The present invention relates to a TPEE modified thermoplastic elastomer automobile sealing strip. The sealing strip is prepared from the combination of a binder and an addition agent, wherein the binder is composed of the combination of PP and two thermoplastic elastomers, the PP is a resin, and the two thermoplastic elastomers are a polyene elastomer TPE-A and a polyester elastomer TPE-B. The preparation method comprises the steps that 1) automatic compounding, plastication, oil charge and pre-dispersion are carried out; 2) twin-screw plastication, liquation, compounding, extrusion and granulation are carried out; 3) product inspection of a sealing strip material is carried out; 4) extrusion processing of the sealing strip is carried out, and the processes of feeding, compounding, and underwater traction and extrusion are completed on a sealing strip extrusion processing production line; 5) finished strip inspection of the sealing strip is carried out, inspections of structural, physical and chemical properties of the sealing strip are carried out; 6) cutting and jointing are conducted on the sealing strip, according to the specification requirement of the sealing strip, cuttingis carried out, and a jointing mold is used for jointing; 7) packing and storage are carried out, the finished product of the sealing strip is tested again, and the qualified product is put into a warehouse. The TPEE modified thermoplastic elastomer automobile sealing strip has the advantages that the performances are excellent, the softness and hardness of the product are adjusted by PP content,the application range is wide, and the free lifting of the automobile sealing strip at an ultra-low temperature can be met.