The invention discloses a seasoning for salting beef. The seasoning for salting the beef comprises the following raw materials in parts by weight: 5 to 10 parts of ginger powder, 5 to 10 parts of garlic powder, 3 to 6 parts of a yeast extract, 3 to 5 parts of a lemon extract, 15 to 25 parts of traditional Chinese medicinal powder, 5 to 15 parts of proteolytic enzyme, and 1 to 5 parts of a fresh strengthening agent. The seasoning for salting the beef is obtained by mixing the raw materials in proportion. A using method comprises the following steps: cleaning the beef; mixing 20 to 30 g of the seasoning and 50 to 100 g of water uniformly, wherein 20 to 30 g of the seasoning is used for 500 to 1500 g of the beef; coating the beef with the mixture; salting for 10 to 15 minutes, and performing the cooking processes, such as stewing, boiling, deep frying and frying. The seasoning for salting the beef disclosed by the invention does not contain the pungent tastes of spiciness, piquancy, sourness and the like; the beef is easy to cook thoroughly, but the sense of strength and the freshness cannot lose along with the prolongation of time; the prepared beef is fresh without special smell, and is delicate in taste.