The invention relates to an ultrahigh vacuum system, in particular to an ultrahigh vacuum acquisition system and method for experimental preparation of ultra-cold atoms and molecules, and solves a problem that the vacuum degree of an existing ultrahigh vacuum system is not high enough. The ultrahigh vacuum acquisition system for experimental preparation of the ultra-cold atoms and molecules comprises a first vacuum chamber, a second vacuum chamber, a third vacuum chamber, a fourth vacuum chamber, a first titanium sublimation pump, a second titanium sublimation pump, a first sputtering ion pump, a second sputtering ion pump, a third sputtering ion pump, a first angle valve, a second angle valve, a first turbo molecular pump, a second turbo molecular pump, a first ion gauge, a second ion gauge, a third ion gauge, an inflation valve, a nitrogen source, an alkali metal source, a first gate valve, a second gate valve, a Zeeman slower and a three-way pipe. The system and the method are applicable to experimental preparation of the ultra-cold atoms and molecules.