The invention discloses two-dimensional ordered organic semiconductor compound nanometer film and the special-purpose substrate thereof, and the preparation method thereof. Polymer dielectric ultra film provided by the invention is obtained by rubbing or stretching the polymer. The provided two-dimensional ordered organic semiconductor compound nanometer film is formed by compounding the polymer dielectric ultra film and the organic semiconductor compound nanometer film. Through adjusting and controlling the orientation degree, the thickness, the heating temperature, the solution spin coating speed and the vacuum evaporation coating speed of the pre orientated polymer dielectric ultra film, the two-dimensional ordered organic semiconductor compound nanometer film which can meet the ordered structure and the film thickness of different demands is prepared. The preparation method is simple and feasible, the efficiency is high, the cost is low, the performance of the thin film can be greatly improved, the stability is high, and the two-dimensional ordered organic semiconductor compound nanometer film can be used for the fields such as sensing material, electrical conduction material and electro-optical transformation material, etc.