The invention discloses a welding and positioning device for lamp strips and lamp beads. The welding and positioning device comprises a device main body, a sliding rail is arranged in the interior of the device main body, a lamp bead fixing seat is slidably mounted on the sliding rail, a lamp bead adsorption device used for limiting the positions of the lamp beads is arranged at a position, close to the bottom end, in the interior of the device main body, and a placement opening is formed in the outer surface of the upper end of the device main body. According to the welding and positioning device for the lamp strips and the lamp beads, welding can be directly carried out, people do not need to manually maintain the positions of the lamp beads and the lamp strips during welding, and therefore people can conveniently weld the lamp beads to the lamp strips, the welding efficiency and the success rate are improved, the plurality of sets of lamp beads are welded at a time, the welding speed is high, the stability of the lamp beads can be kept, the lamp beads are prevented from shaking in the welding process, the yield can be improved, the position of the lamp strip fixing plate can be limited, the positions of the lamp strips can be further limited, people can conveniently complete welding of the lamp strips and the lamp beads, and better use prospects are brought.