81 results about "Keyhole limpet hemocyanin" patented technology

The present invention provides a method for altering a B cell mediated pathology in a patient. This method comprises administering a composition comprising at least one and / or two chimeric proteins. Each chimeric protein comprises at least a portion of either the VH or VL region of a immunoglobulin molecule from particular B cells from a patient having a B cell mediated pathology, and an immunoglobulin constant region. The genes encoding VH and / or VL regions and the genes encoding immunoglobulin constant regions are isolated and inserted into an expression vector. The chimeric proteins are produced by introducing the expression vectors into insect cell lines. The chimeric proteins are purified using antibody affinity columns, and then chemically conjugated to an immunogenic carrier, keyhole-limpet hemocyanin (KLH). Since the conjugates comprise chimeric proteins made specifically from particular B cells from a patient having B cell mediated pathology, when it is administered to such a patient, with or without a cytokine, such as granulocyte-macrophage-CSF, or a chemokine, it can induce immune responses to alter such a B cell mediated pathology.

A method for preparing multicloning antibody includes synthesizing polypeptide, connecting the coupling agent with keyhole limpet hemocyanin for forming hapten ¿C carrier protein system, immunizing rabbit then picking up its blood for separating out serum, purifying out multicloning antibody of anti ¿C specific site amino acid phosphorylation protein and anti ¿C corresponding non phosphorylation protein after two steps of antigen affinity chromatography .

The invention discloses a method of preparing a bombyx mori silk fibroin specific antibody by utilizing a characteristic dodecapeptide. The method comprises the following steps: synthesizing a polypeptide with a "CGYGAGAGAGYGA" sequence, coupling the polypeptide with keyhole limpet hemocyanin (KLH) so as to obtain a complete antigen; diluting the complete antigen with normal saline, mixing the diluted complete antigen with a complete Freund's adjuvant, carrying out an emulsion treatment so as to obtain primary immunized antigen emulsion, subjecting a rabbit to a primary immunization by using the primary immunized antigen emulsion, then subjecting the rabbit to a strengthened immunization, wherein the strengthened immunization uses a strengthened immunized antigen emulsion, which is prepared by the following steps: mixing the diluted complete antigen with an incomplete Freund's adjuvant, and then carrying out an emulsion treatment so as to obtain the target product; collecting the blood of the immunized rabbit, when the antiserum titer of rabbit arrives at 1 / 10000; making the blood blocks fully contract to completely separate out the antiserum, then collecting the antiserum, and subjecting the antiserum to a centrifugation treatment so as to obtain a supernate. The antibody prepared by the invention has a strong specificity, and can be used for detection and analysis of silk fibroin in textile, and the like.

The invention relates to a complete antigen of AG15 polypeptide. The complete antigen of AG15 polypeptide is formed by the coupling of AG15 polypeptide and keyhole limpet hemocyanin activated by a crosslinking agent, wherein the sequence of AG15 polypeptide is the sequence as shown in SEQ ID No.1. The complete antigen of such AG15 polypeptide is relatively high in immunogenicity and can be put into use as the complete antigen. The invention also discloses a preparation method of the complete antigen of AG15 polypeptide, and an antibody of the complete antigen of AG15 polypeptide. The antibody is specially bound with the complete antigen of AG15 polypeptide. The antibody of the complete antigen of AG15 polypeptide can be used for detecting mice osteocalcin protein in serum or tissue.

IgG and IgM autoantibody levels against phosphorylcholine in subjects with hypertension (diastolic pressure >95 mmHg) were determined at baseline in order to determine the importance of antibodies for the development of atherosclerosis. The results show that increases in intima-media thickness (IMT) at a follow-up four years after baseline were significantly less prevalent in subjects having high autoantibodies particularly high IgM autoantibodies, to phosphorylcholine. The presence or absence of autoantibodies, particularly IgM autoantibodies, against phosphorylcholine is thus related to an increased or decreased risk of developing ischemic cardiovascular diseases. A method to determining antibodies, particularly IgM antibodies, toward phosphorylcholine is proposed in this invention to identify subjects at risk of developing ischemic cardiovascular diseases. Animal experiments show that medium to high levels of antibodies, particularly IgM antibodies, can be detected in plasma after active immunization with a keyhole limpet hemocyanin (KLH-phosphorylcholine conjugate. A pharmaceutical composition comprising a phosphorylcholine conjugate (active immunization) or an antibody preparation, for example a monoclonal antibody, with specificity to a phosphorylcholine conjugate (passive immunization) is proposed and the use of these compositions as active or passive immunogens is the treatment or prevention of atherosclerosis.

The invention discloses a coupling product of streptomycin and carrier protein, and a preparation method of a streptomycin antibody and an application thereof, which relate to a coupling method of the carrier protein such as keyhole limpet hemocyanin, human serum albumin, cow serum albumin, ovalbumin and the like with streptomycin, and immune BALB / C mouse spleen cells are fused with SP2 / 0 mouse myeloma cells by streptomycin immunogens coupled with the carrier protein, and the streptomycin coupled with the carrier protein is used as a coating antigen for screening positive hybridoma and hybridoma that can steady passage and excrete anti-specificity streptomycin monoclonal antibody can be obtained by the cell clone, and ascites monoclonal antibody is prepared. The prepared monoclonal antibody is used for building a direct competition ELISA method with high specificity, sensitivity and accuracy to the streptomycin and immunity colloidal gold test strips. The coupling of the streptomycin and the carrier protein and the preparation method of the streptomycin monoclonal antibody can provide services for detecting streptomycin residue in foods quickly.

Modified Thomsen-Friedenreich disaccharide (TFD) immunogen, preparation procedure, compositions containing it, uses, and treatment methods. More specifically, the present invention refers to a immunogen obtained by modifying TFD, and comprising the general formula D-TFD-Lys(n)-C, wherein D is a hydrophobic terminal residue, TFD is disaccharide Galβ3GalNAcα; Lys(n) is a lysine connector, and n is an integer between 1 and 5, and C is a carrier. In a preferred embodiment, the modified invention immunogen comprises the general formula BzlαTFD-Lys5-KLHs, wherein Bzl is benzyl, TFD is disaccharide Galβ3GalNAcα; Lys5 is penta-lysine, and KLHs is succinilated Keyhole limpets hemocyanin.

The invention relates to a novel method for preparing gonyatoxin GTX2 and GTX3 artificial antigens. The novel method comprises the following steps: introducing two free amino groups in second imido position and the eighth imido position of hapten molecules GTX2 and GTX3 with glyoxal; respectively coupling an intermediate product (H2N-GTX2,3) with vector proteins BSA (Bovine Serum Albumin) and KLH (Keyhole Limpet Hemocyanin) by adopting a two-step method; and respectively carrying out ultrafiltration and purification on the intermediate product and a coupling product and carrying out full-wave band ultraviolet scanning and HPLC (High Performance Liquid Chromatography) appraisal. The appraisal result shows that gonyaulax-paralytic shellfish poisoning GTX2 and GTX3 artificial antigens are successfully prepared according to the research and also have stronger immunogenicity. According to the novel method, the gonyatoxin GTX2 and GTX3 artificial antigens can be effectively prepared, and thus antibodies of the gonyatoxin GTX2 and GTX3 are produced and have important actual significance for quickly detecting the gonyatoxin GTX2 and GTX3 by adopting an immunoassay technology. The whole preparation process is simple, convenient and feasible without adopting special instrument and equipment; and the cost is low and easiness in scale production, popularization and application is realized. The gonyatoxin GTX2 and GTX3 artificial antigens synthesized by the method have better stability.