130 results about "Bacteriome" patented technology

Described are methods and antibodies useful for reducing, eliminating, or preventing infection with a bacterial population in an animal. Also described herein are antigens useful for targeting by heavy chain antibodies and VHH fragments for reducing a bacterial population in an animal.

The invention discloses a preparation method of a biochar-loaded synthetic microbial agent and application of the biochar-loaded synthetic microbial agent in plant growth promotion and soil remediation, and relates to the technical field of plant growth promotion and soil remediation. The invention aims to solve the problems that the existing single degradation strain does not have the broad-spectrum PAEs degradation capability and the degradation effect of the single degradation strain on PAEs in indigenous bacterial communities is unstable. The synthesized microbial agent constructed by the invention shows more excellent performance on degradation and utilization of DBP, and the 48-hour degradation rate of the synthesized microbial agent on DBP is greater than the degradation rate of the synthesized microbial agent when only DNB-S1 degrading bacteria exist. The pores of the biochar provide a suitable environment for the habitat of microorganisms, so that the damage of high-concentration DBP to bacteria is avoided, the DBP adsorbed on the biochar is promoted to be utilized by DNB-S1, and the chemotaxis of bacteria is facilitated. The invention can obtain the preparation method of the biochar-loaded synthetic microbial agent and the application of the biochar-loaded synthetic microbial agent in plant growth promotion and soil remediation.

The invention discloses a bacillus megaterium Q7 for promoting the growth of leucaena leucocephala and improving the abundance of a probiotic microbial population. The bacillus megaterium Q7 is a bacillus megaterium for promoting the growth of leucaena leucocephala. Belongs to the technical field of microorganisms. The bacillus megatherium Q7 provided by the invention improves the conversion from total-effect nutrients to effective nutrients, and enhances the consumption of the leucaena leucocephala on soil nutrients, thereby achieving the effect of promoting the growth of the leucaena leucocephala. After the bacillus megatherium Q7 provided by the invention is applied, the enzyme activity of soil is remarkably improved, the conversion of available nutrients in the soil is remarkably enhanced, and the nutrient consumption capacity of leucaena leucocephala is enhanced. The bacillus megaterium Q7 is applied to promote the growth and development of the leucaena leucocephala, so that the contents of soil hydrolytic nitrogen and available phosphorus are remarkably increased, and the conversion to soil nutrients is enhanced. In the genus level, the average relative abundance of bacillus treated by the bacillus atrophaeus Q7 is increased to 9.97% from 1.33%, the bacillus pseudobecomes the leucaena leucocephala soil community dominant bacteria genus, and the relative abundance of Fictibacillus is increased by about 6 times, which indicates that the inoculation of the strain Q7 enables the leucaena leucocephala soil community structure to be changed, and the leucaena leucocephala soil community dominant bacteria genus is changed. And the increase of the relative abundance of dominant bacteria is promoted. Wherein the bacillus and the Fictibacillus belong to obvious increase of relative abundance and are possibly an important factor for promoting plant growth.

A method for improving gut microbiota population in a subject by administering a composition comprising allulose, erythritol or a combination thereof. A method for increasing one or more colonic bacterial populations from the families Lachnospiraceae or Eubacteriaceae by administering a composition comprising allulose, erythritol or a combination thereof. A method for preventing, treating or ameliorating a disease in a subject by administering a composition comprising allulose, erythritol or a combination thereof. The disease may be one or more of metabolic syndrome, obesity, type 2 diabetes, insulin resistance, hyperlipoproteinemia, hyperuricemia, hepatic steatosis, hypercholesterolemia, hypertriglyceridemia, irritable bowel syndrome (IBS), colon cancer, allergy, non-alcoholic fatty liver disease, inflammatory bowel disease, cardiovascular disease, or inflammation. The bacterial population may be the species Anaerostipes hadrus, Blautia obeum, Anaerobutyricum hallii, Eubacterium rectale (Agathobacter rectalis), Eubacterium callanderi, Eubacterium limosum and Eubacterium maltosivorans. A composition comprising allulose, erythritol or a combination thereof and one or more colonic bacterial populations from the families Lachnospiraceae or Eubacteriaceae is also provided. The composition may in the form of a food composition.

Provided are compositions and methods for selectively reducing the amount of antibiotic resistant and / or virulent bacteria in a mixed bacteria population, or for reducing any other type of unwanted bacteria in a mixed bacteria population. The compositions and methods involve targeting bacteria that are differentiated from other members of the population by at least one unique clustered regularly interspaced short palindromic repeats (CRISPR) targeted DNA sequence. The compositions and methods can be readily adapted to target any bacteria or any bacteria plasmid, or both.

The invention discloses mortierella alpina GRB-1, a fungicide and application of the fungicide, and belongs to the technical field of agricultural microorganisms. The Mortierella alpina GRB-1 is separated from root systems of healthy fruit trees in a continuous cropping apple orchard for the first time, and the Mortierella alpina GRB-1 has the capabilities of producing protease, producing siderophores, dissolving phosphorus, producing ammonia gas and producing auxin, can inhibit fusarium and has the application potential of relieving continuous cropping obstacles. The product prepared by combining the straw with crop straws has an excellent effect on relieving apple continuous cropping obstacles, can promote the growth of overground parts and underground parts of continuous cropping apple trees, improves the relative abundance of bacterial communities in apple continuous cropping soil and the nutrient content in the apple continuous cropping soil, improves the root activity of apples, and improves the survival rate of the apples. The method can be used for preventing and treating apple successive cropping obstacles.

The invention relates to the field of intelligent regulation and control, and discloses a factory recirculating aquaculture intelligent control method and system fusing multi-source data. The method comprises the following steps: acquiring environmental chemical index data and microbial activity signal data to obtain a comprehensive real-time monitoring data set; if the dynamic change characteristic of the bacterial community structure indicates that the conversion rate is lower than a conversion threshold value, analyzing the coupling influence of an oxygen supply amount index and a nutrient solution proportion index to obtain an optimized adjustment parameter set; adjusting the balance between the nutrient solution dosage index and the buffer agent dosage index, and determining a final regulation and control scheme; according to the stable output value and the adjustment instruction, fusing the dynamic change characteristics of the bacterial community structure, processing real-time feedback circulation, and determining a system stable state index; and updating the bacterial activity model, judging whether the conversion efficiency reaches a target or not, and obtaining an optimized iteration signal. The problems that an existing system is difficult in interactive analysis, low in regulation and control accuracy, weak in data fusion and unstable in conversion efficiency due to state updating lag are solved.

The invention relates to a river planktonic bacterial community composition prediction method based on an artificial neural network auto-encoder, and belongs to the field of environmental science. According to the method, an original data set is formed by collecting river planktonic bacteria OTU relative abundance and related water environment, weather and other data, and preprocessing is carried out; a potential space based on OTU composition and a potential space based on environmental elements are constructed through a training set by using an auto-encoder model, the difference between the two potential spaces is minimized in the training process, and the OTU composition is obtained from the potential spaces through the same decoder. An SHAP value interpretation model is utilized to understand how each environmental element affects model prediction of a single sample, and the overall influence of the environmental elements on the model prediction is understood by averaging SHAP values of a plurality of samples. According to the method, the change relationship between various environmental elements and the relative abundance of the river planktonic bacteria is explored from the perspective of a data-driven deep learning model.

The invention provides a method for evaluating river pollution and application thereof, and belongs to the technical field of river pollution treatment. The invention discloses the relationship between the microbial community composition change and the environmental factor in the river water body of the drainage basin. According to the invention, bacterial communities and virus communities in a river are significantly related to environmental factors (r is greater than 0.8, and p is less than 0.05), which indicates that the environmental factors are the main reason influencing the difference and abundance change of the bacterial communities and virus communities in the river, and environmental pollution control measures should be taken to establish a perfect pollution control system. According to the method, a scientific basis is provided for the management of a river ecosystem and the protection of biological diversity, the influence of the environmental factors on the point location can be known by detecting the microbial community structure of the river in the river, a reference is provided for reasonably utilizing scarce fresh water resources, and a safety guarantee is provided for preventing and controlling the water environment pollution risk and ensuring the water quality safety.

This application relates to methods and materials for providing a benefit to a seed or seedling of an agricultural plant (e.g., an agricultural grass plant), or the agricultural plant derived from the seed or seedling. For example, this application provides purified bacterial populations that include novel seed-origin bacterial endophytes, and synthetic combinations of seeds and / or seedlings (e.g., cereal seeds and / or seedlings) with heterologous seed-derived bacterial endophytes.

The invention discloses a rare-earth-based bionic nano material as well as a preparation method and application thereof, and relates to the field of microbial pollution and anti-corrosion treatment. According to the invention, the active center of lactonase can be simulated by using a zinc-based material, and ester bonds in N-acyl homoserine lactone (AHL) molecules can be hydrolyzed by using the lactonase so as to block bacterial quorum sensing (QS) among bacterial florae, so that the antibacterial effect is achieved. Meanwhile, the outer layer of zinc is provided with a cerium element as a shell, so that on one hand, permeation of oxygen, moisture and corrosive ions can be prevented; on the other hand, the structure has hydrophobicity, and corrosion of moisture and salt ions to a metal matrix is further reduced through the hydrophobicity. Cerium and an oxide thereof have certain antibacterial ability and can have a synergistic effect with zinc, Ce can promote generation of Zn-N and generate strong electron interaction, better hydrophobicity is shown, and attachment and growth of microorganisms on the metal surface can be further inhibited.

The present application relates to the field of sanitary products, and provides a high-soft skin-friendly sanitary napkin and a preparation method thereof, which solves the problem that the existing sanitary napkin has poor adhesion effect of bacteriostatic components, cannot specifically inhibit harmful bacteria in the vagina, promote the dominant position of beneficial bacteria in the vagina in the bacterial community, does not have the function of releasing bacteriostatic components under specific conditions, cannot have high-efficiency bacteriostasis while reducing the long-time contact of bacteriostatic components with the skin of the vagina, and has a problem of high cytotoxicity; the sanitary napkin comprises, from top to bottom, a surface layer, a flow guide layer, a core body and a leakage-proof bottom film, the surface layer is modified pure cotton non-woven fabric, and the modified pure cotton non-woven fabric is obtained by rolling and drying after immersing pure cotton non-woven fabric in bacteriostatic component microcapsule mixed solution.

A synthetic microbial community (SynCom) agricultural crop fertilizer composition comprises a microbial stabilizing buffer and bacterial strains configured to mimic the natural diversity of the bacterial community found in the crop phyllosphere microbiome, but at a reduced complexity, yet sufficient to effectively provide increased plant productivity and / or disease protection.

The invention discloses a growth-promoting methylobacterium R5018 strain and an application of the growth-promoting methylobacterium R5018 strain. The strain R5018 is obtained by separation from a rice phyllospheric microflora and methanol domestication and screening, and is preserved in the China Center for Type Culture Collection (CCTCC), and the preservation number is CCTCC NO: M20252544. The rice growth-promoting microbial inoculum prepared from the strain can be stably colonized at phyllosphere and promote the growth of overground and underground parts of rice through seed soaking, foliage spraying or combined application of the seed soaking and the foliage spraying, and the ear number, the grain number and the yield are remarkably increased. Compared with the prior art, the strain disclosed by the invention is derived from a rice phyllospheric bacterial community, is high in adaptability, environment-friendly and efficient, provides a new microbial technical scheme for high-yield cultivation of rice, and has a wide popularization and application prospect.

Methods for improving the gut microbiota of a subject, increasing one or more bacterial communities belonging to the Helicobacterium or Euglobulaceae families in their colonic flora, for the prevention, treatment, or relief of disease, and for increasing the level of short-chain fatty acids in the colon, comprising administering to the subject a composition containing allulose, erythritol, or a combination thereof. The composition used in the above methods comprises allulose, erythritol, or a combination thereof. The composition comprises allulose, erythritol, or a combination thereof, and one or more colonic bacterial communities from the Helicobacterium or Euglobulaceae families.

A method for detecting anxiety and / or depression metabolic and microbiome markers in a patient with irritable bowel syndrome (IBS) is provided. The method begins with obtaining a serum sample and a fecal sample from the patient. Both samples are processed to extract a gut metagenome, including a bacteriome, a mycobiome, and a virome of the patient, and metabolic features. A classifier is utilized to detect whether a marker set of microbial species and metabolites for depression and anxiety is present in the gut metagenome and the metabolic features.

This invention relates to the field of soil treatment technology, specifically a method and its application for reducing compound fungicides in soil-plant systems by combining nano-silica and nitrification inhibitors. Nitrification inhibitors are used as a nitrogen management measure, and the application of nanomaterials influences soil abiotic properties and bacterial communities to drive the degradation of paclobutrazol and carbendazim. The application of nitrification inhibitors (DCD) produces multiple effects: significantly reducing carbendazim residues in soil-plant systems, paclobutrazol residues in soil, and NO3 in plants. ‑ -N and promote the content of soluble sugars and proline in plants. DCD reduces the activity of the soil electron transport system and β-glucosidase activity, while the addition of nano-SiO2 can mitigate this negative effect. Nano-SiO2 selectively enhances the performance of DCD in reducing compound fungicide residues in pak choi and reducing paclobutrazol residues, but hinders the degradation of carbendazim in pak choi.

The invention relates to the technical field of microbial compositions, in particular to a microbial composition for preventing and treating citrus canker and application of the microbial composition. According to the microbial composition provided by the invention, the pseudomonas Qingdao microbial agent, sodium lignin sulfonate, trehalose, glutathione, ascorbic acid, lecithin and trace elements (Zn, B and Mn) are taken as main raw materials, and the effects of efficiently inhibiting bacteria, remarkably inducing and improving the activity of key defensive enzymes of leaves, promoting synthesis and accumulation of disease-resistant substances and improving the disease resistance of the leaves are achieved through the matching effect of all the components. The diversity and richness of citrus phyllospheric bacterial communities are improved, and the purpose of constructing a stable community dominated by beneficial bacteria is achieved. In other words, the basic stress resistance of the plants can be enhanced, and meanwhile, the growth and metabolism activity of beneficial bacteria is also promoted, so that effective, eco-friendly and sustainable prevention and control of the citrus canker are achieved.

This disclosure provides a method of treating gastrointestinal inflammation such as inflammatory bowel disease (IBS) with a bacteriophage containing composition. The bacteriophage containing composition can be used as prebiotic supplementation and support of the gastrointestinal microflora. This disclosure relates to compositions that comprise one or more types of bacteriophage and methods of using such bacteriophages as a prebiotic to promote the growth of beneficial bacteria by decreasing harmful bacterial populations and releasing nutrients into the environment for good bacteria in the digestive system of an individual.

Provided herein, inter alia, are compositions of organic acid-producing microorganisms and methods of making and using the same to inhibit pathogenic bacterial populations in the gastrointestinal tracts of an animal and additionally promote improvement of one or more metrics in an animal, such as increased bodyweight gain, decreased feed conversion ratio (FCR), improved gut barrier integrity, reduced mortality, reduced pathogen infection, and reduced pathogen shedding in feces.

The present application provides a kind of planting method of reducing allelochemicals and improving polysaccharide content of Rhizoma Polygonati, belongs to plant cultivation technical field.The planting method is planted in acidic sandy loam soil, and "biochar C" is applied to the soil 1-2 times in September-October of the second and third years, and the fruits mature, the stems are laid down after the third year, and then it can be harvested."Biochar C" is a modified, loaded with salicylic acid and UDP-D-galactose biochar.The present application not only helps to improve the physicochemical properties and bacterial community diversity of Rhizoma Polygonati planting soil, thereby reducing the allelochemicals content of Rhizoma Polygonati rhizome, but also can improve the polysaccharide content of rhizome, which helps to improve the quality of Rhizoma Polygonati and enhance its efficacy.

The present invention relates to the preparation and use of high-entropy nanozymes with multi-pathway synergistic antibacterial effects, belonging to the field of nanozyme catalytic materials. The present invention, for the first time, utilizes a hydrothermal method and high-temperature calcination method to synthesize oxygen-vacancy-rich high-entropy oxides with haloperoxidase-like, peroxidase-like, and glutathione oxidase-like activities. These oxides can catalyze bromide ions and hydrogen peroxide to generate hypobromous acid, which interferes with bacterial quorum sensing, and hydroxyl radicals, which disrupt structure and function. Furthermore, these oxides consume glutathione, which maintains the internal redox balance of bacteria. This allows for multi-pathway synergistic and efficient bacterial damage and inactivation, helping to address the problems of existing antifouling nanozyme materials, which suffer from a single catalytic pathway and poor component adjustability. This allows for the practical expansion of high-entropy nanozyme coatings, and has potential application prospects in the field of marine biofouling.

A lysogenic phage community, its extraction method and application. The tangential flow technology is used to proportionally isolate bacteria and phage communities from in-situ soil, and single-bacteria communities and single-phage communities are obtained through enrichment and concentration. At the same time, mitomycin C is added to the single-bacteria community for lysogenic induction, and then the tangential flow system is used again for filtration and enrichment to obtain a high-throughput lysogenic phage community. Finally, we obtain single-bacteria communities, bacteria-phage mixed communities, and bacteria-lysogenic phage mixed communities. The phage transplantation technology can promote the participation of indigenous microorganisms in the process of promoting plant growth and development. The transplanted lysogenic phages can participate in the synthesis and metabolism of host plant hormones by optimizing the microbial network structure and injecting auxiliary metabolic genes. This method provides a long-term and environmentally friendly microbial synergistic growth promotion technology for the development of green agriculture in China.

A method and system for increasing beneficial bacteria populations in a subject's oral cavity and decreasing pathogenic bacteria to address a variety of diseases and conditions, thereby enhancing a person's oral and overall health, including through the use of oral strips that adhere to surfaces in the oral cavity and that include at least one of xylitol, Lachnospira, Veillonella, Faecalibacterium and / or Rothia bacteria, including bacteria transformed via a CRISPR system. Certain embodiments employ light to modify oral cavity bacterial populations, including the use of bioluminescent oral strips that promote the growth of beneficial bacteria and decrease pathogenic bacteria populations.

The invention features E. faecalis phage-derived antimicrobial agent (efagins). The invention also features compositions and methods of using the efagins to treat bacterial infections.

The present invention belongs to the technical field of the restoration of coal mine restoration areas. In order to understand the effects of heavy metals and PAHs on the diversity of restored plants and soil microbial communities under different utilization modes, a method for constructing a combined plant-microbial community restoration system in coal mine restoration areas is provided. The quadrat survey method is used to investigate the composition and structural characteristics of the vegetation community in the gangue restoration area; the Miseq high-throughput sequencing technology is used to detect the composition and diversity of the bacterial community in the soil of the gangue area, analyze the composition, distribution and diversity characteristics of the microbial community at different stages of mining area accumulation and restoration and in different seasons of the restoration area, and analyze their differences, and use the co-evolution strategy to screen the plant rhizosphere-microbial ecological community that can efficiently degrade pollutants; construct a soil-plant-microbial efficient degradation system. The plants that can enrich heavy metals and have a strong nitrogen fixation effect are screened out, and the dominant bacterial genera that are tolerant to heavy metals or can degrade PAHs are found, which helps the further restoration of the gangue mountain in this area.

An anaerobic chamber system to evaluate human enteric disease is described herein that can be used to test therapeutic components. In specific embodiments, the anaerobic chamber is used to determine the effect of one or more bacterial communities on ex vivo enteroid cultures. In one application, the anaerobic chamber system is used to determine the efficacy of therapeutic components in ameliorating human enteric disease using personalized medicine.

This invention discloses the application of α-bisabolol in the preparation of a quorum sensing quencher for Serratia liquefaction. It belongs to the field of pharmaceutical technology. The purpose of this invention is to provide an application in the preparation of a quencher for inhibiting the quorum sensing system of Serratia liquefaction, thereby reducing the toxicity of pathogenic bacteria without inhibiting their growth. This invention provides an application of bisabolol in the preparation of a quencher for inhibiting the quorum sensing system of Serratia liquefaction. It offers a novel solution for developing efficient and safe food preservatives.

The present invention discloses a strain of Mortierella alpina GRB-1, a microbial agent and its application, belonging to the field of agricultural microbiology. The present invention is the first to isolate a strain of Mortierella alpina ( Alpine Mortierella ) GRB‑1, a strain that simultaneously produces proteases, siderophores, phosphate solubilization, ammonia, and auxin, can inhibit Fusarium spores and has the potential to alleviate continuous cropping problems. Combining it with crop straw to create a product has shown excellent results in alleviating continuous cropping problems with apples. It can promote the growth of both above- and below-ground parts of continuously cropped apple trees, increase the relative abundance of bacterial communities and nutrient content in soils containing continuously cropped apples, and enhance apple root vitality, making it suitable for preventing and treating continuous cropping problems.

A method and system for predicting a community dynamic change of a long-period enriched bacterial community in a cold seep. The method includes: collecting Raman spectra of a bacterial culture sample at different enrichment stages, analyzing them using an MCR-ALS algorithm to acquire metabolite data, and storing the metabolite data, environmental parameters, bacterial community α-diversity, and relative abundances of key bacterial taxa jointly as an original dataset; performing importance calculation and data screening on the original dataset using a CART decision tree algorithm; training a random forest prediction model for prediction using the screened dataset; and finally performing community dynamic change prediction using a trained random forest model. According to the present disclosure, a dataset is formed through routine sampling and collection of a complete bacterial community succession process in an early stage of enrichment culture, and an accurate prediction model is constructed by using a machine learning algorithm.