The invention discloses a construction process of a high-pressure stainless steel acetylene filled tube. The process comprises following steps of firstly, cutting, discharging and numbering of a mother tube; secondly, groove preparation; thirdly, sub tube discharging according to the mother tube; fourthly, sub tube outer burr grinding; fifthly, sub tube filling; sixthly, sub tube leveling; seventhly, sub tube inner grinding; eighthly, filling, shaping and sub tube spot welding; ninthly, assembling of straight tube segments; ninthly, pipe fitting and flange assembling; eleventhly, pipe fittingpadding filling; twelvethly, pipefitting industrialization prefabrication. According to the process, the straight pipe segments are wholly filled, a filling platform is developed, a whole filling method is adopted for construction, and the problems that due to one-by-one sub tube filling, the efficiency is low, and the quality is poor are solved; sub tube whole filling is carried out, after filling, sub tube arranging interval quality is good, staggered collapsing is avoided, the schedule is greatly improved, and the traditional straight pipe segment filling construction process is thoroughlychanged.