The invention relates to an on-line double-sided automatic thickness measuring device of organic glass, which mainly comprises a control system, a machine frame, a support frame, a detection frame, a cross beam, a cross beam regulator, a feeding distance measuring device and a plurality of thickness measuring devices, wherein the upper end of the machine frame is fixedly connected with the detection frame, the cross beam is connected onto the upper edge frame of the detection frame through the cross beam regulator, the inside of the detection frame is fixedly connected with the support frame, a plurality of support guide shafts are arranged on the support frame, a feeding speed measuring device is arranged at one end of any support guide shaft for measuring the feeding distance of an organic glass plate, the thickness measuring device comprises upper thickness measuring units and lower thickness measuring units, and a plurality of upper thickness measuring units and lower thickness measuring units are respectively and symmetrically arranged on the cross beam and the detection frame for detecting the thickness information of the front side and the back side of multi-point positions of the organic glass plate. Because the control system is used for processing and calculating the position information of entity space three-dimensional lattices of the organic glass in real time for confirming the dimension quality of the plates, the on-line double-sided automatic thickness measuring device has the advantage of simple and compact structure, and can be directly arranged at a production line for real-time measurement.