The invention discloses a virus protection biological safety cabin for a living body fluorescence imager, comprising: a cabin body assembly which comprises a box body, a sealing upper cover, an air inlet high efficiency filter, an air outlet high efficiency filter, an air inlet one-way valve, an air outlet one-way valve and a constant temperature plate; an internal electronic module, which comprises a control chip, a first battery, a microswitch and an air pressure sensor; the external electronic module comprises a first display screen, a buzzer, a second battery and a first switch; and an air pump module. By arranging the external electronic module, the air pressure value of the box body can be displayed in real time, and the air pressure abnormity alarm function is achieved; meanwhile, the sealing structure between the sealing upper cover and the box body is optimized, the sealing performance can be greatly improved, harmful gas can be prevented from leaking from the joint of the sealing upper cover and the box body, the sealing requirement can be met by adopting a single-layer bin body structure, and therefore the device structure can be simplified, the size of the whole device can be reduced, and operation is easier and more convenient.