25 results about "Cyanobacterin" patented technology

By engineering genetic traits associated with photosynthesis in plants, transgenic plants with increased crop yield can be generated, which will ultimately lead to increased food production in the field. To obtain beneficial genetic traits for enhanced photosynthesis, accelerated evolution is applied under salt stress in a cyanobacteria species, Synechococcus elongatus PCC 7942, by conditionally suppressing its mismatch repair system. Through high-throughput screening for biomass accumulation and sucrose production, it is discovered that specific amino acid changes in the pre-Dl protein of Photosystem II (PSII), encoded by the psbA genes, can improve efficiency of photosynthesis under salt stress, as well as pathogen resistance. Next, the same amino acid changes were made in Arabadopsis and proved that photosynthetic efficiency (measured via growth rate) is also increased in this model vascular plant.

Provided herein are compositions, methods and systems for precipitating metallic carbonate using cyanobacteria. The precipitated metallic carbonate can be of high purity and / or has a biogenic signature associated with the one or more cyanobacteria.

The invention belongs to the technical field of preparation of concrete through microorganisms, and particularly relates to a method for preparing a concrete super-hydrophobic surface through microorganisms. The invention provides a method for preparing a concrete super-hydrophobic surface by microorganisms, which comprises the following steps: smearing a cyanobacteria culture solution on the surface of concrete, and mixing to obtain cyanobacteria concrete; and the cyanobacteria concrete surface is subjected to smoothing treatment and mineralization treatment, and the concrete super-hydrophobic surface is obtained. The cyanobacteria disclosed by the invention are wide in distribution, easy to culture and extremely low in cost. The concrete is in contact with cyanobacteria, the cyanobacteria secretes a large amount of acid compounds, cement in the concrete is decomposed, the content of calcification compounds in the concrete is increased, a mineralization layer is conveniently formed on the surface of the concrete, and the hydrophobic effect is guaranteed. Meanwhile, the concrete surface is smoothed, so that the concrete surface is smooth, and the hydrophobic effect of the concrete is improved. And through mineralization treatment, drying of the surface of the concrete is accelerated, and a super-hydrophobic structure is formed.

This invention is an artist paint comprising a colorant and a water-based paint vehicle. The colorant comprises whole microalgal or cyanobacterial cells, which are grown under controlled light conditions comprising changes in light intensity or color for specified periods of time to produce the desired colorant. The water-based paint vehicle comprises a humectant, photostabilizers and preservatives, a buffer, and rheology modifiers. The paint is made by growing and harvesting the cells as the colorant followed by mixing with the water-based paint vehicle to create a paint suspension. The resulting suspension is allowed to dehydrate to form a moist cake. The cake is mixed with the water-based paint vehicle using a paint brush to create a second paint suspension that is then painted onto a surface. The resulting paint can be controlled to provide a uniform or a gritty texture.

The invention provides a microbial composition for realizing nitrogen reduction and synergy of a flooded rice field as well as a preparation method and application of the microbial composition, and belongs to the technical field of rice field nitrogen reduction. The microbial composition disclosed by the invention comprises cyanobacteria microspheres and pseudomonas fluorescens microspheres, wherein the mass ratio of the cyanobacteria microspheres to the pseudomonas fluorescens microspheres is (4.8-5.2): (2.8-3.2); the cyanobacteria microspheres and the pseudomonas fluorescens microspheres are independently packaged. The cyanobacteria microspheres are spread on the water surface of a rice field 7-10 days before rice transplanting; in the early tillering stage of the rice, the pseudomonas fluorescens microspheres are applied to a 5-10cm soil layer at the rhizosphere of the rice, so that the nitrogen loss amount of the flooded rice field is reduced, the nitrogen fertilizer utilization rate and the rice yield of the rice are improved, and a technical support is provided for ensuring the stable yield and yield increase of the rice under the condition that the nitrogen fertilizer application amount of the flooded rice field is reduced.

The application discloses a kind of cultivation nutrient solution suitable for directional improvement of maximum water-holding capacity of calcareous sand cyanobacterial crust of coral island.The cultivation nutrient solution contains: glucose 25g / L, sodium dihydrogen phosphate 0.025g / L, potassium chloride 0.1g / L, magnesium chloride 0.05g / L, sodium nitrate 0.1g / L, ferrous chloride 0.005g / L, and the balance is water.The application is based on in-situ collection of cyanobacterial crust, and uses artificial preparation of cultivation nutrient solution to directionally improve hydrological characteristics of cyanobacterial crust from calcareous sand of coral island, which can promote the development of cyanobacterial crust, improve the maximum water-holding capacity of crust, save the use amount of inoculation material, reduce the influence on environment, thereby improve the storage capacity of freshwater of coral island, lay a good material foundation for the change of coral island ecological environment system, and therefore have wide application value.

A modified cyanobacterium in which an organic channel protein which improves protein permeability of an outer wall is expressed.

The present disclosure provides novel antioxidants using cyanobacteria. The antioxidants of the present disclosure comprise secretions of cyanobacteria.

An indoor hydroponic device uses cyanobacteria as fertilizer, examining the media composition and nutrient delivery to this hydroponic unit. Due to the use of this fertilizer and the hydroponic setup, the device requires minimal space and maintenance. The device focuses on a) optimizing cyanobacteria and plant growth, b) increasing the efficacy of cyanobacteria as fertilizer, and c) evaluating and enhancing the user's experience with the hydroponic unit.

The present invention relates to a photosensitive polar biomass extract extracted from phycocyanin-producing organisms, in particular unicellular red algae (URA) or cyanobacteria, and to the cosmetic use of the photosensitive polar biomass extract for preventing and / or eliminating skin defects, i.e. For improving the appearance of the skin.

This invention relates to the field of resource and environmental technology, specifically to a method and application of an algae-bacteria hybrid system for treating high ammonia nitrogen wastewater. By regulating nutrient and operational parameters, a functional community is constructed and maintained, dominated by microalgae and supplemented by bacteria and cyanobacteria, with their relative abundance within a preset optimized range. A clear interspecific interaction network forms within the system: microalgae such as *Nostoc* and *Chlorella* exhibit mutualistic symbiosis, while bacteria such as *Flavobacterium* show antagonistic relationships with these microalgae. Positive and negative feedback mechanisms maintain community balance and system robustness. The three components complement each other: microalgae primarily drive photocatalytic nitrogen and phosphorus removal, cyanobacteria provide nitrogen fixation and stability, and functional bacteria participate in organic matter degradation and nitrogen cycling, achieving synergistic metabolism through material exchange. Microbial ecological analysis confirms that a stable community structure and internal interaction mechanisms are the core of efficient and stable ammonia nitrogen reduction. This technology offers precise regulation, stable operation, and is environmentally friendly and economical, and can be widely applied to the treatment of various types of ammonia nitrogen-polluted water bodies.

Processes are disclosed for photosynthetic cyanobacterial production of selected proteins and biochemicals within an evolving alkaliphilic microbial consortium.

This invention provides compositions and methods for providing high level expression of proteins of interest in cyanobacteria.

There is provided an immunogenic composition comprising or consisting of a recombinant Cyanobacteria or lysate thereof wherein the recombinant Cyanobacteria comprises a nucleic acid encoding at least one VLP and at least one heterologous antigen, wherein the expressed VLP comprises the at least one heterologous antigen.

The invention discloses a method for observing local death of cyanobacteria cells by using a UV device, and relates to the technical field of cell research, and the method comprises the following steps: culturing cyanobacteria cells to a logarithmic phase to obtain a cyanobacteria sample; preparing an observation device; dropwise adding a cyanobacteria sample to the surface of the solidified culture medium, and air-drying and fixing; locally irradiating a sample area below the opening through a UV light source; sealing the sample through a cover glass and a second silicone rubber film sheet, and transferring the marking line to the cover glass; and observing the death process of cyanobacteria cells. Local precise UV stress of cyanobacteria protonema is realized by customizing a shading plate with a local opening, in-situ long-time stable observation is guaranteed by matching agar fixation and a sealed chamber design, and a continuous fluorescence microscopic imaging technology is combined; and the accurate visual tracking of the death time sequence, spatial distribution and death signal transmission process of different cells on the single protonema is realized.

This invention relates to a method for cultivating cyanobacteria to control protozoa, comprising the following steps: culturing cyanobacteria to the logarithmic growth phase to obtain a first microalgal culture; encapsulating the first microalgal culture in a polymer to obtain microalgal beads; inoculating the microalgal beads into a liquid culture medium for production cultivation to obtain a second microalgal culture; and when the second microalgal culture is contaminated by protozoa, separating the microalgal beads from the second culture and inoculating them into a liquid culture medium for production cultivation. This invention uses sodium alginate to encapsulate microalgae to control biocontamination, and the method also provides significant process advantages. Under static conditions, the rapid settling of the microalgal beads greatly simplifies biomass harvesting, eliminating the energy-intensive centrifugation step typically required for free cyanobacterial cells. This physical protection platform provides a scalable and environmentally sustainable solution to the persistent challenge of microbial contamination in large-scale cyanobacterial biofuel production.

The invention discloses a method for observing cyanobacteria cell communication by using an FRAP technology, and relates to the technical field of biological research, and the method comprises the following steps: culturing cyanobacteria; pretreating the cells; performing fluorescent dyeing and washing; fixing the sample; carrying out sample adaptation treatment; carrying out FRAP experiment observation; controlling an observation program; extracting and preprocessing data; processing and analyzing data; and verifying and applying a result. The problem of sample observation displacement is thoroughly solved through an innovative agar back-off fixation method, background fluorescence interference is eliminated by combining cell synchronization enrichment and multiple rounds of mild washing, and imaging and bleaching parameters adaptive to cyanobacteria light sensitivity characteristics are customized to balance signal quality and cell activity; meanwhile, a complete analysis process from sample adaptation and program automation control to data noise reduction calibration and nonlinear fitting is established, core parameters of communication dynamics are accurately extracted, and stable, accurate and quantitative observation of the communication dynamic process between cyanobacteria cells is achieved.

The present invention relates to a microorganism belonging to the genus Rhodococcus, the genus Xanthobacter, the genus Ancylobacter, the genus Shewanella, or the genus Aeromonas, and a method for producing the same, and more specifically, to a microorganism belonging to the genus Rhodococcus, the genus Xanthobacter, the genus Ancylobacter, the genus Shewanella, or the genus Aeromonas. The microorganism can be Rhodococcus hedyotidis (Rhodococcus cerevisiae) of the genus Rhodococcus, Xanthobacter flavus (Xanthobacter flavus) of the genus Xanthobacter, or Ancylobacter rudongensis of the genus Ancylobacter, and the microorganism can be a microorganism of the genus Ancylobacter, or a microorganism of the genus Ancylobacter, or a microorganism of the genus Ancylobacter, or a microorganism of the genus Ancylobacter and a microorganism of the genus Ancylobacter. The microorganism can be used for promoting the growth of blue algae, green algae, grey algae or euglena. The microorganism can be used to promote the growth of an organism belonging to the phylum cyanobacteria, unequal flagellum, euglenophyta, cryptophyta, rhodophyta, chlorophyta or chalaphyta.

This invention belongs to the interdisciplinary field of biomedicine and nanomaterials, specifically disclosing an engineered cyanobacteria-nanodiamond composite, its preparation method, and its applications. The composite includes genetically modified engineered cyanobacteria capable of stably synthesizing and secreting photosensitizers, and red fluorescent nanodiamonds containing nitrogen-vacancy centers that emit fluorescence at 600-750 nm under ≥532 nm light excitation. The nanodiamonds are loaded onto the surface of the cyanobacteria or into the extracellular matrix. This invention integrates the in-situ oxygen production / photosensitizer production function of cyanobacteria with the deep fluorescence emission characteristics of nanodiamonds, synergistically alleviating tumor hypoxia and enhancing deep photodynamic therapy. It exhibits good biocompatibility, controllable preparation, and broad application prospects in the treatment of solid tumors.

A method for converting a carbon source into ethylene glycol includes: providing a plasmid, in which the plasmid includes gene sequences of SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, and SEQ ID NO: 6; implanting the plasmid into cyanobacteria through an electroporation treatment, so that modified cyanobacteria are obtained; and providing the carbon source to the modified cyanobacteria, so that the modified cyanobacteria convert the carbon source into the ethylene glycol.

This invention discloses a primer and method for detecting cyanobacterial myotail phage. The specific primers designed for this invention target the nrdA sequence of the ribonucleotide reductase Alpha subunit gene of the T4-like group of marine cyanobacterial myotail phage, named nrdA-F / nrdA-R. nrdA-F is 5'-GATGACACCCTCGATAGYAT-3'; and nrdA-R is 5'-TGWGTRCARCATCKGACAGT-3'. The primers of this invention can cover the abundant and widely distributed cyanobacterial myotail phage in the ocean, making them suitable for rapid and accurate quantification of cyanobacterial myotail phage in marine environments, and have broad application prospects.

The application discloses a cyanobacterial nanogene carrier and a preparation method thereof. By irradiating cyanobacteria under appropriate wavelength light, toxic active oxygen is generated in the cyanobacteria, lysosomes are destroyed, and the gene carrier trapped in the lysosomes is released into the cytoplasm, so that the biological function of the gene can be better exerted, and the problems of the gene carrier in the prior art, such as cationic lipids, cationic polymers, difficult lysosome escape and low gene transfection efficiency, are overcome. Meanwhile, the preparation process is complex, other substances are easily introduced, and the safety is low, and the like are overcome, so that the application is more suitable for efficient delivery of the gene carrier, an effective treatment method is provided for treatment of tumors and the like, and the treatment of cancer and the like is beneficial.

The invention relates to the technical field of flocculant development, in particular to a bio-based flocculant and a preparation method thereof.The bio-based flocculant comprises 15%-25% of cationic polyacrylamide, 23%-57% of polymeric silicate aluminum ferric sulfate, 6%-10% of heavy metal composite adsorption aid powder, 13%-23% of a magnetic adsorption aid, 7%-13% of composite bacterial powder, 2%-5% of a microbial deodorant, 4%-9% of a microbial oxygen aid and 2%-16% of an excipient. The heavy metal composite adsorption assistant powder comprises stenotrophomonas maltophilia, providencia alcaligenes and a mixture of metallophilic bacteria and lysinibacillus, the composite bacterial powder is a mixture of agaricus blazei hyphae, lentinus edodes mushroom residues, flammulina velutipes mushroom residues and oyster mushroom residues, and the microbial oxygen assistant is a mixture of cyanobacteria chlorophycocyanobacteria and zoogloea. On one hand, the flocculation speed and the decomposition speed of heavy metal impurities in sewage can be effectively increased, on the other hand, magnetic adsorption and rapid flocculation of the heavy metal impurities in the sewage can be achieved, and meanwhile, the flocculant can also achieve increase of the oxygen content of the sewage, so that the oxygen exposure purification efficiency during sewage flocculation is effectively improved.

The present invention provides for a photosynthetic cell, such as a cyanobacteria cell, comprising a nucleic acid encoding a heterologous carbonic anhydrase (CA) operatively linked to a promoter capable of expression in the photosynthetic cell. The present invention also provides for a method for mineralizing carbon dioxide comprising: (a) culturing or growing a photosynthetic cell of the present invention in a medium under conditions such that the photosynthetic cell expresses the CA, (b) converting carbon dioxide into the H2CO3 through the enzymatic activity of the CA, (c) optionally complexing the carbonate of the H2CO3 with a metal to form a metal carbonate, (d) optionally precipitating the metal carbonate from the medium, (e) optionally removing or separating the metal carbonate from the medium and / or the photosynthetic cell.

The application discloses a cyanobacterium SCSIO17256 with high yield of exocellular polysaccharide and application of the cyanobacterium in anti-erosion of a coral island. The cyanobacterium is named as cyanobacterium Oculatella sp. SCSIO17256, and the preservation number is CCTCC NO: M2025524. The cyanobacterium Oculatella sp. SCSIO17256 provided by the application can effectively promote formation of a calcareous sand biological crust, has strong sand-fixing capacity, and has very strong shear resistance, and can be applied to prevention and treatment of water and soil loss of the coral island and construction of the calcareous sand biological soil crust of the coral island. Compared with physical sand-fixing, chemical sand-fixing and vegetation cultivation, the use of the microorganism as a new type of sand-fixing and cultivation crust has the advantages of strong adaptability, low cost, quick effect, etc.