protein of Photosystem II (PSII), encoded by the psbA genes, can improve efficiency of photosynthesis under salt stress, as well as pathogen resistance. Next, the same amino acid changes were made in Arabadopsis and proved that photosynthetic efficiency (measured via growth rate) is also increased in this model vascular plant.

The present disclosure provides a new antioxidant using cyanobacteria. An antioxidant according to the present disclosure contains a secretion product of cyanobacteria.

The invention relates to application of boron nitride in microalgae culture and a method for culturing microalgae, which comprises the step of adding boron nitride into a culture system. By constructing a hexagonal boron nitride-cyanobacteria co-culture system and combining the carbon dioxide adsorption performance of a nano material and the biological directional synthesis capability of cyanobacteria, more efficient carbon dioxide capture and conversion are realized, and meanwhile, the growth speed and biomass of cyanobacteria and the yield of a target product are also improved. The innovative strategy not only can enhance the photosynthetic carbon sequestration efficiency of cyanobacteria, but also provides a new technical route for promoting green and low-carbon development, and gives consideration to both high economic benefit and low-carbon environmental protection.

The invention discloses a function unannotated protein CpsA and application thereof, and belongs to the technical field of biochemistry. The CpsA protein provided by the invention is a thylakoid membrane protein which is coded by synpcc79421022 in synechococcus slender PCC 7942, and the function of the thylakoid membrane protein is not annotated. The protein can be specifically combined with an early PSII assembly factor Psb28, formation and stability of a PSII final oxygen evolution complex are promoted, and the assembly efficiency of a light system II and formation of a functional complex are remarkably improved. In addition, the CpsA protein is highly conserved in cyanobacteria, microalgae and land plants, and has wide application potential.

Provided herein are compositions, methods and systems for precipitating metallic carbonate using cyanobacteria. The precipitated metallic carbonate can be of high purity and / or has a biogenic signature associated with the one or more cyanobacteria.

The invention relates to a kaiA gene mutant capable of improving the growth speed of cyanobacteria, the 266th-273rd amino acids are shown as SEQ ID NO: 5, and the 274th amino acids and the amino acids behind the 274th amino acids are deleted; the invention also relates to application of the kaiA gene mutant in improving the growth speed of cyanobacteria and a constructed fast-growing engineering strain. According to the invention, the kaiA gene of synechococcus PCC 7942 is mutated, so that the 266th-273rd amino acids of the coded protein are changed, and the 274th and subsequent amino acids are deleted, so that the obtained mutant strain has a faster growth speed, and the increase of the growth speed has a synergistic effect with AtpA C252Y mutation. Therefore, the growth speed of the synechococcus can be greatly improved by performing corresponding genetic modification on the synechococcus, and the yield of the microalgae and the target compound can be improved by using the mutant strain as the chassis algae for metabolic modification and production of the target compound.

A method for cultivating cyanobacteria includes: providing the cyanobacteria; subjecting the cyanobacteria to physical mutagenesis, so as to obtain primary mutant cyanobacteria; subjecting the primary mutant cyanobacteria to chemical mutagenesis, so as to obtain secondary mutant cyanobacteria; and subjecting the secondary mutant cyanobacteria to a temperature resistance test and an environment resistance test, so as to obtain target cyanobacteria. A fatality rate of the physical mutagenesis ranges between 50% and 80%, and a fatality rate of the chemical mutagenesis ranges between 40% and 55%.

The invention belongs to the technical field of preparation of concrete through microorganisms, and particularly relates to a method for preparing a concrete super-hydrophobic surface through microorganisms. The invention provides a method for preparing a concrete super-hydrophobic surface by microorganisms, which comprises the following steps: smearing a cyanobacteria culture solution on the surface of concrete, and mixing to obtain cyanobacteria concrete; and the cyanobacteria concrete surface is subjected to smoothing treatment and mineralization treatment, and the concrete super-hydrophobic surface is obtained. The cyanobacteria disclosed by the invention are wide in distribution, easy to culture and extremely low in cost. The concrete is in contact with cyanobacteria, the cyanobacteria secretes a large amount of acid compounds, cement in the concrete is decomposed, the content of calcification compounds in the concrete is increased, a mineralization layer is conveniently formed on the surface of the concrete, and the hydrophobic effect is guaranteed. Meanwhile, the concrete surface is smoothed, so that the concrete surface is smooth, and the hydrophobic effect of the concrete is improved. And through mineralization treatment, drying of the surface of the concrete is accelerated, and a super-hydrophobic structure is formed.

This invention is an artist paint comprising a colorant and a water-based paint vehicle. The colorant comprises whole microalgal or cyanobacterial cells, which are grown under controlled light conditions comprising changes in light intensity or color for specified periods of time to produce the desired colorant. The water-based paint vehicle comprises a humectant, photostabilizers and preservatives, a buffer, and rheology modifiers. The paint is made by growing and harvesting the cells as the colorant followed by mixing with the water-based paint vehicle to create a paint suspension. The resulting suspension is allowed to dehydrate to form a moist cake. The cake is mixed with the water-based paint vehicle using a paint brush to create a second paint suspension that is then painted onto a surface. The resulting paint can be controlled to provide a uniform or a gritty texture.

The invention provides a microbial composition for realizing nitrogen reduction and synergy of a flooded rice field as well as a preparation method and application of the microbial composition, and belongs to the technical field of rice field nitrogen reduction. The microbial composition disclosed by the invention comprises cyanobacteria microspheres and pseudomonas fluorescens microspheres, wherein the mass ratio of the cyanobacteria microspheres to the pseudomonas fluorescens microspheres is (4.8-5.2): (2.8-3.2); the cyanobacteria microspheres and the pseudomonas fluorescens microspheres are independently packaged. The cyanobacteria microspheres are spread on the water surface of a rice field 7-10 days before rice transplanting; in the early tillering stage of the rice, the pseudomonas fluorescens microspheres are applied to a 5-10cm soil layer at the rhizosphere of the rice, so that the nitrogen loss amount of the flooded rice field is reduced, the nitrogen fertilizer utilization rate and the rice yield of the rice are improved, and a technical support is provided for ensuring the stable yield and yield increase of the rice under the condition that the nitrogen fertilizer application amount of the flooded rice field is reduced.

The present invention relates to a method for constructing an engineered cyanobacteria that tolerates high temperature and high light, including the step of increasing the expression amount of shikimate kinase in the cyanobacteria; and also relates to an engineered cyanobacteria that grows rapidly under high temperature and high light conditions constructed on the basis of the engineered cyanobacteria that tolerates high temperature and high light and a construction method thereof. The engineered cyanobacteria constructed by the present invention can grow rapidly and stably under high temperature and high light conditions that the wild type cannot tolerate, and solves the problem of decreased or unstable target product yield caused by high temperature and high light stress in outdoor large-scale cultivation. In addition, whether under normal temperature and normal light conditions or under high temperature and high light stress conditions, the engineered cyanobacteria of the present invention has a significantly higher growth rate than its wild type, can accumulate more biomass, synthesize more glycogen, and can accumulate more protein under high temperature and high light conditions, so the chassis bacteria as a "cell factory" have the potential to produce target products more efficiently.

The invention relates to an NAD (P) H dehydrogenase subunit gene ndhE mutant capable of increasing the growth speed of cyanobacteria. A section of amino acid with the sequence shown as SEQ ID NO: 5 is added to the tail of a coded protein; the invention also relates to application of the ndhE mutant in improving the growth speed of cyanobacteria and a constructed fast-growing engineering strain. According to the invention, ndhE of synechococcus PCC 7942 is mutated, so that a section of amino acid with a sequence as shown in SEQ ID NO: 5 is added at the tail of a protein coded by the synechococcus PCC 7942, the obtained mutant strain has a faster growth speed in highlight, and the increase of the growth speed has a synergistic effect with AtpA C252Y mutation. Therefore, the growth speed of the synechococcus can be greatly improved by performing corresponding genetic modification on the synechococcus, and the yield of the microalgae and the target compound can be improved by using the mutant strain as the chassis algae for metabolic modification and production of the target compound.

The invention discloses a culture nutrient solution suitable for directionally improving the maximum water-holding capacity of coral island calcareous sand cyanobacteria crust. The culture nutrient solution contains 25g / L of glucose, 0.025 g / L of sodium dihydrogen phosphate, 0.1 g / L of potassium chloride, 0.05 g / L of magnesium chloride, 0.1 g / L of sodium nitrate, 0.005 g / L of ferrous chloride and the balance of water. Based on the cyanobacteria crust collected in situ, the hydrological characteristics of the cyanobacteria crust from the coral island calcareous sand are directionally improved by utilizing the artificially prepared culture nutrient solution, so that the development of the cyanobacteria crust can be promoted, the maximum water holding capacity of the crust can be improved, the use amount of an inoculation material is saved, the influence on the environment is reduced, and the economic benefit is increased. Therefore, the accumulation capacity of fresh water in the coral island is improved, a good material foundation is laid for change of an ecological environment system of the coral island, and the method has wide application value.

A method and system produce fertilizer products by growing cyanobacteria in a photobioreactor, the cyanobacteria performing nitrogen fixation using Nitrogen from the ambient air brought into the photobioreactor. The grown cyanobacteria is harvested from the photobioreactor as biomass, which is subject to anaerobic digestion in an anaerobic digestion reactor, to produce Ammonium, which is optionally subjected to nitrification, in a nitrification bioreactor, to produce the resultant fertilizer product.

The invention belongs to the technical field of agricultural biology and the technical field of compound fertilizers, and discloses an active cyanobacteria dry land fertilizer and a preparation method and application thereof.The method comprises the following steps that cyanobacteria is cultured and harvested to obtain cyanobacteria concentrated pulp, then the cyanobacteria concentrated pulp is rinsed with a drying protective agent, and the active cyanobacteria dry land fertilizer is obtained; preferably, symbiotic rhizobium is added into the cyanobacteria concentrated slurry, and the cyanobacteria concentrated slurry is mixed with a matrix material to obtain a cyanobacteria embedding material; then drying and crushing the cyanobacteria concentrated slurry or the cyanobacteria embedding material to obtain cyanobacteria dry powder; and adding the cyanobacteria dry powder into a plant fertilizer or an edible fungus fertilizer or water or a soil improvement product to obtain the active cyanobacteria dry land fertilizer. Based on adsorption and embedding of plant growth promoting substances contained in cyanobacteria by the drying protective agent, the active cyanobacteria dry land fertilizer has the advantages of being resistant to storage and transportation, capable of remarkably promoting plant growth, suitable for various field environments, capable of improving soil and increasing fertility and the like.

A method for producing phosphatidylethanolamine with use of a carbon source includes: cultivating modified cyanobacteria, in which the modified cyanobacteria are cultivated in a liquid medium; undergoing a photo-fermentation process, in which the carbon source is provided to the modified cyanobacteria, so that the modified cyanobacteria convert the carbon source into serine; undergoing a filtration process, in which the liquid medium is filtered for separating the modified cyanobacteria, so as to obtain the filtered liquid medium; synthesizing the phosphatidylethanolamine, in which the filtered liquid medium and the serine are mixed with a lecithin and a phospholipase in a photoreactor, so as to obtain a phosphatidylethanolamine mixture; and undergoing an oil-water separation process, in which the phosphatidylethanolamine mixture is placed in an oil water separator and left to rest for a predetermined period of time, and the phosphatidylethanolamine is obtained from an upper layer of the phosphatidylethanolamine mixture.

The invention relates to a method for harvesting liquid culture of cyanobacteria. The method comprises the step of inducing cultured cyanobacteria cells to increase the length of the cyanobacteria cells before harvesting. According to the method, a light-sensitive gene regulation and control system and a gene which is regulated and controlled by the light-sensitive gene regulation and control system and affects the cellular morphology are transferred into target cyanobacteria cells, and expression of genes related to the cellular morphology is induced through irradiation of green light, so that the cell length is successfully increased; therefore, the cultured cyanobacteria can be harvested more efficiently by using a filter membrane filtration mode.

The growth of algae can be controlled in water by exposing water containing algae to agitation, for example shear stress and an electrical field. The water may further be detoxified of the microcystins produced by algae by treating the water with a microcystin-degrading bacterium, such as Sphingomonas sp. together with an electric field, with the application of the electric field causing a major acceleration of the toxin's degradation.

The application discloses a kind of cultivation nutrient solution suitable for directional improvement of maximum water-holding capacity of calcareous sand cyanobacterial crust of coral island.The cultivation nutrient solution contains: glucose 25g / L, sodium dihydrogen phosphate 0.025g / L, potassium chloride 0.1g / L, magnesium chloride 0.05g / L, sodium nitrate 0.1g / L, ferrous chloride 0.005g / L, and the balance is water.The application is based on in-situ collection of cyanobacterial crust, and uses artificial preparation of cultivation nutrient solution to directionally improve hydrological characteristics of cyanobacterial crust from calcareous sand of coral island, which can promote the development of cyanobacterial crust, improve the maximum water-holding capacity of crust, save the use amount of inoculation material, reduce the influence on environment, thereby improve the storage capacity of freshwater of coral island, lay a good material foundation for the change of coral island ecological environment system, and therefore have wide application value.

The present invention relates to an anti-inflammatory extract of soil microorganisms or a fraction thereof, wherein the microorganisms comprise one or more selected from Chlorophyta, Xanthophyceae or Cyanobacteria. The invention further relates to compositions comprising two or more of Lyso-DGTS, p-Coumaric acid (ester-linked), oleamide, theophylline, or DGTS. The invention further relates to pharmaceutical compositions comprising the anti-inflammatory extract of soil microorganisms or a fraction thereof or a composition comprising two or more of Lyso-DGTS, p-Coumaric acid (ester-linked), oleamide, theophylline, or DGTS as an active ingredient. The invention further relates to an anti-inflammatory extract of soil microorganisms or a fraction thereof or a pharmaceutical composition comprising an anti-inflammatory extract of soil microorganisms or a fraction thereof or a composition comprising two or more of Lyso-DGTS, p-Coumaric acid (ester-linked), oleamide, theophylline, or DGTS as an active ingredient for use as a medicament, wherein the microorganisms comprise one or more selected from Chlorophyta, Xanthophyceae or Cyanobacteria. The invention further relates to compositions comprising an anti-inflammatory extract of soil microorganisms or a fraction thereof, wherein the microorganisms comprise one or more selected from Chlorophyta, Xanthophyceae or Cyanobacteria.

The present disclosure provides a method for determining deprivation of an outer membrane of a cyanobacterium with which whether an outer membrane is deprived from a cell wall can be readily determined. The method for determining deprivation of an outer membrane of a cyanobacterium according to the present disclosure includes a measurement step (S01) in which a concentration of at least one amino acid selected from the group consisting of isoleucine, leucine, tyrosine, and phenylalanine in a culture supernatant of a cyanobacterium is measured and a determination step (S02) in which whether an outer membrane of the cyanobacterium is deprived from a cell wall of the cyanobacterium is determined on the basis of the concentration of the at least one amino acid, the concentration being measured in the measurement step.

The invention relates to methods and systems for collecting environmental pollutants from open water bodies, in particular the concentration of a biomass of a cyanobacteria and water mixture. The filtration and concentration of the biomass of the cyanobacteria and water mixture is carried out using a plankton net (3). The proposed concentration method and device allows to obtain dense biomass in a single continuous process. The biomass of the cyanobacteria and water mixture distributed on the plankton net (3) is divided into shorter parts of the biomass by partitions (8) of a biomass pusher which is pushed back and forth within the plankton net (3) until the biomass loses water through the plankton net (3) and reaches the desired concentration. The biomass pusher then pushes off the biomass from the plankton net (3) in repeated movement cycles until all biomass parts between the partitions (8) have successively reached the outlet end of the plankton net (3) and are pushed away from it.

A modified cyanobacterium in which an organic channel protein which improves protein permeability of an outer wall is expressed.

The present disclosure provides novel antioxidants using cyanobacteria. The antioxidants of the present disclosure comprise secretions of cyanobacteria.

A biological desalination process to remove salt from seawater using naturally grown algae or cyanobacteria in an attached growth photobioreactor setup. The photobioreactor (PBR) structure can be vertical or horizontal in orientation. Any shape of PBR can provide more light contact to the algae or cyanobacteria for enhanced growth on support media. The reactor can be designed in a way that can get maximum exposure to sunlight. The influent seawater is mixed with nutrients that are essential for the growth of algae or cyanobacteria. The main objective is to remove the salt from the brackish, saline, hypersaline or seawater. The influent seawater can be treated using the attached growth of algae or cyanobacteria, and the effluent from the PBR can be further treated based on desired water use.

The invention relates to application of high-temperature-resistant and high-light cyanobacteria in preparation of a biological photovoltaic device, the high-temperature-resistant and high-light cyanobacteria is synechococcus PCC 7942 obtained by introducing C252Y mutation on an ATP synthase alpha subunit, and the reference sequence of the ATP synthase alpha subunit is shown as SEQ ID NO: 1. According to the invention, a high-tolerance mutant strain HL7942 is used as a photoelectric conversion core element, so that a biological membrane cathode is formed on a substrate, a biological photovoltaic device can stably carry out light energy conversion output under the condition of high temperature and high light (2400 [mu] mol photons m <-2 > s <-1 >, 35 DEG C), and the light current output level is increased by 2.54 times compared with that of an early-stage device. Chitosan is used for packaging a biological membrane electrode, so that a biological membrane is stably attached to a substrate while the photosynthetic activity is maintained, the photocurrent output density of the device is improved, and the stable output period of the device is prolonged.

The present disclosure provides a method for determining exfoliation of an outer membrane of cyanobacteria capable of easily determining whether or not the outer membrane has been exfoliated from a cell wall. A method for determining exfoliation of an outer membrane of cyanobacteria according to the present invention comprises: a measurement step (S01) for measuring the concentration of at least one amino acid selected from the group consisting of isoleucine, leucine, tyrosine and phenylalanine in a culture supernatant of cyanobacteria; and a determination step (S02) for determining whether or not the outer membrane of the cyanobacteria has been peeled off from the cell wall on the basis of the concentration of the at least one type of amino acid measured in the measurement step.

An indoor hydroponic device uses cyanobacteria as fertilizer, examining the media composition and nutrient delivery to this hydroponic unit. Due to the use of this fertilizer and the hydroponic setup, the device requires minimal space and maintenance. The device focuses on a) optimizing cyanobacteria and plant growth, b) increasing the efficacy of cyanobacteria as fertilizer, and c) evaluating and enhancing the user's experience with the hydroponic unit.