21 results about "Stomach fluid" patented technology

The invention relates to the technical field of livestock breeding improvement, and discloses a beef cattle high intramuscular fat content prediction method and application. The beef cattle high intramuscular fat content prediction method comprises the following steps: step 1, obtaining a rumen fluid sample of beef cattle to be detected; step 2, detecting the contents of myristic oleic acid, citric acid and salicylate in the rumen fluid sample; and step 3, comparing the content with a preset threshold value, and if the content of at least one metabolite is higher than the corresponding preset threshold value, predicting that the beef cattle has high intramuscular fat deposition potential or high intramuscular fat content. According to the method, early-stage, rapid and accurate prediction of the high intramuscular fat deposition potential of the individual cattle can be realized, the limitation that the traditional method depends on post-slaughter phenotype determination or is long in gene breeding period and high in cost is overcome, and a brand new technical means is provided for early-stage seed selection and precise breeding.

The present invention provides a self-assembling peptide gel for use in preventing a leak of digestive fluid and a self-assembling peptide gel for use in protecting an organ against digestion by a digestive fluid. The self-assembling peptide gel comprises a self-assembling peptide and water, whereby the self-assembling peptide gel has an adhesion persistence ratio with respect to a collagen sheet of at least 40% and a decomposition ratio of 35% or less in pancreatic juice treatment at 37°C for 7 days, and the self-assembling peptide gel is positively charged as a whole.

The present invention provides a material or method that can serve as an alternative to a related-art digestive fluid leak preventative material and method of preventing a leak of a digestive fluid. The digestive fluid leak preventative material of the present invention includes a self-assembling peptide gel, has an adhesion persistence ratio with respect to a collagen sheet of at least 40%, and has a decomposition ratio of 35% or less in pancreatic juice treatment at 37°C for 7 days.

This invention belongs to the field of food nutrition and functional factors, specifically relating to a method for preparing highly immunomodulatory ovotransferrin (OVT) hydrolysates based on an adult gastrointestinal simulated digestive system. The method involves processing OVT using a simulated adult gastrointestinal tract to prepare OVT hydrolysates containing HTEGSTT, TTSY, and / or RTAGWVIPMG. This invention reveals that OVT exhibits enhanced immunomodulatory activity after gastrointestinal digestion and hydrolysis, and is more readily hydrolyzed in simulated adult digestive fluids than in infant digestive fluids. Comparative experiments show that nutritional intervention with OVT simulated digestive products from adults and infants can promote the production of short-chain fatty acids such as acetic acid, propionic acid, and butyric acid, effectively alleviate swelling and damage to the small intestinal mucosa caused by inflammation, significantly improve intestinal barrier immune function, and OVT IA exhibits higher intestinal immune activity than OVT IB.

The invention belongs to stomach tubes, and particularly relates to a neonatal tube feeding and drainage integrated device which comprises a middle tube body composed of a first channel and a second channel which are independent from each other. The external joint comprises a first joint and a second joint; the far-end connector covers the ends of the first channel and the second channel, one end of the first channel is communicated with the interior of the far-end connector, the other end of the first channel is communicated with the first connector, one end of the second channel is communicated with the interior of the far-end connector, and the other end of the second channel is communicated with the second connector. The first channel and the second channel on one side of the external joint are not communicated with each other so as to avoid flow path interference; the far-end connector and the channel are provided with a first through hole and a second through hole which are used for being communicated with a gastral cavity. The state switching sealing assembly is arranged, so that the device closes the second channel in the first state so as to achieve stomach gas discharge during feeding, the first channel and the second channel are communicated in the second state so as to achieve gastric juice suction, the risks of abdominal distension, backflow and aspiration are reduced, and state switching is simple, rapid and effortless.

The invention provides a bionic stomach micro-fluidic chip based on liquid drop micro-fluidic control and mechanical force stimulation and application of the bionic stomach micro-fluidic chip. The bionic stomach micro-fluidic chip comprises a liquid drop generation module and a mechanical force stimulation module. The liquid drop generation module is used for dispersing artificial stomach digestive juice and a to-be-digested Newtonian fluid sample in an oil phase by utilizing a micro-fluidic chip structure to form monodispersed water-in-oil liquid drops to simulate a discrete chyme group; and the mechanical force stimulation module is provided with a micro-channel with a periodically variable cross section. When liquid drops flow through the flow channel, the liquid drops are extruded and deformed periodically, a complex flow field is generated in the liquid drops, and the mechanical kneading effect of stomach wall wriggling on chyme balls is simulated. By limiting biochemical reaction in micro-droplets and applying controllable physical shear force, collaborative high-fidelity simulation of chemical and mechanical effects in the gastric digestion process is realized, and the problems that a traditional static model is low in simulation degree and large dynamic equipment is low in flux and high in cost are solved. And a brand new high-throughput research platform is provided for the fields of food and medicine.

The invention discloses application of methyl nicotinate as a marker in evaluation of bull semen quality, and belongs to the technical field of animal reproduction and metabonomics. The relative content of methyl nicotinate in the bull rumen fluid with high semen quality is significantly higher than the relative content of methyl nicotinate in the bull rumen fluid with low semen quality. By detecting the relative content, the bull semen quality is evaluated, the reproductive performance can be reliably judged on the premise of not depending on a semen sample, and the method has the remarkable advantages of being non-invasive, high in throughput, capable of advancing a prediction window and the like.

This invention discloses the application of a nutritional composition in the preparation of immunomodulatory products. The nutritional composition comprises lactoferrin gastrointestinal digests and selenium gastrointestinal digests; wherein the content of lactoferrin gastrointestinal digests in the nutritional composition is 80-180 μg / mL; and the content of selenium gastrointestinal digests, calculated as selenium, is 12-28 ng / mL. The nutritional composition provided by this invention, comprising a digestive fluid of lactoferrin and selenium, exhibits a better synergistic anti-inflammatory effect compared to lactoferrin or selenium alone.

To provide a material or a method capable of replacing a conventional digestive fluid leakage preventing material and a conventional digestive fluid leakage preventing method.SOLUTION: The digestive fluid leakage prevention material of the present invention contains a self-assembling peptide gel, has an adhesion residual ratio of at least 40% with respect to a collagen sheet, and has a degradation rate of 35% or less when treated with pancreatic fluid at 37 °C for 7 days.SELECTED DRAWING: None

This invention relates to a method for determining the release rate at various points of a rumen-exposed product in ruminants, comprising the following steps: water solubility determination: weighing an appropriate amount of rumen-exposed sample and determining its water solubility; acid solubility determination: weighing an appropriate amount of rumen-exposed sample and determining its acid solubility; rumen degradation rate determination: weighing the rumen-exposed sample after water and / or acid solubility determinations, placing it on feed, adding buffer solution and rumen fluid for in vitro fermentation, drying at a constant temperature after fermentation, weighing, and determining the rumen degradation rate; abomasal degradation rate determination: weighing the rumen-exposed sample after rumen degradation rate determination, adding pepsin hydrochloride buffer solution for incubation at a constant temperature, removing and drying at a constant temperature after incubation, weighing, and determining the abomasal degradation rate; small intestinal degradation rate determination: taking the rumen-exposed sample after abomasal degradation rate determination, adding small intestinal buffer solution for shaking incubation, washing, drying at a constant temperature, weighing, and determining the small intestinal degradation rate.

The application discloses a camel special rumen internal environment monitoring device, which comprises an insertion collection component, a suction component is fixedly connected to one end of the insertion collection component, and the insertion collection component is used for inserting into a camel rumen to collect rumen fluid; a bite prevention component is fixedly arranged on the insertion collection component; and a sealing adjustment component is arranged. In the prior art, a common rumen internal environment monitoring device is contacted with an esophagus during insertion into a rumen, part of esophageal fluid or food residues enter the monitoring device, and monitoring errors are caused. By arranging the sealing adjustment component, automatic sealing protection can be realized during insertion, the sealing adjustment component can be adjusted to be opened after complete insertion into the rumen, rumen fluid can be collected and detected, and the phenomenon that other foreign matters are introduced due to contact with the esophagus during insertion is effectively avoided.

The invention provides a compound feed additive for ruminant rumen methane emission reduction and a verification method thereof, and relates to the technical field of feed additives and ruminant nutrition regulation and control. The compound feed additive is composed of nitroethane, fumaric acid and 3-nitrooxy propyl alcohol according to the molar ratio of 1: 1: 1, and effective action concentration is formed in rumen fermentation liquor. The method comprises the following steps: constructing a control group and a treatment group from basic ration, artificial saliva and rumen fluid by utilizing an in-vitro simulated rumen fermentation system, carrying out anaerobic culture at 39 DEG C for 24 hours, and then measuring the methane content, fermentation parameters and volatile fatty acid composition so as to evaluate the emission reduction effect. The compound feed additive disclosed by the invention can cooperatively regulate and control rumen hydrogen metabolism, keeps rumen fermentation stability while remarkably inhibiting methane generation, and provides a feasible technical approach for low-methane-emission feeding of ruminants.

The invention discloses a device for capturing sheep and conveniently collecting rumen fluid, and relates to the technical field of equipment for animal experiments, and the key point of the technical scheme is that the device comprises a disc, an automatic coiling assembly is arranged in the disc, the tail end of the automatic coiling assembly is connected with one side of a T-shaped mouth gag, and the other side of the T-shaped mouth gag is connected with a motor; the other side of the T-shaped mouth gag is connected with one end of a fixing line, the other end of the fixing line is fixedly connected with the disc, a telescopic assembly is arranged at the top of the disc, and a fixing assembly is arranged at the top of the telescopic assembly. The device provided by the invention is simple to operate, does not easily cause stress of the sheep, and is convenient for taking rumen fluid.

This invention provides a magnetothermal phase change microrobot, its preparation method and application, and a targeted anchoring and sealing method, belonging to the field of biomaterials technology. The magnetothermal phase change gel provided by this invention integrates magnetically driven motion and adaptive wound filling functions, offering an effective technical means for non-invasive targeted treatment of enterocutaneous fistulas. Specifically, this invention applies a rotating magnetic field, guiding the magnetothermal phase change gel to the fistula opening and achieving initial filling under the action of a ferromagnetic body and magnetic torque. At this time, an alternating magnetic field is applied, and under the hysteresis effect, the paramagnetic body generates heat, heating the magnetothermal phase change gel to its phase transition temperature, i.e., a transition from solid to liquid, achieving adaptive and precise filling of irregular fistula openings in a fluid state. Subsequently, the alternating magnetic field is removed, and as the temperature decreases, the liquid state returns to solid, thereby tightly sealing the fistula opening and effectively preventing leakage of digestive fluids.

This invention discloses a method for anaerobic co-fermentation of cyanobacterial sludge to produce acid through pretreatment with bovine rumen fluid. 6–12 g of bovine rumen fluid is added to every 100 mL of the cyanobacterial sludge-water mixture, and the mixture is pretreated at 35–39°C for 12–24 h. Without removing or deactivating the bovine rumen fluid, it is mixed with inoculum sludge, and the pH of the system is adjusted to 9.5–10.5. After replacing oxygen with an inert gas, anaerobic fermentation is carried out at 35–37°C, maintaining a stirring speed of 120–150 rpm. This method can promote the dissolution of proteins and carbohydrates in cyanobacterial sludge and increase the yield of acetic acid and volatile fatty acids, making it suitable for the resource utilization of cyanobacterial sludge and the preparation of alternative carbon sources.

The invention discloses a ruminococcus buchneri fermentation growth medium and a preparation method thereof. The growth medium is prepared from the following raw materials: a carbon source, a nitrogen source, inorganic salt, a reducing agent, a redox indicator, cooked beef granules and bovine rumen fluid, wherein the carbon source is resistant starch, and the concentration of the carbon source is 3.0-7.0 g / L; the concentration of the nitrogen source is 16.0-67.0 g / L; the reducing agent is L-cysteine hydrochloride, and the concentration of the reducing agent is 0.1-0.7 g / L; the redox indicator is resazurin, and the concentration of the redox indicator is 0.001-0.007 g / L; the volume ratio of the bovine rumen fluid is 5%-25%; the mass volume ratio content of the cooked beef granules is 10.0-40.0 g / L. By optimizing the formula of the culture medium, the growth efficiency and metabolism of the ruminococcus buchneri are remarkably improved, and the growth activity is prolonged, which is specifically reflected in multiple aspects such as thallus growth density, physiological functions and economy.

The invention provides a pH response type active bacterium raft and a preparation method and application thereof, and belongs to the field of biomedical engineering.The preparation method of the pH response type active bacterium raft comprises the following steps that 1, calcium carbonate, sodium bicarbonate and a sodium alginate solution are mixed and stirred for the first time, and a mixed solution is obtained; (2) dropwise adding lactococcus lactis into the mixed solution obtained in the step (1), and carrying out secondary stirring to obtain a bacterium-sodium alginate mixed system; and 3) injecting the bacteria-sodium alginate mixed system into a cross-linking system through electrostatic spinning to obtain the pH response type active bacteria raft. The pH response type active bacteria raft and the lactococcus lactis are uniformly distributed in a hydrogel structure rich in bubbles, have a remarkable and acid-stable bubble structure, and can stably float in simulated gastric juice to regulate and control pH; the esophageal injury inhibition rate can reach 93.95% in the gastroesophageal reflux disease treatment process, and excellent clinical transformation prospects are shown.

The utility model discloses a drainage device for ascites discharge of tumor patients, which comprises a drainage bag, a connecting piece is arranged at the top of the drainage bag, a detachable drainage tube is arranged in the connecting piece, the drainage tube is communicated with an inner cavity of the drainage bag through the connecting piece, and a communicating tube communicated with the inner cavity of the drainage bag is mounted at the top of the drainage bag. The top end of the communicating pipe penetrates through and extends out of the drainage bag, a flow guide pipe communicated with an inner cavity of the communicating pipe is installed at the top end of the communicating pipe, the bottom end of the communicating pipe is sealed, and a first communicating hole communicated with the inner cavity of the communicating pipe is formed in the upper portion of the side face of the communicating pipe. The drainage device for ascites drainage of the tumor patient can be used for drainage of ascites and collection of ascites, medical staff can conveniently judge whether postoperative bleeding, digestive juice leakage and the like exist in the abdominal cavity or not according to information such as the ascites color and the drainage amount, meanwhile, the drainage device can conveniently sample the collected ascites, and the drainage effect is good. And the ascites characteristics can be conveniently detected by medical staff.

A fully automatic intelligent modular biomimetic human digestive apparatus, and a method. A modular biomimetic complete human digestive system accurately simulates the human digestive process, and comprises key organs such as the oral cavity, the stomach, and the small intestine. Each module has real-time measurement, precise control, and sample collection functions, ensuring that the digestive process is highly controllable and observable. An intelligent control system utilizes sensor technology and microprocessor algorithms to automatically regulate and optimize module operations. The modular structure makes the system easy to assemble and maintain, enables customized configuration as required, and provides flexible, tailored solutions for digestive physiology research. The system comprises modules such as an oral digestion module, a gastric digestion module, a small intestine digestion module, a digesta recovery module, a sample testing module, a power supply module, and a digestive fluid supply module. Each module performs a specific function to collectively ensure the accuracy and controllability of the simulated digestive process.

Provided is a protective adhesive composition for digestive tract mucosa and application thereof, belonging to the technical field of biomedicine. The protective adhesive composition includes an adhesive-forming substance, a cross-linking agent, an adhesive agent, and an acid-base regulator. The protective adhesive composition may form a colloidal membrane, and has acid-resisting and pepsin-resisting effects, such that a stimulating effect of digestive fluids on injured mucosa is avoided, thereby promoting the healing of wounds.