The invention discloses a human placenta tissue fluid injection and a preparation method of the human placenta tissue fluid injection. A placenta tissue is subjected to freeze-drying treatment and secondary pulverization to obtain placenta tissue dry powder, the problem of toxin and bacteria breeding caused by the fact that the placenta tissue is prone to deterioration in the conventional heatingand drying process is prevented, meanwhile, acid hydrolysis, centrifugal separation and the like are combined, and acid-hydrolyzed placenta tissue fluid is obtained. Compared with conventional repeated freezing, thawing, filtration and the like, the purifying efficiency is improved, further, adsorption and elution are carried out through a protein adsorption column, purer active peptide is obtained, and the purity of the active peptide is improved. The preparation method is simple in steps, easy to popularize in industry and high in application value. In addition, the protein adsorption columnis used for adsorption and elution, the content of active substances in the obtained human placenta tissue fluid is higher, use is more convenient in medical treatment, the human placenta tissue fluid can be subpackaged to prepare human placenta tissue fluid injections with different concentrations respectively, and use is more flexible and more convenient.