The invention discloses a device assembly for horizontal well temporary well plugging operation through coiled tubings and a use method. According to the main points of the technical scheme, the device assembly comprises a coiled tubing vehicle, and a single-machine well cementation vehicle or a double-machine well cementation vehicle. The coiled tubing vehicle comprises a vehicle head, a control cabinet, a power system, a roller, the 2-inch coiled tubing, the 2.375-inch coiled tubing, an injection head and corresponding parts, wherein the injection head and the corresponding parts are connected to the 2.375-inch coiled tubing. The single-machine well cementation vehicle or the double-machine well cementation vehicle comprises a vehicle head, a power system, an instrument control system and a metering liquid tank. The coiled tubing vehicle is matched with the single-machine well cementation vehicle or the double-machine well cementation vehicle for the temporary well plugging operation process including the steps of well tripping, bridge plug running, continuous cement plug injection and reverse circulation well flushing. By adoption of the device assembly and the use method, one-time continuous cement plug injection (the vertical thickness is larger than 200 m, and the along hole depth length is larger than 300 m) for a horizontal well can be achieved, the construction period is greatly shortened, the process complexity degree is lowered, the well control safety risk is reduced, and operation cost is saved.