44 results about "Stomach fluid" patented technology

The invention discloses Pediococcus pentosaceus G41, which is separated from rumen fluid of healthy adult dairy cows in a certain cattle farm in Changchun, has the advantages of high growth speed and strong acid production capacity, and can effectively relieve cholestatic liver injury. Experiments prove that the Pediococcus pentosaceus G41 reduces the activity of serum AST, ALT and GDH of a mouse with cholestatic liver injury, reduces the content of total bile acid in blood and liver, increases the content of total bile acid in excrement, and improves cholestatic and dirty injury caused by cholestatic.

The present invention provides a material or method that can replace conventional materials and methods for preventing digestive fluid leakage. The digestive fluid leakage preventing material of the present invention comprises a self-assembling peptide gel, has an adhesion retention rate of at least 40% on a collagen sheet, and a decomposition rate of 35% or less based on pancreatic juice treatment at 37°C for 7 days.

The invention relates to the technical field of livestock breeding improvement, and discloses a beef cattle high intramuscular fat content prediction method and application. The beef cattle high intramuscular fat content prediction method comprises the following steps: step 1, obtaining a rumen fluid sample of beef cattle to be detected; step 2, detecting the contents of myristic oleic acid, citric acid and salicylate in the rumen fluid sample; and step 3, comparing the content with a preset threshold value, and if the content of at least one metabolite is higher than the corresponding preset threshold value, predicting that the beef cattle has high intramuscular fat deposition potential or high intramuscular fat content. According to the method, early-stage, rapid and accurate prediction of the high intramuscular fat deposition potential of the individual cattle can be realized, the limitation that the traditional method depends on post-slaughter phenotype determination or is long in gene breeding period and high in cost is overcome, and a brand new technical means is provided for early-stage seed selection and precise breeding.

The present invention provides a self-assembling peptide gel for use in preventing a leak of digestive fluid and a self-assembling peptide gel for use in protecting an organ against digestion by a digestive fluid. The self-assembling peptide gel comprises a self-assembling peptide and water, whereby the self-assembling peptide gel has an adhesion persistence ratio with respect to a collagen sheet of at least 40% and a decomposition ratio of 35% or less in pancreatic juice treatment at 37°C for 7 days, and the self-assembling peptide gel is positively charged as a whole.

The present invention provides a material or method that can serve as an alternative to a related-art digestive fluid leak preventative material and method of preventing a leak of a digestive fluid. The digestive fluid leak preventative material of the present invention includes a self-assembling peptide gel, has an adhesion persistence ratio with respect to a collagen sheet of at least 40%, and has a decomposition ratio of 35% or less in pancreatic juice treatment at 37°C for 7 days.

The present invention discloses a method of enabling a better and more controlled drug release from an oral administered pill and / or an oral delivery device by using gravity adding weight / high density content to secure that the pill / device decent through the stomach fluids and come in close contact with stomach and / or intestine tissue as it dissolves / releases the drug enabling a better controlled absorption / transfer of the drug to the blood stream.

This invention belongs to the field of food nutrition and functional factors, specifically relating to a method for preparing highly immunomodulatory ovotransferrin (OVT) hydrolysates based on an adult gastrointestinal simulated digestive system. The method involves processing OVT using a simulated adult gastrointestinal tract to prepare OVT hydrolysates containing HTEGSTT, TTSY, and / or RTAGWVIPMG. This invention reveals that OVT exhibits enhanced immunomodulatory activity after gastrointestinal digestion and hydrolysis, and is more readily hydrolyzed in simulated adult digestive fluids than in infant digestive fluids. Comparative experiments show that nutritional intervention with OVT simulated digestive products from adults and infants can promote the production of short-chain fatty acids such as acetic acid, propionic acid, and butyric acid, effectively alleviate swelling and damage to the small intestinal mucosa caused by inflammation, significantly improve intestinal barrier immune function, and OVT IA exhibits higher intestinal immune activity than OVT IB.

The pediatric gastric lavage device comprises a liquid storage tank, a stirring and heating mechanism, an air cylinder, a waste liquid tank, an outer hose, an electric heating layer, an inner hose, a cleaning mechanism, a recycling mechanism and a rubber ball, the liquid storage tank is arranged on the rear side of the upper portion of a bottom plate, the stirring and heating mechanism is arranged in the liquid storage tank, and the waste liquid tank is arranged on the upper portion of a mounting plate; the electric heating layer is fixedly connected to the inner side of the outer hose, the cleaning mechanism is arranged between the outer hose and the liquid storage tank, and the recycling mechanism is arranged between the outer hose and the waste liquid tank. Compared with the prior art, the gastric lavage device has the advantages that normal saline and medicine in the liquid storage box can be fully stirred and mixed through the stirring and heating mechanism, the uniformity and the using effect of gastric lavage liquid are guaranteed, and meanwhile the heating bin is arranged to be matched with the electric heating pipe to heat liquid in the liquid storage box; the flushing is milder, and the stomach of the child cannot be stimulated.

The invention belongs to stomach tubes, and particularly relates to a neonatal tube feeding and drainage integrated device which comprises a middle tube body composed of a first channel and a second channel which are independent from each other. The external joint comprises a first joint and a second joint; the far-end connector covers the ends of the first channel and the second channel, one end of the first channel is communicated with the interior of the far-end connector, the other end of the first channel is communicated with the first connector, one end of the second channel is communicated with the interior of the far-end connector, and the other end of the second channel is communicated with the second connector. The first channel and the second channel on one side of the external joint are not communicated with each other so as to avoid flow path interference; the far-end connector and the channel are provided with a first through hole and a second through hole which are used for being communicated with a gastral cavity. The state switching sealing assembly is arranged, so that the device closes the second channel in the first state so as to achieve stomach gas discharge during feeding, the first channel and the second channel are communicated in the second state so as to achieve gastric juice suction, the risks of abdominal distension, backflow and aspiration are reduced, and state switching is simple, rapid and effortless.

The utility model relates to the technical field of gastrointestinal department medical instruments, in particular to a digestive juice filtering and reinfusion device which comprises a shell, an input pipe and an output pipe, the input pipe and the output pipe are communicated with the interior of the shell, a filter plate and a partition plate are arranged in the shell to divide the interior of the shell into an input cavity, a middle cavity and a reinfusion cavity in sequence, and the input cavity is communicated with the input pipe. The middle cavity is communicated with the output pipe, the middle cavity is externally connected with a negative pressure device, the partition plate is provided with a valve, the valve has an opening state and a closing state, when negative pressure is formed in the middle cavity, the valve maintains the closing state, and when hydraulic pressure in the middle cavity is larger than acting force needed by closing of the valve, the valve is opened so that the middle cavity and the feedback cavity can be communicated. The problems that an existing digestive juice filtering and returning device is relatively complex in structure and relatively troublesome to use are solved.

The invention relates to a method for measuring the release rate of each site of a ruminant rumen bypass product, which comprises the following steps: measuring the water solubility: weighing a proper amount of rumen bypass sample, and measuring the water solubility; measuring the acid solubility: weighing a proper amount of rumen bypass sample, and measuring the acid solubility; rumen degradation rate determination: weighing a rumen bypass sample after water solubility determination and / or acid solubility determination, putting the rumen bypass sample on a feed, adding a buffer solution and rumen fluid for in-vitro fermentation, after fermentation is finished, performing constant-temperature drying, then performing weighing, and performing rumen degradation rate determination; measuring the abomasum degradation rate: weighing the rumen bypass sample after the rumen degradation rate measurement, adding a hydrochloric acid buffer solution of pepsin for constant-temperature culture, taking out the rumen bypass sample after the culture is finished, drying at constant temperature, weighing, and measuring the abomasum degradation rate; and small intestine degradation rate determination: taking the rumen bypass sample after abomasum degradation rate determination, adding a small intestine buffer solution, carrying out shake culture, cleaning, drying at a constant temperature, weighing, and determining the small intestine degradation rate.

The present invention discloses a method and application for encapsulating tributyrin with a hydrophilic barrier layer to aid probiotics in encapsulating tributyrin, belonging to the field of oil microencapsulation technology. The method comprises the following steps: 1) mixing probiotic cells with tributyrin and washing with an aqueous solution to obtain a first complex containing probiotics encapsulating tributyrin; and 2) mixing the first complex with a hydrophilic solution to obtain a second complex containing probiotics encapsulating tributyrin with a hydrophilic barrier layer. The second complex (tributyrin-encapsulated complex) prepared using the present method exhibits high loading capacity, lacks surface oil, and completely masks the odor of tributyrin. It can release tributyrin in digestive fluids and exert intestinal health benefits. It can be used in food, medicine, and feed, as well as in oral or parenteral formulations.

The application provides an article information pushing method combining article attributes and user preferences, comprising: obtaining digestive system disease types, digestive tract absorption capacity, digestive fluid secretion conditions and physiological characteristic data related to digestive function of a plurality of users, grouping the users through a clustering algorithm to obtain user groups with different digestive function characteristics; obtaining dosage form, particle size and surface area attribute information of health products, reducing the dimensions of the health products through principal component analysis according to disintegration time, dissolution rate and osmotic pressure characteristics of the dosage form to obtain a key attribute set of health products with different dosage forms; obtaining health product information searched by the user and digestive condition data of the user, and judging whether the user belongs to a group with weak digestive tract absorption capacity or a group with normal digestive function according to a set digestive threshold.

The invention provides a bionic stomach micro-fluidic chip based on liquid drop micro-fluidic control and mechanical force stimulation and application of the bionic stomach micro-fluidic chip. The bionic stomach micro-fluidic chip comprises a liquid drop generation module and a mechanical force stimulation module. The liquid drop generation module is used for dispersing artificial stomach digestive juice and a to-be-digested Newtonian fluid sample in an oil phase by utilizing a micro-fluidic chip structure to form monodispersed water-in-oil liquid drops to simulate a discrete chyme group; and the mechanical force stimulation module is provided with a micro-channel with a periodically variable cross section. When liquid drops flow through the flow channel, the liquid drops are extruded and deformed periodically, a complex flow field is generated in the liquid drops, and the mechanical kneading effect of stomach wall wriggling on chyme balls is simulated. By limiting biochemical reaction in micro-droplets and applying controllable physical shear force, collaborative high-fidelity simulation of chemical and mechanical effects in the gastric digestion process is realized, and the problems that a traditional static model is low in simulation degree and large dynamic equipment is low in flux and high in cost are solved. And a brand new high-throughput research platform is provided for the fields of food and medicine.

The invention discloses application of methyl nicotinate as a marker in evaluation of bull semen quality, and belongs to the technical field of animal reproduction and metabonomics. The relative content of methyl nicotinate in the bull rumen fluid with high semen quality is significantly higher than the relative content of methyl nicotinate in the bull rumen fluid with low semen quality. By detecting the relative content, the bull semen quality is evaluated, the reproductive performance can be reliably judged on the premise of not depending on a semen sample, and the method has the remarkable advantages of being non-invasive, high in throughput, capable of advancing a prediction window and the like.

The present invention relates to compositions in the form of PET- and lecithin-free microemulsion preconcentrates that contain at least one cannabinoid, at least one polyglyceryl- containing surfactant, at least one polyol and a preservative. When contacted with either an aqueous medium, gastric or intestinal fluid, the microemulsion preconcentrates self-assemble into microemulsions that are stable in gastric and intestinal fluids. The invention is also related to methods of making and methods of using the microemulsion preconcentrates.

This invention discloses the application of a nutritional composition in the preparation of immunomodulatory products. The nutritional composition comprises lactoferrin gastrointestinal digests and selenium gastrointestinal digests; wherein the content of lactoferrin gastrointestinal digests in the nutritional composition is 80-180 μg / mL; and the content of selenium gastrointestinal digests, calculated as selenium, is 12-28 ng / mL. The nutritional composition provided by this invention, comprising a digestive fluid of lactoferrin and selenium, exhibits a better synergistic anti-inflammatory effect compared to lactoferrin or selenium alone.

To provide a material or a method capable of replacing a conventional digestive fluid leakage preventing material and a conventional digestive fluid leakage preventing method.SOLUTION: The digestive fluid leakage prevention material of the present invention contains a self-assembling peptide gel, has an adhesion residual ratio of at least 40% with respect to a collagen sheet, and has a degradation rate of 35% or less when treated with pancreatic fluid at 37 °C for 7 days.SELECTED DRAWING: None

This invention relates to a method for determining the release rate at various points of a rumen-exposed product in ruminants, comprising the following steps: water solubility determination: weighing an appropriate amount of rumen-exposed sample and determining its water solubility; acid solubility determination: weighing an appropriate amount of rumen-exposed sample and determining its acid solubility; rumen degradation rate determination: weighing the rumen-exposed sample after water and / or acid solubility determinations, placing it on feed, adding buffer solution and rumen fluid for in vitro fermentation, drying at a constant temperature after fermentation, weighing, and determining the rumen degradation rate; abomasal degradation rate determination: weighing the rumen-exposed sample after rumen degradation rate determination, adding pepsin hydrochloride buffer solution for incubation at a constant temperature, removing and drying at a constant temperature after incubation, weighing, and determining the abomasal degradation rate; small intestinal degradation rate determination: taking the rumen-exposed sample after abomasal degradation rate determination, adding small intestinal buffer solution for shaking incubation, washing, drying at a constant temperature, weighing, and determining the small intestinal degradation rate.

The present invention relates to the technical field of medical devices, and discloses a gastrointestinal anastomotic fistula recovery and closure component, comprising: a drainage tube, an outer ring of the drainage tube is spirally wound with an adsorption sponge, the adsorption sponge comprises a dense section and a loose section, the outer edge of the dense section is in contact with the intestinal wall at the anastomotic fistula, the drainage tube is provided with adsorption holes that cooperate with the dense section, and the drainage tube is connected to a negative pressure drainage part. By using the gastrointestinal anastomotic fistula recovery and closure component described in the present invention, adsorption ports can be opened at corresponding positions of the drainage tube according to the number, length, and specific location distribution of the anastomotic fistulas, and an adsorption sponge can be spirally wound around the outer ring of the drainage tube, forming a dense section in the area near the adsorption holes and a loose section in the area between two adjacent groups of adsorption holes. After the drainage tube is placed in the intestine, multiple dense sections fill the corresponding anastomotic fistulas and the surrounding part of the intestine, and can continuously absorb secretions and digestive fluids at multiple gastrointestinal anastomotic fistulas.

The application discloses a camel special rumen internal environment monitoring device, which comprises an insertion collection component, a suction component is fixedly connected to one end of the insertion collection component, and the insertion collection component is used for inserting into a camel rumen to collect rumen fluid; a bite prevention component is fixedly arranged on the insertion collection component; and a sealing adjustment component is arranged. In the prior art, a common rumen internal environment monitoring device is contacted with an esophagus during insertion into a rumen, part of esophageal fluid or food residues enter the monitoring device, and monitoring errors are caused. By arranging the sealing adjustment component, automatic sealing protection can be realized during insertion, the sealing adjustment component can be adjusted to be opened after complete insertion into the rumen, rumen fluid can be collected and detected, and the phenomenon that other foreign matters are introduced due to contact with the esophagus during insertion is effectively avoided.

The invention relates to a bionic gastrointestinal digestive system in a lunar environment, and belongs to the technical field of bionic digestive systems. The bionic stomach digestion system, the bionic small intestine digestion system and the bionic colon fermentation system which are suspended in the water tank are arranged and are circularly connected to form the bionic gastrointestinal digestion system in the simulated moon environment; according to the astronaut gastrointestinal tract digestion simulation device, the stomach metabolism condition, the small intestine metabolism condition and the intestinal flora distribution and metabolism condition in the colon of an astronaut in a weightless environment can be simulated, the gastrointestinal tract digestion problem of the astronaut can be deeply studied, spaceflight food is developed, automation is achieved, and according to a preset program, the astronaut gastrointestinal tract digestion problem can be effectively solved. The PLC controls the addition of a sample, digestive juice and a buffer solution, and simulates the wriggling and emptying of a gastric digestive system, so that the operation is more convenient.

Provided is an isolated culture method for primary umbilical cord mesenchymal stem cells. The method comprises the following steps: S1, pretreating umbilical cord tissue; S2, adding the pretreated umbilical cord tissue to a digestive fluid containing type II collagenase, and performing digestion via shaking to obtain a digested mixed solution; S3, diluting the digested mixed solution with normal saline, performing centrifugation to collect crude precipitates, re-suspending same with normal saline, and performing centrifugation and washing to obtain precipitates; S4, transferring the precipitates into a culture solution, and performing suspension culture in a carbon dioxide incubator; and S5, transferring cells which have been subjected to the suspension culture to a sterile centrifuge tube, pipetting and mixing uniformly, performing centrifugation and washing to collect cell precipitates, and performing adherent culture on the cell precipitates to obtain primary umbilical cord mesenchymal stem cells. By means of the method, highly viable and highly proliferative primary UCMSCs can be obtained within 4–8 days from a serum-free and serum-substitute-free culture solution with clear components, thereby establishing a foundation for downstream development of new drugs and clinical research.

The present invention relates to the technical field of preparing the antibiotic tylvalosin, and provides a high-bioavailability tylvalosin dry suspension and preparation method. The preparation method comprises 5-20 parts of tylvalosin tartrate, 10-40 parts of a suspending agent, 5-20 parts of a surfactant, 10-20 parts of a carrier dispersing material, and an appropriate amount of purified water. The preparation method comprises the following steps: uniformly dispersing the formulated suspending agent with an appropriate amount of purified water, adjusting the pH to acidic with hydrochloric acid, adding a surfactant dissolved in 95% ethanol, then dissolving the drug in hydrochloric acid at pH 2.0 and adding the surfactant to the suspension, grinding the suspension in a wet nano-grinder to obtain a drug-loaded nanosuspension, adding a carrier dispersing material, and spray drying. The drug of the present invention is suspended in water for a long time at the nanoscale, releasing little drug in simulated gastric fluid but slowly in simulated intestinal fluid, thereby extending the drug's duration of action in the intestine. The surfactant increases the drug's solubility and permeability.

The invention provides a compound feed additive for ruminant rumen methane emission reduction and a verification method thereof, and relates to the technical field of feed additives and ruminant nutrition regulation and control. The compound feed additive is composed of nitroethane, fumaric acid and 3-nitrooxy propyl alcohol according to the molar ratio of 1: 1: 1, and effective action concentration is formed in rumen fermentation liquor. The method comprises the following steps: constructing a control group and a treatment group from basic ration, artificial saliva and rumen fluid by utilizing an in-vitro simulated rumen fermentation system, carrying out anaerobic culture at 39 DEG C for 24 hours, and then measuring the methane content, fermentation parameters and volatile fatty acid composition so as to evaluate the emission reduction effect. The compound feed additive disclosed by the invention can cooperatively regulate and control rumen hydrogen metabolism, keeps rumen fermentation stability while remarkably inhibiting methane generation, and provides a feasible technical approach for low-methane-emission feeding of ruminants.

The invention relates to a bionic gastrointestinal digestive system in a weightless environment, and belongs to the technical field of bionic digestive systems. The bionic stomach digestion system, the bionic small intestine digestion system and the bionic colon fermentation system which are suspended in the water tank are arranged and are circularly connected to form a bionic gastrointestinal digestion system in a simulated weightless environment; according to the astronaut gastrointestinal tract digestion simulation device, the stomach metabolism condition, the small intestine metabolism condition and the intestinal flora distribution and metabolism condition in the colon of an astronaut in a weightless environment can be simulated, the gastrointestinal tract digestion problem of the astronaut can be deeply studied, spaceflight food is developed, automation is achieved, and according to a preset program, the astronaut gastrointestinal tract digestion problem can be effectively solved. The PLC controls the addition of a sample, digestive juice and a buffer solution, and simulates the wriggling and emptying of a gastric digestive system, so that the operation is more convenient.

The invention discloses a device for capturing sheep and conveniently collecting rumen fluid, and relates to the technical field of equipment for animal experiments, and the key point of the technical scheme is that the device comprises a disc, an automatic coiling assembly is arranged in the disc, the tail end of the automatic coiling assembly is connected with one side of a T-shaped mouth gag, and the other side of the T-shaped mouth gag is connected with a motor; the other side of the T-shaped mouth gag is connected with one end of a fixing line, the other end of the fixing line is fixedly connected with the disc, a telescopic assembly is arranged at the top of the disc, and a fixing assembly is arranged at the top of the telescopic assembly. The device provided by the invention is simple to operate, does not easily cause stress of the sheep, and is convenient for taking rumen fluid.

This invention provides a magnetothermal phase change microrobot, its preparation method and application, and a targeted anchoring and sealing method, belonging to the field of biomaterials technology. The magnetothermal phase change gel provided by this invention integrates magnetically driven motion and adaptive wound filling functions, offering an effective technical means for non-invasive targeted treatment of enterocutaneous fistulas. Specifically, this invention applies a rotating magnetic field, guiding the magnetothermal phase change gel to the fistula opening and achieving initial filling under the action of a ferromagnetic body and magnetic torque. At this time, an alternating magnetic field is applied, and under the hysteresis effect, the paramagnetic body generates heat, heating the magnetothermal phase change gel to its phase transition temperature, i.e., a transition from solid to liquid, achieving adaptive and precise filling of irregular fistula openings in a fluid state. Subsequently, the alternating magnetic field is removed, and as the temperature decreases, the liquid state returns to solid, thereby tightly sealing the fistula opening and effectively preventing leakage of digestive fluids.

The utility model discloses a short-term rumen fluid in-vitro culture fermentation bag which is used for testing the influence of medicine on rumen fluid, the in-vitro culture fermentation bag comprises a nylon sealing bag with an opening, and the opening is provided with a connector sealed with a rubber plug. The rubber plug comprises a hollow silica gel column (1), an elastic silica gel membrane (2) and a protective metal tube (3) used for preventing the membrane from deforming, and the elastic silica gel membrane (2) is provided with a cross-shaped opening (4) serving as a one-way valve. According to the fermentation bag, the overall sealing performance is guaranteed, an anaerobic environment is created for rumen fluid, the rumen environment is conveniently simulated, growth of rumen contents is facilitated, meanwhile, the specially-structured one-way air guide device is adopted, air in the bag is conveniently exhausted, and the fermentation bag is used for biological experiments.

This invention discloses a method for anaerobic co-fermentation of cyanobacterial sludge to produce acid through pretreatment with bovine rumen fluid. 6–12 g of bovine rumen fluid is added to every 100 mL of the cyanobacterial sludge-water mixture, and the mixture is pretreated at 35–39°C for 12–24 h. Without removing or deactivating the bovine rumen fluid, it is mixed with inoculum sludge, and the pH of the system is adjusted to 9.5–10.5. After replacing oxygen with an inert gas, anaerobic fermentation is carried out at 35–37°C, maintaining a stirring speed of 120–150 rpm. This method can promote the dissolution of proteins and carbohydrates in cyanobacterial sludge and increase the yield of acetic acid and volatile fatty acids, making it suitable for the resource utilization of cyanobacterial sludge and the preparation of alternative carbon sources.