The invention relates to the field of medicine, in particular to a preparation process of an Eddy injection preparation. The preparation process of the Eddy injection preparation comprises the following steps: taking Chinese blister beetle, ginseng, astragalus membranaceus and acanthopanax as raw materials, performing warm dipping on the ginseng and performing alcohol extraction, jointly decoctingthe ginseng residues, the astragalus membranaceus and the acanthopanax and performing aqueous extraction, combining extraction liquid, performing alcohol and water precipitation alternately for manytimes and performing refrigeration treatment so as to completely precipitate and remove the impurities, greatly reduce the impurities in the medicine liquid and reduce the occurrence rate of adverse reaction, adjusting the pH, performing ultrafiltration and performing refrigeration for later use; performing warm dipping on the Chinese blister beetle and performing aqueous extraction, performing treatment by a lime surface method, performing alcohol precipitation for twice, refrigerating, filtering, performing water precipitation for twice, adjusting the pH, refrigerating and performing ultrafiltration; and mixing the Chinese blister beetle extracting liquid, the extracting liquid of the ginseng, the astragalus membranaceus and the acanthopanax, adding auxiliary materials and adjusting thepH. The preparation process of the Eddy injection preparation has the advantages of high extraction efficiency, high purity and low occurrence rate of adverse reaction.