The invention relates to a preservative, in particular to a traditional Chinese medicine preservative for a fish stripping specimen and a preparation method of the traditional Chinese medicine preservative. The method comprises the following steps: cleaning five traditional Chinese medicinal materials, namely rhubarb, coptis chinensis, astragalus membranaceus, falangal and fructus forsythiae; baking at 55-65 DEG C for 45-50 hours, and crushing until the granularity is below 40mesh; weighing all traditional Chinese medicines, putting the weighed traditional Chinese medicines into water, heating in a water bath at 55-65 DEG C for 10-14 hours, and then adding alums; or weighing all traditional Chinese medicines, putting the weighed traditional Chinese medicines into water, decocting into a paste with a slow fire, and then adding the alums; and adding p-nitrophenol to 95wt% ethyl alcohol, pouring the mixture into the medicinal materials in the step (2), stirring into a paste and sealing. The traditional Chinese medicine preservative for the fish stripping specimen has a good broad-spectrum bactericidal effect, is slow to release, nontoxic, healthy, and free of harm to bodies of preparation staff and users, and is capable of ensuring the preservative effect of the specimen for a long period of time; and the preparation method is simple and convenient, low in cost, relatively few in required equipment, and easy to popularize.