The invention provides a biochemical joint preparation method and application of absolute 
rose oil. The preparation method comprises the steps that coarse rose 
powder is prefermented by beta-
glucosidase, 
amylase and 
cellulase to generate 
aroma, 
reflux extraction is conducted for 3-5 h with an 
aqueous solution the pH value is regulated to be 9-14, a 
Maillard reaction is conducted for 3-5 h, suction 
filtration is conducted, filtrate is retained, an 
ethanol solution is added into filter residues for secondary 
reflux extraction, suction 
filtration is conducted, filtrate is retained, the filtrates obtained in two times of suction 
filtration are mixed, concentration and 
alcohol precipitation are sequentially conducted, and a 
rose extract is obtained; the 
rose extract is purified through molecular 
distillation, light components are retained, heavy components are purified through molecular 
distillation again, the light components are mixed, and the purified absolute 
rose oil is obtained. The absolute 
rose oil has purer and richer 
aroma and fewer impurities, and the 
irritation is reduced. When the absolute rose oil is applied to reconstituted tobaccos, the color and luster of the reconstituted tobaccos is more natural and uniform, the 
aroma can be enriched, 
irritation and offensive 
odor can be reduced, and the sensory quality can be improved; when the absolute rose oil is applied to a novel 
tobacco product atomizing agent, the aroma is enriched, the noble rose note is improved, and the tobacco aroma is increased.