The invention relates to a quantitative constant-temperature agar pouring device. The quantitative constant-temperature agar pouring device is mainly applied to a biological or medical experiment. The quantitative constant-temperature agar pouring device comprises a liquid discharging switch, a quantifying valve, a plunger, a one-way valve, a sample feeding port, a bottle cover, an insulating layer, a storage bottle, a plunger cavity, a return spring, an inlet pipe, a fixing groove, a partition board, electric heating plates, a charging interface, a storage battery, a storage battery switch, a control circuit, a temperature sensor, a temperature display, a control cabinet, a sample feeding table, a constant-temperature water bath, a vent hole and an outlet pipe. The storage bottle with agar is put into the small charged constant-temperature water bath for thermal insulation, and during operation, the quantitative constant-temperature agar pouring device can be placed on a sterile working platform, and through adjustment of the quantifying valve below the liquid discharging switch, the agar pouring volume is controlled to quantitatively pour the constant-temperature agar so as to meet requirements of a pouring culture method, bacterium-containing agar plate preparation and other experiments on sterile operation as well as precise control over the agar pouring volume and the temperature. The quantitative constant-temperature agar pouring device is convenient to move, easy and safe to operate and low in cost, and has relatively high popularization and application values and very strong creativity.