The invention discloses purified cotton knitted fabric having a soft hemp effect and a manufacturing method thereof, and belongs to the field of textile fabric. Purified cotton yarns and hemp knot yarns which are made of purified cotton in a spun mode are woven together to form the purified cotton knitted fabric having the soft hemp effect, the purified cotton yarns are high-count cotton yarns, and the hemp knot yarns are purified-cotton high-twist hemp knot yarns. Weaving processes are designed according to performance of the two kinds of yarns, so that the purified cotton knitted fabric having the soft hemp effect integrates good characteristics of the two kinds of yarns, has stiff hand feeling, is smooth and has the appearance of hemp materials. Meanwhile, the defects that natural fibrilia fabric fluffs easily, is poor in creasing recovery capability, makes skin itch and the like are overcome, and the purified cotton knitted fabric having the soft hemp effect is good in drapability and excellent in air permeability, and has no stimulation on the skin. The water absorption ratio, the water vapor permeability, the wicking height, the dropping water diffusion time and the evaporation rate of the purified cotton knitted fabric having the soft hemp effect are respectively better than those of ramie fabric by 25%, 22%, 55%, 16% and 18%, and the purified cotton knitted fabric having the soft hemp effect is excellent in performance.