The invention provides a helium ionization detector and a preparation method thereof. The helium ionization detector comprises a substrate, an excitation electrode pair, gas inlet pipes, a bias electrode and a collection electrode; a cavity is formed in the substrate, the cavity comprises an excitation electrode channel, an air inlet channel and an air outlet channel, the excitation electrode channel and the air outlet channel are communicated to form a first intersection area, and the air inlet channel and the air outlet channel are communicated to form a second intersection area; an excitation electrode region of the excitation electrode pair is positioned in the first intersection area; the gas inlet pipes are a first gas inlet pipe and a second gas inlet pipe which have the same morphology and are oppositely arranged, and can provide auxiliary carrier gas and sample carrier gas with the same pressure intensity for the second intersection area; the bias electrode and the collection electrode are located in the air outlet channel and arranged on the outer side of the second intersection area away from the first intersection area. According to the invention, a high-pressure quasi-zero flow velocity region can be formed in the cavity, helium plasma excitation is facilitated, the helium flow velocity in the area is extremely low, helium plasma groups can be stabilized, and then baseline noise can be reduced.