138 results about "Stomach fluid" patented technology

The invention provides an urban sludge treating method, which comprises the following steps of: heating concentrated sludge to 45-55 DEG C, feeding into a high-temperature hydrolytic acidification reactor, and undergoing a high-temperature hydrolysis anaerobic digestion reaction at the pH 6.5-7.5 and at the temperature 40-55 DEG C for 2-5 days to obtain a hydrolytic acidification product; lowering the temperature of the hydrolytic acidification product to 35-40 DEG C, feeding into a medium-temperature methane producing digestion reactor, and undergoing a medium-temperature hydrolysis anaerobic digestion reaction at the temperature 35-38 DEG C for 10-17 days to obtain marsh gas and digested sludge; desulfurizing the marsh gas; and dehydrating the digested sludge to produce dehydrated sludge and digestive fluid. According to the method, resource, minimization and harmlessness of urban sludge treatment are fully realized. The invention further discloses urban sludge treating equipment, which comprises the medium-temperature methane producing digestion reactor, a dehydrator, a desulfurizing tower, a high-temperature hydrolytic acidification reactor and a heat exchanger, wherein a heater is arranged on the high-temperature hydrolytic acidification reactor. The equipment has a simple overall structure, and is easy to realize.

The anti-obesity flow controller includes a tubular structure which is sized to fit within a duodenum in substantially coaxial relation therewith. The anti-obesity flow controller includes a transport structure which is connected to the tubular structure. The tubular structure is secured within the duodenum such that the transport structure provides a conduit for the digestive fluid to flow from the papilla of Vater to the distal end of the tubular structure. The tubular structure is further secured within the duodenum such that the proximal end of the tubular structure communicates with the pylorus to provide for the chyme therefrom to flow into the lumen of the tubular structure. A controller structure is connected to the inner surface of the tubular structure to change the velocity of the chyme which flows within the lumen from the proximal to distal ends.

A bioavailable water soluble animal feed mineral composition which provides buffering of animal digestive fluids to near-neutral pH comprising an alkali metal magnesium phosphate hydrate providing a total of between 1.5 and 4.7 equivalent weights of alkali metal and magnesium per molecule of phosphorus. Water of hydration amounting to between 6 and 12 percent is required for effectiveness. An effective composition, Na2Mg(PO4) 0.8 H2O, is prepared by stepwise reaction of sodium carbonate and magnesium oxide with orthophosphoric acid in a rotating pan equipped for high intensity mixing.

The present invention relates to a controlled dosage release element adapted to be inserted into and retained in the rumen of a ruminant animal. The element comprises: a) one or more discrete and predetermined amounts of at least a first formulation comprising at least a first active agent, the formulation being adapted to dissolve in rumen fluids at a rate such that dissolution of each of the one or more amounts of first formulation provides a short or pulsed episode of release of the first active agent into the rumen; and b) one or more predetermined amounts of at least a second formulation adapted to dissolve at a controlled rate in rumen fluids; wherein the one or more amounts of first formulation are provided at one or more predetermined locations within the element relative to said one or more amounts of second formulation for one or more delayed releases of at least the first active agent into the rumen at predetermined times before, during, after, or any combination thereof, of a predetermined extended time period defined by said second formulation. The invention also relates to a method for delivering at least a first active agent to the rumen of a ruminant animal in a delayed manner at one or more predetermined times after administration to the animal of a composition containing the active agent, the method comprising administering to the animal a controlled dosage release element according to the invention. The first active agent will typically be for the treatment, prophylaxis or both of a diseased or infested state in a ruminant animal, or for altering the physiological status of a ruminant animal.

The invention relates to a preparing method and application of an amniotic membrane, in particular to a preparing method and application of an acellular amniotic membrane used for repairing skin wound difficult to heal. The preparing method specifically comprises the steps of isolating, cleaning and sterilizing an amniotic membrane tissue; arranging and drying the amniotic membrane tissue on a nitrocellulose membrane to manufacture an amniotic membrane paster; vibrating and digesting the amniotic membrane paster in mixed digestive fluid of 0.25%-0.5% of pancreatin and 0.2-0.5g/L EDTA.4Na for 10-30 minutes at a temperature of 37 DEG C; rinsing the amniotic membrane paster in TE buffer solution; vibrating overnight to fully remove amniotic membrane cells in TE-TritonX-100 solution; washing the amniotic membrane paster for three times in TE solution; vibrating and degrading the amniotic membrane paster for 2-4 hours in nucleic acid degrading solution at a temperature of 37 DEG C; washing the amniotic membrane paster for three times in the TE solution. The prepared acellular amniotic membrane paster can be stored for a long time after being refrigerated, dried, packed and sterilized by cobalt 60 irradiation and is a tissue engineering material which can be taken and used when needed. The acellular amniotic membrane paster can be easily separated from the nitrocellulose membrane to be used for repairing the skin wound after rewatered.

The invention belongs to the technical field of wastewater treatment, particularly relates to a treatment method of wastewater anaerobic digestive fluid in a pig farm. The invention uses a water hyacinth pond for removing most of the ammonia nitrogen of wastewater anaerobic digestive fluid in a pig farm, later adopts large-scale emergent aquatic plant pond of water bamboo, reeds, and the like, combines artificial float grass and functional microbial inoculum for purifying the yielding water again so as to lead the concentration of ammonia nitrogen in the yielding water to be further reduced, and then adopts an aquatic animal breeding pond with landscape value for purification treatment. The invention not only can achieve standard discharge of the intensive pig farm wastewater anaerobic digestive fluid, but also can create economic benefits by the aquatic economic plants and aquatic animals in the pond and achieve water resource.

The present invention relates to an apparatus for treating organic waste including: a first circulation line having a first circulation pump connected thereon so as to supply a portion of the organic waste being acid fermented in an acid fermenter to a methane fermenter; a second circulation line having a second circulation pump connected thereon so as to supply a portion of anaerobic digestive fluid methane fermented in the methane fermenter to the acid fermenter; and vortex generating means having a plurality of first, second and third nozzles disposed in the methane fermenter so as to allow the anaerobic digestive fluid in the methane fermenter to be agitated by vortices generated thereof.

The invention discloses a device and a method for realizing sludge digestion fluid short-cut nitrification of continuous flow suspending media. The device is provided with a raw water tank, a denitrification filtering column, a suspending media reactor, a secondary basin and a water outlet tank, wherein the raw water tank is an open casing, the denitrification filtering column is a cylindrical tube, cubic suspending sponge filling materials are filled in the denitrification filtering column, the suspending media reactor is an open basin body with inner cavity divided into a plurality of grid chambers, cubic suspending sponge filling materials are filled in the grid chambers, further the suspending media reactor is equipped with a gas pump, a gas flow meter and an aerated conduit, the secondary basin is a column, and the water outlet tank is a open casing. The method for realizing sludge digestion fluid short-cut nitrification comprises starting the system and adjusting operation in running. The device and the method can save a large amount of denitrification carbon sources, increase denitrogenation efficiency of sewage disposal plants, and achieve the purpose of saving energy and lowering energy consumption.

The invention discloses a hair pretreatment method belonging to the technical field of biological chemistry. The method comprises the following steps: cleaning hair by using a cleaning agent, and airing; crushing hairs into fragments, and finally, adding the hair fragments into digestive fluid, putting the digestive fluid into a water bath at the temperature of 60-100 DEG C for 5 minutes to 2 hours so as to hydrolyze the hairs, standing and cooling the digestive fluid to room temperature after the hydrolysis is finished, and detecting supernatant. According to the method disclosed by the invention, the hairs serve as a detection material, are easy to sample, non-traumatic and convenient to preserve and can be repeatedly sampled, the steps of a conventional hair pretreatment method are simplified and optimized, various components in the used digestive fluid do not influence with one another, the detection effect is good, the detection accuracy is high, the false positive condition is difficultly caused, and the misjudgment condition is avoided as much as possible in the detection process.

The invention relates to an erythroculter ilishaeformis spermatogonia stem cell separation and culture method. The method comprises the following steps: (1) collecting spermatic tissues: collecting spermary of erythroculter ilishaeformis with an age of seven months in a sterile environment, then rinsing spermary in PBS containing double-antibody, and finally grinding the spermary to disperse the spermary tissues; (2) digesting and separating spermatogonia stem cell: adding IV type collagenase (0.1%) into the dispersed spermary tissues, subjecting the digestive fluid to centrifugal separation, collecting the precipitate, digesting the precipitate by trypsin (0.25%), stopping the digestion by a culture medium containing FBS (10%), and collecting the cells; (3) carrying out primary culture of spermatogonia stem cell: using a DMEM/F12 complete medium containing cell factors to re-suspend the cells obtained in the step (2), adjusting the cell concentration, then paving the suspension liquid on a 24-hole cell culture plate coated by gelatin, and culturing the cells at a temperature of 26 DEG C. Trough the provided method, the in-vitro growth of primary cells of erythroculter ilishaeformis spermatogonia stem cell becomes easier, and an effective cell platform is provided for the research on reproduction and growth of erythroculter ilishaeformis.

The invention relates to a three-dimensional culture system based on thermo-sensitive biogel. The three-dimensional culture system is prepared by combining the following reagents: thermo-sensitive biogel, digestive fluid required for separating and culturing tumor cells, a basal culture medium and a human intestinal stem cell culture medium. The invention also provides a method for establishing acolorectal cancer patient-derived xenograft model through the three-dimensional culture system based on thermo-sensitive biogel. The three-dimensional culture system has the advantages that colorectalcancer cells can be further amplified and grown in the three-dimensional culture system based on thermo-sensitive biogel, and the tumor formation capability of the colorectal cancer cells in the immune deficient mice can be enhanced. Polyclonal tumor cells cultured in the system have high subcutaneous tumor formation capability in nude mice. The three-dimensional culture system has the advantagesof simple preparation method and mature technology, and is capable of greatly reducing the time and the cost for establishing the human-derived colorectal cancer xenograft model.

A simple in vitro liver perfusion apparatus for digesting and separating liver cell characterized in that a container is installed on the engine block, inside the container being positioned a stainless reticular baffle plate, a heating device being installed in the container bottom under the stainless reticular baffle plate, a perfusion bottle with a side hole is fixed on the top surface of stainless reticular baffle plate by a fixing clamp, and a stainless filter net is fixed on the top part by bracket. An input end of peristaltic pump on the engine block is connected with an aseptic input conduct, while the out put end of the peristaltic pump is connected with an aseptic outputting conduct, needle handle and perfusion needle. It can realize two step liver perfusion for in vitro liver, obtain effective perfusion for anterior perfusion fluid and digestive fluid rapidly and continuously in the largest extent, and monitor liver tissue process

The invention discloses an Bifidobacterium animal subspecies lactis JMCC0025. The strain of JMCC0025 was deposited in General Microbiology Center of China Microbiological Culture Collection ManagementCommittee on August 20, 2019, the deposit address is No. 3, yard 1, Beichen West Road, Chaoyang District, Beijing, and the deposit number is CGMCC NO. 18403; and the invention also discloses a methodof isolation and purification and application of the above Bifidobacterium animal subspecies lactis JMCC0025. The Bifidobacterium animal subspecies lactis JMCC0025 provided by the present invention can regulate intestinal flora balance, improve fecal characteristics, and has better better survival rate of simulated digestive fluid; and under the storage condition of 37 DEG C, which is suitable for the growth of lactic acid bacteria, the post-acid can be well controlled. The invention belongs to the technical field of bioengineering, and the provided Bifidobacterium animal subspecies lactis JMCC0025 has the function of regulating intestinal flora balance, and can be used in the process of making food, medicine or beverage.

The invention discloses a tower-type self-circulation aerobic degradation reactor. Waste water is uniformly introduced into an anoxic reaction zone at the bottom of the reactor by virtue of a water inlet pipe and a water distribution system which are arranged at the bottom of the reactor, the waste water is jointly mixed and reacts with high-concentration sludge and digestive fluid which are arranged at the bottom of the reactor and mixed liquor refluxed through a central reflux pipe, the mixture rises for forming a vertically rising type flow state upward movement, and a water inlet pump and an aeration fan are used for providing power, so that no sludge, digestive fluid reflux pump and the like is needed; meanwhile, the aeration fan has low power requirement, a conventional air blower is needed only, and operating cost and management difficulty are greatly reduced.

The invention discloses a harmless wastewater treatment system for animal carcasses and a wastewater treatment method. The harmless wastewater treatment system comprises a wastewater pretreatment unit, a hydrolytic acidification reactor and an MBBR (moving bed bioreactor)-MBR (membrane bioreactor) combined biological treatment unit, wherein the wastewater pretreatment unit comprises a catalytic ozonation reactor, an activated sludge adsorptive reactor and an ammonia-nitrogen air stripping absorption tower; the MBBR-MBR combined biological treatment unit comprises an anoxic MBBR, aerobic MBBRs and an MBR, aeration devices are arranged in the aerobic MBBRs and the MBR respectively, and a digestive fluid reflux pipeline is arranged between the MBR and the anoxic MBBR. The wastewater treatment method comprises steps of pretreatment, hydrolytic acidification and biological treatment. With adoption of the system and the method, wastewater produced during harmless treatment of the animal carcasses can be sterilized and disinfected, the system and the method adapt to characteristics of the wastewater produced during harmless treatment of the animal carcasses, and the ammonia-nitrogen removal rate is high.

The invention discloses a device and a method for preparing a biological gas and a liquid organic fertilizer from fruit and vegetable wastes of a collecting and distributing center. The method comprises the following steps: squeezing and crushing the fruit and vegetable wastes collected by a collecting and distributing center to obtain a crushed solid raw material and a liquid, and directly feeding the liquid into an anaerobic fermentation reactor for fermentation; feeding the crushed solid raw material into a rotatable hollowed-out cylindrical hydrolyzing device arranged in a hydrolyzing and desilting basin for hydrolyzing and desilting, thereby obtaining a hydrolysate; and then feeding the hydrolysate into the anaerobic fermentation reactor for fermentation to generate methane, and sterilizing the digestive fluid left after fermentation at a high temperature to obtain a liquid fertilizer. According to the method, the fermentation period is shortened, the fermentation efficiency of the raw material is improved, the problems of difficult sorting of small impurities, huge influence on the anaerobic fermentation process, difficult degradation of high-fiber content wastes and the like are overcome, the impurities are prevented from entering the anaerobic fermentation reactor, and the fruit and vegetable waste processing capability and the gas producing capability are improved; meanwhile, the high-quality liquid organic fertilizer is produced, the cost is reduced and the efficiency is improved.

The invention belongs to the technical field of environmental protection, and particularly relates to a method for treating waste liquid of sweet potato fuel alcohol. The process flow of the method is as follows: sequential batch ASB (Anaerobic Sludge Bed) reaction -> two-stage SBBR (Sequential Batch Biofilm Reactor) aerobic reaction -> flocculation precipitation. In the anaerobic reaction, intermittent sequential batch water feeding is adopted, and after digestive fluid is settled on standing, water is discharged; in the aerobic reaction, a denitrifier agent is added; and in the flocculation precipitation, a high-efficiency composite flocculating agent formed by mixing polyferric sulfate and ferric trichloride heptahydrate is added. The method has the advantages of high anaerobic organic load, short hydraulic retention time, high organic matter removing rate and high methane yield, and the difficult problem that a high-efficiency anaerobic reactor is hard to run due to high inflow water SS (Suspended Solid) concentration; the removing rate of aerobic organic matters is high and the denitrification effect is good; and in flocculation precipitation, the removal rate to chromaticity and TP (Total Phosphorus) is more than 95%, the removal rate of COD (Chemical Oxygen Demand) is about 70%, and the finally discharged water reaches the primary standard of integrated sewage discharge standard GB8978-1996.