The invention discloses an antineoplastic medicine prepared through gelsmium elegans medicinal materials. The antineoplastic medicine comprises the following steps that fresh gelsmium elegans rattan stems and/or roots and/or leave and/or dry medicinal slices are taken, steeping and extracting are conducted, ethyl alcohol is recycled by reducing pressure, concentration is conducted until the alcohol taste is eliminated, acid is added for dissolving, PH is adjusted, and upper clear liquid is taken; alkali is added, PH is adjusted to be 9-10, solution extraction is conducted, organic solvent is recycled, and gelsmium elegans total alkaloid extractum is obtained; the extractum is dissolved through methyl alcohol and the like and blended with silica gel, drying and crushing are conducted, the upper silica gel is prepared to a column, gradient elute is conducted by organic solvent, and rough-wrought portions of sempervirine are obtained; and high-middle-pressure phase reversal silica gel column preparation is conducted. The gelsmium elegans total alkaloid begins to exert influences on activity of tumour cells after being added for four hours, and the multiplication speed of the tumour cells is lowered a little compared with a blank group; and the sempervirine begins to exert influences on activity of the tumour cells after being added for five hours, the sempervirine with a low dosage can lower the multiplication speed of the tumour cells, and the sempervirine with a high dosage can kill the tumour cells.