The invention discloses a sulfonic acid tetraphenyl porphyrin nano-fluorescent material and a preparation method thereof, which relate to a fluorescent porphyrin material and a preparation method thereof. The sectional shape of the material is of a rectangular sheet or strip or sheet and strip. The method comprises the following steps: preparing a TPRS alcohol solution first, preparing a TPPS nano-fluorescent material dispersion system, and obtaining a product through separation and drying. The material has smaller size and better photosensitivity, has good characteristics of a porphyrin monomer such as rigidity and flexibility, electronic buffer capacity, photosensitivity, high chemical stability, wide spectral response, and also has special properties after the nanometer treatment such as small-size effect, surface effect, quantum effect, interfacial effect. The method is simple, has low production cost, and is convenient for promotion and application. The material prepared by adopting the method can be widely used as markers, photosensitive materials as well as sensitive recognition elements and materials for constructing photoelectric devices, molecular devices and sensors.